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A method for culturing Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room

A natural light source, Dendrobium Huoshanense technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of lack of "light quality", achieve the effect of facilitating absorption, uniform reflection, and improving product quality

Active Publication Date: 2019-01-25
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The tissue culture seedlings of Dendrobium Huoshanense are more cultivated in the tissue culture room. However, the traditional tissue culture room is mostly adjusted for the "light intensity" and "photoperiod" required for plant growth, and lacks the important factor of "light quality".

Method used

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  • A method for culturing Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room
  • A method for culturing Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room
  • A method for culturing Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room

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Embodiment 1

[0043] Such as figure 1 As shown, a method for cultivating Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room in this embodiment comprises the following steps:

[0044] (1) Germination of seeds

[0045] ① prepare seed germination medium (MS medium, formula is: every liter of medium contains white granulated sugar 50g, agar 4.5g, pH value 6.1), after completion, put it into a culture bottle and carry out autoclave sterilization for later use;

[0046] ②Take the fruit of Dendrobium Huoshanense, sterilize it with 73% ethanol solution for 1 minute, then soak it with 0.1% mercuric acid solution for 15 minutes, then wash and soak it with sterile water for 3 times, remove it and put it on sterile filter paper, cut the fruit, Shaking off the powdered seeds onto the seed germination medium for inoculation;

[0047] ③ Place the inoculated culture medium in a natural light source tissue culture room, adjust the ratio of red and blue light r...

Embodiment 2

[0060] Such as figure 1 As shown, a method for cultivating Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room in this embodiment comprises the following steps:

[0061] (1) Germination of seeds

[0062] 1. prepare seed germination medium (MS medium, formula is: every liter of medium contains white granulated sugar 50g, agar 4.5g, pH value 6), put into culture bottle after completion and carry out autoclave sterilization stand-by;

[0063] ②Take the fruit of Dendrobium Huoshanense, sterilize it with 70% ethanol solution for 0.5 minutes, then soak it with 0.1% mercuric acid solution for 10 minutes, then wash and soak it twice with sterile water, remove it and put it on sterile filter paper, cut the fruit, Shaking off the powdered seeds onto the seed germination medium for inoculation;

[0064] ③ Place the inoculated culture medium in a natural light source tissue culture room, adjust the ratio of red and blue light radiation in the...

Embodiment 3

[0073] Such as figure 1 As shown, a method for cultivating Dendrobium huoshanense tissue culture seedlings using a natural light source tissue culture room in this embodiment comprises the following steps:

[0074] (1) Germination of seeds

[0075] 1. Prepare seed germination medium (MS medium, formula is: every liter of medium contains 50 g of white granulated sugar, 4.5 g of agar, pH 6.2), and after completion, put it into a culture bottle and carry out autoclave sterilization for later use;

[0076] ②Take the fruit of Dendrobium Huoshanense, sterilize it with 75% ethanol solution for 1.5 minutes, then soak it with 0.1% mercuric chloride solution for 20 minutes, then wash and soak it with sterile water for 4 times, remove it and put it on sterile filter paper, cut the fruit, Shaking off the powdered seeds onto the seed germination medium for inoculation;

[0077] ③ Place the inoculated culture medium in a natural light source tissue culture room, adjust the ratio of red an...

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Abstract

The invention discloses a method for cultivating tissue cultured seedlings of Dendrobium huoshanense in a tissue culture room using a natural light source, comprising the following steps: (1) germination of seeds; (2) cultivation of round bulbs; (3) cultivation of seedlings; (4) high-quality dendrobium huoshanense Obtaining test tube seedlings. The advantage of the present invention is that: through the self-made intelligent greenhouse based on electrochromic glass, the radiation ratio and radiation intensity of red and blue light waves inside the greenhouse can be adjusted as a whole or in sections, so as to meet the growth and development requirements of Dendrobium Huoshanense at different stages. To meet the demand for quality, promote plant growth and development, and improve product quality, thereby providing a method for cultivating Dendrobium huoshanense tissue culture seedlings with simple operation, convenient management, and high product quality.

Description

technical field [0001] The invention relates to a method for cultivating tissue-cultured seedlings of Dendrobium huoshanense, in particular to a method for cultivating tissue-cultured seedlings of Dendrobium huoshanense in a tissue culture room using a natural light source. Background technique [0002] Dendrobium is a perennial herb of the family Orchidaceae. Huoshan Dendrobium, commonly known as "Mihu", is mainly distributed in Huoshan in the southwest of Anhui. Because of its alkaloids, crude polysaccharides, amino acids, trace elements and other components, it has the functions of strengthening kidney and essence, thickening stomach, inhibiting tumor cells, lowering blood sugar, It has been regarded as the first of the nine "immortal herbs" for its effects of enhancing immunity and relieving physical fatigue. [0003] The tissue culture seedlings of Dendrobium Huoshanense are more cultivated in the tissue culture room. However, the traditional tissue culture room is mos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓辉陈乃富韦传宝孙立王寅嵩
Owner WEST ANHUI UNIV
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