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Protein product and preparation method thereof

A protein and protease technology, applied in the field of protein products, to achieve the effect of low corn characteristic odor, wide application range, and broaden types

Active Publication Date: 2017-08-04
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no process for producing gliadin in an aqueous phase system completely free of organic solvents (such as ethanol, isopropanol, etc.) or high-content organic acids (such as 90% acetic acid)

Method used

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  • Protein product and preparation method thereof
  • Protein product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Water content 8.9%, albumen 64% (dry base) maize yellow powder is adjusted moisture content to 70% and enters enzymolysis tank and adjusts to pH4.8, 45 ℃, and adds the acidic protease ( MA-SD, Amano Enzyme Co., Ltd.), 60mM tris (2-carboxyethyl) phosphine reacted for 1.2 hours and adjusted to pH 7.5, 52 ° C, added 2.6% alkaline protease (2709, Pangbo Bio Engineering Co., Ltd.) and 2% neutral protease (SUKAPro NE, Sukehan Bioengineering Co., Ltd.), 1mM mercaptoethanol reacted for 0.5 hours, centrifuged, washed and collected the precipitate. The dry basis content of the total protein in the obtained product is 61.1%, the gliadin content in the protein is greater than 74%, the alpha-gliadin content is 95%, and the beta-gliadin content is 2%.

Embodiment 2

[0077] Add water to corn yellow powder with 62.4% water content and 70% protein (dry basis) to adjust to 75%, put it into an enzymolysis tank to adjust to pH 3.8, 35°C, and add acid protease (SUKAPro AC Su Cohan Bioengineering Co., Ltd.), 0.26% bromelain (food grade, Pangbo Bioengineering Co., Ltd.), 50mM sodium metabisulfite, 0.5mM tris (2-carboxyethyl) phosphine, and 4mM manganese ions were first reacted for 10 hours, Then raise the temperature to 50°C for 0.5 hours, then adjust to pH 8.3, 52°C, and add 0.33% alkaline protease (2709, Pangbo Bioengineering Co., Ltd.) to react for 1 hour, then add 10mM EDTA and centrifuge, wash and collect precipitation. The dry basis content of the total protein in the obtained product is 54.1%, and the gliadin content in the protein is 97.55%, which contains 95.85% of alpha-gliadin, 1.9% of beta-gliadin and 0.95% of gamma-gliadin.

Embodiment 3

[0079] After adjusting the water content to 80% with the corn yellow powder material containing 89.9% of water and 68% of protein (dry basis), it is put into an enzymolysis tank and adjusted to pH6.5 and 45°C, and papain (foodstuff) containing 0.01% of protein weight is added. Grade, Pangbo Bioengineering Co., Ltd.), 20mM mercaptoethanol and 0.01mM cobalt ion reacted for 1.5 hours, then adjusted to pH 10.2, 45 ℃, and added 0.01% alkaline protease (Protex 6L, Genencor Bio Engineering Co., Ltd.) after reacting for 2 hours, centrifuge, wash and collect the precipitate. The dry basis content of the total protein in the obtained product is 55.9%, and the gliadin content in the protein is greater than 90.66%, which contains 85.54% of α-gliadin, 9.91% of β-gliadin and 4.34% of γ-gliadin .

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Abstract

The invention provides a protein product and a preparation method thereof. The invention provides a method for enriching alpha-prolamin from raw materials. The raw materials comprise prolamin and non-prolamin, and optionally comprise macromolecule carbohydrate and / or grease. The method is is characterized by without using an organic solvent and comprising the following steps of (1) smashing the raw materials, and mixing pulp; (2) adopting hydrolase treatment for carrying out complete hydrolysis or partial hydrolysis on at least one part of beta-prolamin, gama-prolamin and non-prolamin in the raw materials, and utilizing a particle size difference for filtering and removing hydrolysates so as to obtain a crude product enriching the alpha-prolamin; (3) washing, dehydrating and drying the crude product, and obtaining a final protein product.

Description

technical field [0001] The invention relates to a method for enriching α-gliadin from raw materials and a method for obtaining a protein product through purification, and also relates to a protein product obtained by the method, belonging to the field of crop processing. Background technique [0002] Zein (zein) is a product derived from corn or zein-containing substances, such as corn yellow meal feed (also known as corn gluten meal, corn gluten meal) or corn distillers grains and solubles (DDGS) products extracted from. Glamin is the protein with the highest content in corn endosperm, accounting for 44wt%-79wt% of corn endosperm protein. Corn flour is mainly obtained from the wet production of corn starch. In the wet production of corn, the sulfurous acid soaked corn is first prepared, the soaking water is separated from the corn and evaporated to obtain corn steep liquor, the corn is then crushed once and twice to separate the germ, and then the endosperm is finely grou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/425C07K1/14
CPCC07K14/425
Inventor 刘泽龙
Owner COFCO NUTRITION & HEALTH RES INST
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