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Method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms

A technology of dimethylformamide and microbial degradation, applied in the field of N,N-dimethylformamide, which can solve the problems of high cost and difficult treatment of DMF wastewater, and achieve simple daily maintenance and operation and low equipment requirements Low, easy-to-operate effect

Active Publication Date: 2017-07-28
GUANGDONG FOOD IND INST +1
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Problems solved by technology

[0005] In order to solve the problem of high cost and difficulty in the treatment of low-concentration DMF wastewater in the industrial production of sucralose in the prior art, the present invention provides a method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms Method, the method can realize high efficiency and low-cost treatment of N,N-dimethylformamide in sucralose wastewater

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  • Method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms
  • Method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms
  • Method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms

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Embodiment 1

[0041] 1. Enrichment culture of microbial flora

[0042] 1) Weigh 2 g of tryptone, 1 g of yeast extract, and 2 g of sodium chloride, add water to 200 mL, adjust the pH to 7.0, and then sterilize under high temperature and high pressure (sterilization conditions: 121°C, 20 minutes).

[0043] 2) Inoculate microbial colonies into the above-mentioned culture solution and culture for 24 hours. The culture conditions are: temperature 30°C, DO=3mg / L, to obtain enriched culture solution.

[0044] The microbial flora can grow rapidly in the above-mentioned medium, so that the concentration of the microbial flora reaches a high level, and the enrichment culture of the microbial flora is conducive to the rapid start of the next bioreactor.

[0045] The microbial flora was Pseudomonas aeruginosa (No. GIM1.843) purchased from Guangdong Microbial Culture Collection Center.

[0046] 2. Domestication of microbial flora

[0047] The domestication of microbial flora is carried out in a biore...

Embodiment 2

[0061] 1. Screening of microbial flora from sludge of sucralose production sewage plant

[0062] (1) Weigh 1 g of disodium hydrogen phosphate, 0.5 g of potassium dihydrogen phosphate, and 0.02 g of sodium chloride, and add water to 200 mL. Inoculate 5g of sucralose production sewage plant sludge, the culture condition is 25-35°C, the dissolved oxygen is 1-3mg / L, the pH is 7.0-8.0, and the culture time is 5 days.

[0063] Take 10mL (5-10% ratio, volume percentage) from the above culture solution and transfer it to fresh screening culture solution. The fresh culture solution includes disodium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 2.5g / L, chlorine Sodium chloride 0.1g / L, cultivated for 5 days.

[0064] (2) Repeat step (1) for 6 times to obtain a microbial flora that can be grown with DMF as the only carbon source and nitrogen source. This microbial flora includes Pseudomonas and parasitoids through routine physiological and biochemical identification and gene s...

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Abstract

The invention discloses a method for degrading N,N-dimethylformamide in sucralose wastewater by using microorganisms. The method comprises: (1) enrichment culture of microbial flora: selecting a microbial flora, inoculating into a culture liquid, and carrying out enrichment culture to obtain an enrichment culture liquid; (2) acclimation of the microbial flora: selecting the enrichment culture liquid, inoculating into an initial acclimation culture liquid containing N,N-dimethylformamide, and carrying out acclimation on the microbial flora by continuously adjusting the N,N-dimethylformamide content in a biological reaction device so as to obtain an acclimation completing culture liquid; and (3) degradation of N,N-dimethylformamide in sucralose wastewater with the microorganisms: continuously introducing sucralose production wastewater containing N,N-dimethylformamide into the acclimation completing culture liquid, and degrading the N,N-dimethylformamide in the sucralose wastewater by using the microorganisms. With the method of the present inventin, the high efficiency and low cost treatment of the N,N-dimethylformamide in the sucralose wastewater can be achieved.

Description

technical field [0001] The invention belongs to the technical field of sucralose wastewater treatment, and in particular relates to a method for using microorganisms to degrade N,N-dimethylformamide in sucralose wastewater. Background technique [0002] N, N-dimethylformamide (N, N-dimethylformamide, DMF) is a colorless organic solvent widely used in the production of various chemical industries, known as "universal solvent". DMF can enter the human body through breathing and skin contact, causing various degrees of damage to the human body. According to the survey, long-term exposure to or inhalation of DMF can easily lead to symptoms such as abnormal liver function, loss of appetite and abdominal distension. Since DMF only exists as a solvent in the process of chemical production, it is basically discharged together with the production wastewater without being consumed. Therefore, how to effectively treat DMF wastewater is a difficult problem faced by many chemical produc...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12N1/02C02F101/38
CPCC02F3/34C02F2101/38C12N1/02C12N1/20
Inventor 韩诗蕾李春荣马芬丽胡宇凌廖金华
Owner GUANGDONG FOOD IND INST
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