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Industrialized preparation method of pawpaw enzyme preparation, and applications thereof

A technology of papaya enzyme and papaya, applied in the field of industrial preparation of papaya enzyme preparations, to achieve the effects of reduced water and electricity consumption, stable product quality, and prominent sour taste

Inactive Publication Date: 2017-07-04
DONGGUAN HUAQI BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CPL has been reported to be used in the catalytic synthesis of artificial breast milk substitutes, structural esters, aromatic esters, biodiesel and chiral resolution of pharmaceutical intermediates, etc., but there is no report on the application of CPL in the industrial production of milk flavor bases

Method used

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  • Industrialized preparation method of pawpaw enzyme preparation, and applications thereof
  • Industrialized preparation method of pawpaw enzyme preparation, and applications thereof
  • Industrialized preparation method of pawpaw enzyme preparation, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Please refer to figure 1 , the present embodiment provides a kind of industrialized preparation method of papaya enzyme preparation, comprises the following steps:

[0037] (1) Set VW-0.42 / 7 oil-free air compressor 1 (discharge pressure 0.7Mpa, volume flow 0.42m3 / min), A27W-10T spring safety valve (TS) 2, with 3251.072.090 pressure sensor 31 and LZJ glass rotameter 32 50L storage tank 3, pressure gauge 4, BMS0.5 / 290-UB reinforced polypropylene plate and frame filter press 5 (filter area 0.5m 2 , the total volume of the filter chamber is 7.5L), 80 mesh filter cloth, return pipe 6, the first spray dryer 7, and the second spray dryer 8; the spring safety valve 2 is connected to the oil-free air compressor 1 and the plate frame filter press 5, the pressure gauge 4 is respectively connected to the storage tank 3 and the plate and frame filter press 5, the storage tank 3 is also connected to the plate and frame filter 5 through the return pipe 6, and the 80-mesh filter cloth...

Embodiment 2

[0048] This example is basically the same as Example 1, the difference being that the inlet temperature in step (5) of the industrialized preparation method of papaya enzyme preparation is 125° C., and other steps are the same as in Example 1, and will not be repeated here.

Embodiment 3

[0050] This example is basically the same as Example 1, the difference being that the inlet temperature in step (5) of the industrialized preparation method of papaya enzyme preparation is 115° C., and other steps are the same as Example 1, and will not be repeated here.

[0051] The papaya lipase that embodiment 1-3 is made contrasts, compares the influence of different material inlet temperatures on the preparation of papaya lipase, calculates yield, specific enzyme activity, lipase recovery rate and water content, wherein, yield ( %)=spray-dried papaya lipase weight / weight of papaya milk×100%, enzyme activity recovery (%)=(spray-dried papaya lipase weight*specific enzyme activity) / (weight*specific enzyme activity of papaya milk)× 100%, list the results as follows:

[0052]

[0053]

[0054] It can be seen from the above table that the higher the material inlet temperature, the higher the yield of CPL and the higher the total recovery of lipase. The higher the inlet t...

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PUM

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Abstract

The invention discloses an industrialized preparation method of a pawpaw enzyme preparation. According to the industrialized preparation method, plate filtration and spray drying are adopted to separate and prepare pawpaw lipase and papain; the preparation method is scientific; the process is convenient to control; material utilization rate is high. Compared with a pawpaw lipase preparation method wherein water soluble proteases in pawpaw milk are removed via repeat washing with buffer solution, the industrialized preparation method comprises following advantages: yield is high; enzyme activity recovery rate is high; pawpaw lipase and papain can be extracted at the same time; water and electricity consumption is reduced greatly; the industrialized preparation method is especially suitable for industrialized production. The invention also discloses a method used for preparing an enzymolysis milk flavor essence using the pawpaw lipase. According to the method, single cream, PBS buffer solution, and glycerin monostearate are mixed at a certain ratio, an obtained mixture is subjected full stirring emulsification at 60 to 80 DEG C, pawpaw lipase is added for enzymatic hydrolysis at 30 to 60 DEG C, and an obtained product is heated to 85 to 90 DEG C for 30min for enzyme deactivation so as to obtain the pawpaw lipase enzymolysis milk flavor essence. The pawpaw lipase enzymolysis milk flavor essence is unique in flavor, and can be used for preparing milk flavor products with unique flavor.

Description

technical field [0001] The invention relates to an industrialized preparation method of lipase and its application, in particular to an industrialized preparation method of papaya enzyme preparation and its application. Background technique [0002] Papaya milk contains rich enzymes such as papain, papain B, chymopapain A, and chymopapain B, among which papain has been widely used in food, medicine, daily chemical and other industries. In 1991, Giordani et al. first reported that papaya milk has the function of hydrolyzing triglycerides, and found that the main component with hydrolysis function is the water-insoluble component in papaya milk, that is, papaya lipase (Carica papaya lipase, CPL). Because it is embedded in the form of a matrix with the water-insoluble rubber-like substance in papaya, it is called "natural immobilized enzyme". [0003] Currently, CPL is a by-product of papain production, and the presence of CPL in solid form reduces the purity of papain. CPL i...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/20A23L27/20
Inventor 吴新良林影张锟
Owner DONGGUAN HUAQI BIOLOGICAL SCI & TECH
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