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Corn gluten meal functional fermented feed and application thereof

A corn gluten meal, fermented feed technology, applied in applications, animal feed, animal feed, etc., can solve the problems of low protein digestion and utilization rate, unbalanced amino acid composition, poor palatability, etc., and achieve great social value and intestinal tract. The effect of bacterial flora balance and improvement of disease resistance

Pending Publication Date: 2017-08-04
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corn gluten powder contains about 60% protein, mainly gliadin, poor solubility, unbalanced amino acid composition, poor palatability, and low digestion and utilization rate of protein currently used as feed

Method used

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  • Corn gluten meal functional fermented feed and application thereof
  • Corn gluten meal functional fermented feed and application thereof
  • Corn gluten meal functional fermented feed and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Activation of strains and preparation and cultivation of seed solution

[0038] 1) Activation of strains

[0039] Preparation of activation medium

[0040] The Bacillus natto activation medium is LB solid medium, specifically 1% tryptone, 1% sodium chloride, 0.5% yeast powder, 2% agar, and the pH value is adjusted to 7.0-7.4.

[0041] The brewer's yeast activation medium is wort solid medium. Specifically, take 1000mL of wort and use an Abbe refractometer to adjust the sugar content to 8-10Bx, agar 2%, and pH in a natural state.

[0042] The activation medium of Lactobacillus thermophiles is MRS lactic acid bacteria medium, specifically 10.0g of peptone, 10.0g of beef extract, 5.0g of yeast extract, 2.0g of diammonium hydrogen citrate, 20.0g of glucose, 801.0mL of Tween, acetic acid Dissolve 5.0 g of sodium, 2.0 g of dipotassium hydrogen phosphate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, and 18.0 g of agar in 1000 mL of distilled water, and adjust th...

Embodiment 2

[0049] Combination of three strains of Bacillus subtilis, Bacillus coagulans and Bacillus natto for solid fermentation

[0050] Medium preparation requirements: corn gluten powder: bran = 7:3; material (ie, the total weight of corn gluten powder and bran): water is mixed in a ratio of 1g:1.2ml to obtain a fermentation medium.

[0051] The inoculum amount of bacteria is 10%, and the ratio of the number of live cells of Bacillus subtilis, Bacillus coagulans and Bacillus natto is 1:1:1, and they are inoculated into the fermentation medium for fermentation and cultivation. The temperature of fermentation cultivation is 30° C., the time of fermentation and cultivation is 120 h, and the fermented feed is obtained.

Embodiment 3

[0053] Combination of three strains of Bacillus subtilis, thermophilic lactic acid bacteria and Bacillus natto for solid fermentation

[0054] Medium preparation requirements: corn gluten powder: bran ratio is 6:4; materials (that is, the total weight of corn gluten powder and bran) and water are mixed according to the ratio of 1g:1.4mL to obtain the fermentation medium.

[0055] The inoculum amount of bacteria is 8%, and the ratio of the number of live bacteria of Bacillus subtilis, thermophilic lactic acid bacteria and Bacillus natto is 1:0.5:1.5 to inoculate into the fermentation medium for fermentation and cultivation, and the temperature of fermentation and cultivation is 32°C, the time of fermentation and cultivation is 108h, and the fermented feed is obtained.

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Abstract

The invention provides corn gluten meal functional fermented feed and a preparation method and application thereof and belongs to the technical field of feed. The corn gluten meal functional fermented feed is a fermentation product of a culture medium containing corn gluten meal and bran under fermentation action of three bacteria. The three bacteria include bacillus subtilis and two of bacillus coagulans, thermophilic lactobacillus, bacillus natto and bear yeast. Soluble protein content in the fermented feed is increased from 3.74% to 270% at most; the novel microbial fermentation feed prepared by adding highly-symbiotic bacteria can bring about more economic benefit and has great social value. By applying the corn gluten meal functional fermented feed in animal raising, growing performance of raised animals is improved effectively while own immunity of the animals can be improved.

Description

technical field [0001] The invention belongs to the technical field of feed, in particular to corn gluten powder functional fermented feed and application thereof. Background technique [0002] With the development of my country's animal husbandry, the contradiction of protein feed shortage has become increasingly prominent. The main way to solve this contradiction is to develop new feed resources and rationally utilize existing feed resources. The production of high-protein feed by microbial fermentation is one of the effective ways to solve the shortage of feed protein resources. [0003] Corn is one of the three major food crops in the world today. In 2015, my country's corn planting area was 38,116.6 thousand hectares, with a unit yield of 5891.9Kg / ha, slightly higher than the world average. The main component of corn is starch, up to 75%, so corn plays an important role in starch production. At present, the main method of extracting corn starch in China is wet extrac...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K20/147A23K50/30
CPCY02P60/87
Inventor 刘晓兰江成英王燕郑喜群任健
Owner QIQIHAR UNIVERSITY
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