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Hemoglobin detection method and hemoglobin detection kit

A detection kit and hemoglobin technology, applied in the field of biotechnology analysis and detection, can solve problems such as increasing errors, and achieve the effects of accurate results, fast response, and optimized sample dilution

Inactive Publication Date: 2017-06-20
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for the immunological detection of hemoglobin, it generally requires multi-step dilution to make it within the linear range, but the error caused by this is bound to increase

Method used

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  • Hemoglobin detection method and hemoglobin detection kit
  • Hemoglobin detection method and hemoglobin detection kit
  • Hemoglobin detection method and hemoglobin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of hemoglobin detection kit that realizes above-mentioned detection method, comprises by hemoglobin detection test paper (such as Figure 4 shown), external card case (such as Figure 5 shown), the sample lysate and the IC card with the corresponding standard curve and determination method inside, among which,

[0030] The hemoglobin detection test paper has a width of 3-5mm and a length of 7-8cm. The detection test paper is composed of a colloidal gold pad 1 containing a colloidal gold-labeled anti-human hemoglobin antibody, coated with a T line 2 and a C line 3 (ie Coated with paired hemoglobin antibody and anti-mouse IgG) nitrocellulose membrane 4 (NC membrane, a porous structure membrane with a pore size of 8-15um), a sample pad 5, a suction pad 6 and a plastic bottom plate (not shown) , the T line is paired hemoglobin antibody, the C line is anti-mouse IgG, the anti-human hemoglobin antibody is labeled with colloidal gold particles as the first antibody, an...

Embodiment 2

[0040] 1 material

[0041] 1.1 The hemoglobin detection kit described in Example 1 of the present invention

[0042] 1.2 Hb enterprise internal control product: human hemoglobin dry powder, purchased from Sigma, product number: H7379, weigh the dry powder and dilute it to a high concentration with pure water, and then dilute it to 0.05g / L, 0.1g / L, 0.1g / L, 0.5g / L, 1g / L, 5g / L, 20g / L. 50g / L, 100g / L, 150g / L, 200g / L, and 250g / L of the internal control products of the enterprise are human-defined whole blood.

[0043] 1.3 Clinical samples: obtained by the company in relevant hospitals, a total of 120 samples, of which 60 were whole blood samples from patients diagnosed with anemia, and the Hb detection value was from 60-90g / L. 60 normal human serum samples, Hb detection value from 110-160g / L.

[0044] 1.3 Experimental equipment: NS3001B immunochromatographic result interpretation recorder of Tianjin Zhongxin Keju Biopharmaceutical Co., Ltd.

[0045] 2 methods

[0046] Concentra...

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Abstract

The invention provides a hemoglobin detection method and a hemoglobin detection kit. When the concentration of hemoglobin in a sample reaches certain amount, a HOOK effect is formed, and is enhanced along with the continuous increasing of the concentration, and a reverse inhibition effect is formed, and is regularly enhanced within certain range; by studying a hook-shaped area of the HOOK effect of a hemoglobin double-antibody sandwich, the sample thinning is optimized, and the difficult point of failure to directly detect due to HOOK effect in the immunochromatography technique is innovatively broken through, so as to accurately and quickly detect the content of the hemoglobin, fill the blank in the field, and facilitate the site detection and family detection. The detection method has the advantage that by adopting the method of further thinning and cracking a blood sample, the content of the hemoglobin within the obtainable range of a human body is well controlled to present a good dose curve rule in the HOOK effect area.

Description

technical field [0001] The invention relates to the field of biotechnology analysis and detection, in particular to a hemoglobin detection method and a hemoglobin detection kit. Background technique [0002] At this stage, the detection methods of hemoglobin include: copper sulfate titration for hemoglobin determination, or the use of imported large-scale biochemical analyzers for determination. The former has disadvantages such as large errors in self-preparation and being easily affected by ambient temperature, while the latter has expensive instruments and high testing costs. The commonly used test methods in foreign countries are kits and dry biochemical analysis methods. The kit method requires a large biochemical analyzer. The analyzers all use the wavelength colorimetric method, which requires a large amount of blood collection, long test time, and high cost. [0003] The advantages of immunochromatographic colloidal gold technology are minimally invasive sampling, ...

Claims

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Application Information

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IPC IPC(8): G01N33/559G01N33/72
CPCG01N33/559G01N33/721
Inventor 李昀地周洪锐杨延瑞张梓琪陈逸桐宋兆瑞李洲
Owner 天津中新科炬生物制药股份有限公司
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