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Urea detection reagent with excellent detection line and analysis sensitivity and detection method

A technology for detection reagents and detection methods, applied in biological testing, material inspection products, etc., can solve the problems of poor accuracy, difficulty in comprehensive application, high cost of reagents, etc., to achieve improved stability, good performance, superior detection limit and analysis The effect of sensitivity

Inactive Publication Date: 2017-06-23
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have poor accuracy, low sensitivity, high requirements for instruments and equipment, and high cost of reagents, making it difficult to fully popularize and apply them.

Method used

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  • Urea detection reagent with excellent detection line and analysis sensitivity and detection method
  • Urea detection reagent with excellent detection line and analysis sensitivity and detection method
  • Urea detection reagent with excellent detection line and analysis sensitivity and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] UREA detection reagents, including reagent R1 and reagent R2:

[0036] 1) The components of reagent R1 are:

[0037] Buffer (pH 9.1) ··············································· 100mmol / L

[0038] NADPH ························································· 0.3mmol / L

[0039] 2) The components of reagent R2 are:

[0040] Buffer (pH 7.6) ··············································· 100mmol / L

[0041] Urease ·························································· 40KU / L

[0042] glutamate dehydrogenase ·················································· 2KU / L

[0043] alpha-ketoglutarate ···················································· 30mmol / L

[0044] preservative ························································ 0.8g / L;

[0045] 3) The method of using the reagents in this example:

[0046] The UREA detection reagent described in this example is used with a fully automatic biochemical analyzer with dual reagent functions, such as the ...

Embodiment 2

[0051] Interference test: take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then the reagents obtained in Example 1 were used to compare and measure the content of UREA in serum at the same time as the common and recognized UREA reagents in the market. The results of the control group and the results of each group after adding different interfering substances are shown in Table 2. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0052] It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid ≤ 1704 μmol / L, bilirubin ≤ 684 μmol / L, hemoglobin ≤ 5 g / L, and triglyceride ≤ 22.6 mmol / L. However, the reagents of the control group were signifi...

Embodiment 3

[0056] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the UREA kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 3 . And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9974, indicating that there is a great correlation between the two.

[0057] Table 3 Comparative test results of the reagents in Example 1 and the common and recognized UREA assay kits in the market

[0058]

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Abstract

The invention relates to the technical field of UREA detection, in particular to a UREA detection reagent. Reagent R1 contains tris buffer, NADPH, and a preservative; reagent R2 contains tris buffer, urease, gluten Acid dehydrogenase, alpha-ketoglutarate, XOD, POD, preservatives. The improved enzymatic urea reagent has superior detection limit and analytical sensitivity, and its own performance is good, which greatly enhances the stability of the reagent and meets the relevant clinical application requirements.

Description

technical field [0001] The invention relates to the technical field of urea detection, in particular to a urea detection reagent with superior detection line and analytical sensitivity, and also to a detection method using the detection reagent. Background technique [0002] Urea (UREA) is the end product of human protein metabolism. Amino acids in the body are decomposed into α-keto acids and NH by deamination 3 , NH 3 Enter the urea cycle and CO in hepatocytes 2 This produces urea. The amount of urea produced depends on dietary protein intake, tissue protein catabolism, and liver function status. The generated urea is mainly excreted by the kidneys through the blood circulation. Most of the urea discharged into the digestive tract through saliva, gastric juice, bile and intestinal juice is decomposed into NH 3 After absorption, urea is synthesized by the liver and excreted by the kidneys. The urea in the plasma can be completely filtered from the glomerulus, and und...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/62
CPCG01N33/62
Inventor 甘宜梧史秀明谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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