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Novel function of N-acetylchitobiose and application of N-acetylchitobiose in health care food

A technology of health food and chitobiose, which is applied in the field of health food, can solve the problems of limited research on the anti-diabetic function of chito-oligosaccharides and no research reports on anti-type II diabetes of chito-oligosaccharides.

Active Publication Date: 2017-06-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on the antidiabetic function of chitooligosaccharides are very limited.
At present, there are no literature and patent research reports on the anti-type II diabetes function of chitosan oligosaccharides

Method used

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  • Novel function of N-acetylchitobiose and application of N-acetylchitobiose in health care food
  • Novel function of N-acetylchitobiose and application of N-acetylchitobiose in health care food
  • Novel function of N-acetylchitobiose and application of N-acetylchitobiose in health care food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Alpha-Glucosidase Inhibitory Activity of Chitobiose

[0029] With acarbose (YY11290-1g, Biochemical reagent, Shanghai Yuanye Biotechnology Co., Ltd.) as a control, chitobiose (enzymatically prepared, Yang S, Fu X, Yan Q, et al. Cloning, expression, Purification and application of a novel chitinase from a thermophilic marine bacterium Paenibacillus barengoltzii[J].Food Chem,2016,192:1041-1048.) α-glucosidase inhibitory activity, method see (Rattanangkool et al.Quercitylcinnamates, a new series of antidiabetic bioconjugates possessing a-glucosidase inhibition and antioxidant. European Journal of Medicinal Chemistry, 2013, 66:296-304).

[0030] A brief description is as follows:

[0031]Take chitobiose with concentrations of 1, 2, 3, 4, 5, and 6 mg / mL, and acarbose with concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg / mL, respectively. 0.1U / mL α-glucosidase solution (α-glucosidase, EC 3.2.1.20, derived from Saccharomyces cerevisiae, cat.no.G5003-100UN, Si...

Embodiment 2

[0034] Example 2: Preventive and therapeutic effects of chitobiose on type II diabetic mice

[0035] 1. Materials and methods:

[0036] 1. Drugs and reagents

[0037] For acarbose and chitobiose, see Example 1 for their sources.

[0038] Streptozotocin, cat. no. V900890, Sigma-Aldrich, Inc., St. Louis, MO, USA.

[0039] Alloxan, cat. no. A7413, Sigma-Aldrich, Inc., St. Louis, MO, USA.

[0040] 2. Animal feeding and drug administration

[0041] ICR (Institute of Cancer Research) male mice weighing 18-20 g were randomly divided into the following groups:

[0042] 1. Healthy control group,

[0043] 2. Model control group,

[0044] 3. Positive control group (acarbose group),

[0045] 4. Chitobiose group, specifically including three dose groups: low dose 25mg / kg·d, medium dose 50mg / kg·d, high dose 100mg / kg·d,

[0046] There are 6 groups in total, 20 in each group.

[0047] The healthy control group was fed with common feed, and the rest of the groups were fed with high-fa...

Embodiment 3

[0093] Example 3: The hypoglycemic effect of compounding chitobiose and xylooligosaccharide

[0094] See Example 2 for the modeling method of diabetic mice. The compound group of chitobiose and xylooligosaccharide (Biochemical reagent, Shandong Longli Biotechnology Co., Ltd.) was divided into three groups: three doses of chitobiose (25, 50, 100mg / kg·d) respectively Compound 100mg / kg·d xylooligosaccharide (combination ratio: 1:4, 1:2, 1:1), with xylooligosaccharide (100mg / kg·d) and chitobiose (100mg / kg d) As a control, a healthy control group, a model control group and a positive control group (acarbose group) were simultaneously established, totaling 8 groups with 20 rats in each group.

[0095] The mice in each of the above groups were gavaged continuously for 4 weeks. During the test period, the mice drank water and ate food freely. The food intake was recorded every day, the body weight was recorded once a week, and the fasting blood sugar was measured. By comparing the f...

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PUM

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Abstract

The present invention concerns a novel function of N-acetylchitobiose and an application of N-acetylchitobiose in health care food. The N-acetylchitobiose is a degradation product of chitin, and is an oligosaccharide which is formed by the binding of two N-acetylglucosamine molecules through a glucosidic bond. Animal tests indicate that the N-acetylchitobiose has obvious inhibiting activity on alpha-glucosidase, and the IC50 of the N-acetylchitobiose is 2.0 mg / ml. The N-acetylchitobiose can significantly reduce the fasting glucose levels of mice with type II diabetes to a minimum value of 6.1 mg / ml. The minimum value of 6.1 mg / ml is significantly lower than the fasting glucose value of 7.9 mg / ml in an acarbose control group, and is close to the upper limit of a fasting blood glucose range in normal healthy mice. Secondly, compared to acarbose, the N-acetylchitobiose can significantly reduce serum blood lipid contents and inhibit the levels of lipid-peroxidation in pancreas. In addition, the N-acetylchitobiose can stabilize the weight of diabetic mice. The combination of the N-acetylchitobiose and xylo-oligosaccharide has obvious hypoglycemic effects as well. The N-acetylchitobiose provided by the invention has good hypoglycemic effects, and can be employed to develop safe and effective health care food which prevents and improves type II diabetes.

Description

technical field [0001] The invention relates to the field of health food, and specifically refers to the new function of chitobiose and its application in health food. Especially the function of chitobiose in preventing or improving type II diabetes and its application in health food. Background technique [0002] Chitin (Chitin, also known as chitin, chitosan or chitin) is a water-insoluble linear polymer formed by N-acetylglucosamine linked by β-1,4-glycosidic bonds. The content of renewable biomass is second only to cellulose, and the annual synthesis of chitin in the biological world is as high as tens of billions of tons. [0003] Chitin is widely found in the shells of shrimps, crabs, and insects, as well as in the cell walls of fungi, plants, and some green algae. Chitin can be degraded to generate chitooligosaccharides, such as the hydrolysis of β-1,4-glucosidic bonds in chitin chains to generate chitooligosaccharides (polysaccharides with a degree of polymerizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A61K31/7016A61K31/702A61P3/10
CPCA23V2002/00A61K9/0053A61K9/006A61K9/08A61K9/12A61K9/2059A61K9/4866A61K31/7016A61K31/702A61K47/46A23V2200/328A23V2200/3262A23V2250/60A61K2300/00
Inventor 江正强吴夏闫巧娟石玉琴王静
Owner CHINA AGRI UNIV
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