Chinese yam soluble dietary fiber and preparation method thereof
A dietary fiber and soluble technology, which is applied to the field of yam soluble dietary fiber and its preparation, can solve the problems of equipment corrosion, long extraction time, high equipment requirements, etc., and achieves the effect of fewer operation steps, suitable for industrial amplification, and good solubility.
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Embodiment 1
[0036] 1) Extraction: crush 1kg of yam, add water 50 times that of yam, and extract at 60°C for 15 minutes.
[0037] 2) Enzymolysis: Add compound enzymes to the extract obtained in step 1). The compound enzymes include papain, neutral protease, glucoamylase, cellulase, and hemicellulase, and the amounts of enzymes are the quality of Chinese yam 1%, 1.5%, 2%, 1.6%, 0.8% of . Enzymolysis was carried out at 40°C for 2 hours, the pH of the enzymolysis was 8, solid-liquid separation was carried out, and the supernatant was collected.
[0038] 3) Concentration: The clear liquid obtained in step 2) is subjected to ultrafiltration and concentration, and the molecular weight cut-off of the ultrafiltration membrane is 3kD. The concentrate obtained in step 3) is dried to obtain yam soluble dietary fiber.
Embodiment 2
[0040] 1) Extraction: crush 1kg of Chinese yam, add water 5 times that of Chinese yam, and extract at 30°C for 120min.
[0041] 2) Enzymolysis: Add compound enzymes to the extract obtained in step 1). The compound enzymes include papain, neutral protease, glucoamylase, cellulase, and hemicellulase, and the amounts of enzymes are the quality of Chinese yam 0.1%, 0.2%, 0.2%, 0.1%, 0.1%. Enzymolysis was carried out at 15°C for 4 hours, the pH of the enzymolysis was 6, solid-liquid separation was carried out, and the supernatant was collected.
[0042] 3) Concentration: The clear liquid obtained in step 2) is subjected to ultrafiltration and concentration, and the molecular weight cut-off of the ultrafiltration membrane is 10kD. The obtained concentrate is dried to obtain yam soluble dietary fiber.
Embodiment 3
[0044] 1) Extraction: crush 1kg of Chinese yam, add water 30 times that of Chinese yam, and extract at 50°C for 30 minutes.
[0045] 2) Enzymolysis: Add compound enzymes to the extract obtained in step 1). The compound enzymes include papain, neutral protease, glucoamylase, cellulase, and hemicellulase, and the amounts of enzymes are the quality of Chinese yam 0.5%, 0.2%, 1.0%, 0.3%, 0.3%. Enzymolysis was carried out at 20°C for 3 hours, the pH of the enzymolysis was 7.5, solid-liquid separation was carried out, and the supernatant was collected.
[0046] 3) Concentration: The clear liquid obtained in step 2) is subjected to ultrafiltration and concentration, and the molecular weight cut-off of the ultrafiltration membrane is 5kD. The obtained concentrate is dried to obtain yam soluble dietary fiber.
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