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Titled extracts of cynara scolymus and uses thereof

A technology of extract and artichoke, applied in the field of artichoke titration extract and its application

Inactive Publication Date: 2017-08-01
ABOCA S P A SOC AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite initial positivity about the efficacy of these treatments, physicians must immediately confront the problem of relapse, as those patients with cancer almost always develop resistance to the drugs, often due to the activation of other pathways

Method used

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  • Titled extracts of cynara scolymus and uses thereof
  • Titled extracts of cynara scolymus and uses thereof
  • Titled extracts of cynara scolymus and uses thereof

Examples

Experimental program
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preparation example Construction

[0143] The preparation of such extracts is generally known to those skilled in the art and need not be described in particular detail in this disclosure. For the purposes of the present invention, any of the above-identified extracts prepared according to conventional techniques may be used.

[0144] In particular, for the purposes of the present invention, the extract may also be replaced by a fraction or a mixture of fractions of the artichoke extract described above or a mixture of an artichoke extract and more than one extract fraction, provided that the above disclosed titration Standard is fine.

[0145] Considering the variability in plant extracts that can arise from climatic conditions, environmental conditions, from differences in cultivation sites and / or cultivation techniques, or even from different varieties of cultivated plants, it is therefore important for clinical use that Standardizing the product and determining standardized parameters enables the provision...

Embodiment

[0277] 1. Phosphorylation of STAT3 was analyzed by Western blot. The results are reported in Figures 1-3.

[0278] 1.1. Cell Lysis and Western Blotting.

[0279]Lysis buffer NP40 (50mM Tris-HCl pH 7.4, 150mM NaCl, 1% NP-40, 1mM EGTA, 1mM EDTA) supplemented with protease and phosphatase inhibitors (5mM PMSF, 3mM NaF, 1mM DTT, 1mM NaVO4) ) for 30 min on ice. Equal amounts of total protein extracts (30 μg) were resolved by denaturing electrophoresis on 8% polyacrylamide gels (SDS-PAGE) and transferred to nitrocellulose membranes over 2 hours. Membranes were blocked with 5% milk solution dissolved in TBS-Tween_200.05% for 1 hour and incubated with specific primary antibodies. The following primary antibodies were used: anti-β-actin (A-2228, SIGMA), anti-pSTAT3 (Tyr-705) (sc8059, Santa Cruz) and anti-STAT3 (sc7179, Santa Cruz). Secondary antibodies were peroxidase-conjugated (Santa Cruz) and used for chemiluminescence using ECL reagents (Amersham, GE Healthcare, Piscataway, NJ,...

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Abstract

The present invention relates to a titrated extract of Cynara scolymus, to titrated fractions of extract of Cynara scolymus or titrated mixtures of the extract with one or more of the titrated fractions or mixtures of the fractions, and to compositions and kits comprising them, for the prevention and / or the treatment of a pathological condition characterised by a constitutive activation of the STAT3 transcription factor.

Description

technical field [0001] The present invention relates to a titrated extract of cardoon, a titrated fraction of a cardoon extract, or a titrated mixture of said extract and one or more of said titrated fractions, or a titrated fraction of said extract, in combination with more than one chemotherapeutic or anti-inflammatory drug. Mixtures, as well as compositions and kits comprising them, for the prevention and / or treatment of pathological states characterized by constitutive activation of the STAT3 transcription factor. Background technique [0002] In recent decades, much evidence in the literature suggests a fundamental role for transcription factors belonging to the STAT family in a variety of conditions, such as tumor-promoting inflammatory conditions and in tumors themselves. STAT proteins are cytoplasmic transcription factors whose phosphorylation / activation (at specific residues at serine and / or tyrosine due to the action of the JAK or Janus kinase family of proteins) d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K36/28A61K31/519A61K31/704A61P35/00
CPCA61K31/519A61K31/704A61K36/28A61K45/06A61K31/192A61K31/216A61K31/343A61K2300/00A61K2236/00A61K31/365A61P11/00A61P29/00A61P35/00A61P35/02A61P43/00
Inventor 瓦伦蒂诺·梅尔卡迪
Owner ABOCA S P A SOC AGRI
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