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Ferritin/chitosan-based natural pigment molecule embedding material preparation method

A natural pigment and ferritin technology, applied in the food field, can solve the problems of unreported and undisclosed use, and achieve the effects of improving water solubility, high practical application and promotion value, and improving bioavailability

Inactive Publication Date: 2017-06-16
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using soybean ferritin and chitosan as raw materials, using the reversible self-assembly properties of ferritin and protein-polysaccharide interaction to prepare uniformly dispersed, nano-scale ferritin-chitosan-natural pigment molecular embeddings There is no public report at home and abroad, and the present invention has no report at home and abroad, and there is no public use at home and abroad

Method used

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  • Ferritin/chitosan-based natural pigment molecule embedding material preparation method
  • Ferritin/chitosan-based natural pigment molecule embedding material preparation method
  • Ferritin/chitosan-based natural pigment molecule embedding material preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 takes lutein as an example

[0029] Accurately weigh 20 mg of lutein standard substance and dissolve it in 100 ml of absolute ethanol to prepare a mother solution with a concentration of 351.6 μM, and store it in the dark at 4°C until use. Put 5 mL of 2.0 μM ferritin solution (pH 7.8, Tris buffer) into a 10 mL centrifuge tube. Use 1M NaOH to adjust the pH value of the solution to about 11.0, stir at room temperature for 2 hours, slowly add 5.68mL lutein mother solution (stored in the dark at 4°C) to make the molecular molar ratio of ferritin:lutein 1:200, and stir at room temperature for 2 hours , to mix well. Then adjust the pH value of the solution to 7.5-8.0 with 1M HCl, and stir in a chromatographic cabinet at 4° C. for more than 2 h. The refolded solution was placed in a dialysis bag with a pore size of 1000 kDa, and dialyzed three times in a chromatographic cabinet at 4°C in the dark using Tris-HCl buffer (pH 7.5, 50 mM), and the dialysis buffer was...

Embodiment 2

[0030] Embodiment 2 takes rutin as an example

[0031]Accurately weigh 20 mg of standard rutin and dissolve it in 80% absolute ethanol to prepare a mother solution with a concentration of 323.0 μM, and store it in the dark at 4°C until use. Put 5 mL of 2.0 μM ferritin solution (pH 7.8, Tris buffer) into a 10 mL centrifuge tube. Use 1M NaOH to adjust the pH value of the solution to about 11.0. After stirring at room temperature for 2 h, slowly add 3.7 mL of rutin standard solution (stored in the dark at 4°C) to make the molecular molar ratio of ferritin:rutin 1:120, and stir at room temperature for 1 h. Let it mix well. Then adjust the pH value of the solution to 7.5-8.0 with 1M HCl, and stir in a chromatographic cabinet at 4° C. for more than 2 h. The refolded solution was placed in a dialysis bag with a pore size of 1000 kDa, and dialyzed three times in a chromatographic cabinet at 4°C in the dark using Tris-HCl buffer (pH 7.5, 50 mM), and the dialysis buffer was changed ev...

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Abstract

The invention discloses a novel method for preparing embeddings, which comprises the following steps: placing ferritin solution in a centrifuge tube, adjusting the pH value with sodium hydroxide solution to denature, and stirring; taking natural pigments and dissolving them in ethanol Solution: mix the polyphenol ethanol solution with the above-mentioned ferritin solution, stir at room temperature, adjust the pH value for renaturation, stir in a chromatographic cabinet at 4°C, and dialyze the above-mentioned solution three times to obtain the ferritin natural pigment embedding. Then, the ferritin pigment embedding material and chitosan were mixed in a certain proportion, stirred, statically prepared, and dialyzed in a chromatographic cabinet at 4°C in the dark to obtain the final product. The final product is a new type of embedding material loaded with natural pigment molecules by ferritin / chitosan, which can be used as a new food additive, can also be used to improve medical efficacy, and can also be used as a component in cosmetics, embedding material Ferritin and natural pigments produced after decomposition also have certain biological activity. This product makes full use of the functions of ferritin and natural pigment, and has high practical application and promotion value.

Description

technical field [0001] The invention discloses a preparation method of a ferritin / chitosan-based natural pigment molecule embedding, which belongs to the technology in the field of food. Background technique [0002] Ferritin is a kind of natural iron storage protein that widely exists in animals, plants and microorganisms. Due to its special hollow structure and unique protein shell, it is widely used as a carrier of metal ions and small organic molecules. The molecular structure of the ferritin shell is conservative, consisting of 24 subunits, and every two subunits form a group of antiparallel, forming an approximate regular octahedron, forming a hollow spherical molecule with 4-3-2 heavy axis symmetry, usually its inner diameter About 8nm, outer diameter about 12nm, thickness about 2nm. Generally speaking, the internal cavity of 8nm ferritin can store about 4500 ferric iron atoms to form a combined iron core. It can be seen that by using the reversible self-assembly p...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K47/42A61K47/36A61P3/02A23L33/10A61K8/73A61K8/64
Inventor 杨瑞高云晶周中凯孙国玉徐晶晶
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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