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Method for testing content of calcium ions in calcium-magnesium water body

A test method, calcium ion technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of inability to popularize and use, high equipment cost, low sensitivity, etc., and achieve easy implementation and wide promotion and use, simple equipment, low cost, and simple operation easy effect

Inactive Publication Date: 2017-06-23
厦门澳汤维尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of poor test accuracy, low sensitivity, poor precision, high equipment cost, difficult implementation and unpromotable use of the above-mentioned prior art, the present invention adopts the following technical solutions:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The curve is prepared in the early stage, the curve is established once, and there is no need to establish it again under the same wavelength in the future;

[0022] 1. Pipette 0, 1, 2, 3, 4, 5mL calcium standard solution (20ug / mL) into a 10mL colorimetric tube, and accurately add 1mL solution A, which contains 2x10-2 M 1:1 Zn-EGTA, pH6 2M boric acid buffer solution. Add 1 mL of 2x10-3 M PAN solution. Among them, the PAN solution is ready-to-use and ready-to-use;

[0023] 2. Dilute to 10mL with water and shake well. Leave it at room temperature for 10 minutes to develop color, put it into a cuvette, and measure the absorbance at a wavelength of 525 nm with the reagent blank as a reference;

[0024] 3. Establish a standard curve according to the standard calcium solution;

[0025] Measurement:

[0026] 1. Direct measurement of a certain brand of mineral water;

[0027] Add 1mL solution A to 2.8mL mineral water, 1mL 2x10-3 M PAN solution;

[0028] 2. Place the fina...

Embodiment 2

[0030] The curve is prepared in the early stage, the curve is established once, and there is no need to establish it again under the same wavelength in the future;

[0031] 1. Pipette 0, 1, 2, 3, 4, 5mL calcium standard solution (20ug / mL) into a 10mL colorimetric tube, and accurately add 1mL solution A, which contains 2x10-2 M 1:1 Zn-EGTA, pH6 2M boric acid buffer solution;

[0032] 2. Add 250mg of PAN-NaCl mixture, in which PAN:NaCl is 1:249, that is, 1g of PAN and 249g of NaCl are mixed evenly, after PAN-NaCl is mixed, PAN forms sodium salt, its solubility is greatly increased, and it can be dissolved without alcohol in water;

[0033] 3. Dilute to 10mL with water and shake well. Place at room temperature for 10 minutes to develop color, put it into a cuvette, and measure the absorbance at a wavelength of 570nm with the reagent blank as a reference;

[0034] 4. Establish a standard curve according to the standard calcium solution;

[0035] Measurement:

[0036] 1. Take ...

Embodiment 3

[0041] The curve is prepared in the early stage, the curve is established once, and there is no need to establish it again under the same wavelength in the future;

[0042] 1. Pipette 0, 1, 2, 3, 4, 5mL calcium standard solution (20ug / mL) into a 10mL colorimetric tube, and accurately add 1mL solution A, which contains 2x10-2 M 1:1 Zn-EGTA, pH6 2M boric acid buffer solution;

[0043] 2. Add 100mg of PAN-NaCl mixture, in which PAN:NaCl is 1:99, that is, 1g of PAN and 99g of NaCl are mixed evenly, after PAN-NaCl is mixed, PAN forms sodium salt, its solubility is greatly increased, and it can be dissolved without alcohol in water;

[0044] 3. Dilute to 10mL with water, shake well; place at room temperature for 10 minutes to develop color, put it into a cuvette, and measure the absorbance at a wavelength of 570nm with the reagent blank as a reference;

[0045] 4. Establish a standard curve according to the standard calcium solution;

[0046] Measurement:

[0047] 1. Take 0.2mL ...

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Abstract

The invention discloses a method for testing content of calcium ions in calcium-magnesium water body. The method comprises the following steps: selecting 0.1-3*10<-2>M Zn solution, 0.1-3*10<-2>M EGTA solution, Zn-EGTA solution which is obtained by mixing and diluting the Zn solution and the EGTA solution to the needed concentration according to equal volume, 0.1-3*10<-3>M PAN ethanol solution, buffering solution, namely 1-2M boric acid solution with the pH of 5-10 which is controlled by by adopting NaOH and 10-100mug / mL Ca standard solution, and determining the ionic calcium by adopting a Zn-EGTA competition method, so that the testing precision is high, the sensitivity is good, the equipment is simple and low in cost as a common spectrometer is needed; and the operation is simple and convenient, and the method is easy to realize and wide to popularize and use.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a method for testing calcium ions in water in an aquarium. Background technique [0002] Aquariums will produce certain calcium ions during the aquarium breeding process. Calcium ions will have a greater impact on the production of aquariums. Calcium ions need to be tested and removed at any time. Testing the content of calcium ions is a very important step. For now , Calcium ion testing basically adopts EDTA titration, atomic absorption spectrophotometry, isotope dilution mass spectrometry, etc. These methods can meet certain testing requirements, but there are also relatively large defects, such as poor test accuracy and poor sensitivity of EDTA titration , low precision, while atomic absorption spectrophotometry and isotope dilution mass spectrometry require very sophisticated equipment, and the cost of equipment is high, which is unrealizable for most enterprises and users, and is ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 秦骥
Owner 厦门澳汤维尔生物科技有限公司
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