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Quick hypochlorite detection fluorescence probe, quick hypochlorite detection method and application thereof to tap water sample detection

A technology for detecting hypochlorous acid and fluorescent probes, applied in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve the problems of complex sensor synthesis, unfavorable practical application, etc., and achieve high selectivity.

Inactive Publication Date: 2017-06-13
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the synthesis of most sensors is relatively complex, which is not conducive to practical application

Method used

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  • Quick hypochlorite detection fluorescence probe, quick hypochlorite detection method and application thereof to tap water sample detection
  • Quick hypochlorite detection fluorescence probe, quick hypochlorite detection method and application thereof to tap water sample detection
  • Quick hypochlorite detection fluorescence probe, quick hypochlorite detection method and application thereof to tap water sample detection

Examples

Experimental program
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Embodiment 1

[0038] A fluorescent probe for rapid detection of hypochlorite, its preparation method is as follows:

[0039] 1) Prepare a HEPES (4-hydroxyethylpiperazineethanesulfonic acid) buffer solution with a concentration of 10 mM and a pH of 7.4; prepare CH of compound C1 3 CN solution, the concentration is 0.2mM; take 1mL of the above C1 CH 3 The CN solution was placed in a 10mL colorimetric tube, and the volume was constant with HEPES buffer solution to obtain a concentration of 20 μM in HEPES-CH 3 CN volume ratio 9:1 is the C1 solution of the solvent;

[0040] 2) Preparation of CH of CuI 3 CN solution, the concentration is 0.1M;

[0041] 3) prepare sodium ascorbate solution with deionized water, the concentration is 20mM;

[0042] 4) Add 3 μL concentration of 0.1M CuI solution and 1 μL concentration of 20mM sodium ascorbate aqueous solution (negligible impact on the total volume) to the solution of compound C1 obtained in step 1) to obtain a fluorescent probe for rapid detectio...

Embodiment 2

[0050] The fluorescent probe of the present invention is to the spectral response of common anion, and testing method comprises the steps:

[0051] 1) The composition, concentration and preparation method of the fluorescent probe are the same as in Example 1;

[0052] 2) Add 1 mmol of inorganic salt (NaClO, 680 μL; Na 2 SO 4 ,142mg; NaHSO 3 ,104mg; NaOAc·3H 2 O, 136 mg; NaClO 4 ,122 mg; Na 2 SO 3 ,126mg; NaF, 42mg; 2 CO 3 ,106mg; NaHCO 3 ,84mg; K 3 PO 4 ·3H 2 O, 266mg; NaCl, 58mg; KBr, 119mg; K 2 HPO 4 ·3H 2 O, 228 mg; NaIO 3 ,198mg; NaHSO 4 ,120mg; KClO 3 ,122mg; NaNO 2 ,69mg) and H 2 o 2 (100μL) was dissolved in 10mL deionized water to obtain common anion aqueous solutions with a concentration of 0.1M;

[0053] 3) Take 3mL of the fluorescent probe prepared in step 1) and place it in a quartz cuvette. Add the H prepared in step 2) with a volume of 1.0 μL 2 o 2 solution and a volume of 5.0 μL of SO prepared in step 2) 4 2- 、HSO 3 - 、AcO - , ClO 4 ...

Embodiment 3

[0056] The method for rapid detection of hypochlorite in tap water samples by fluorescent probes of the present invention mainly comprises the following steps:

[0057] 1) The composition, concentration and preparation method of the fluorescent probe are the same as in Example 1;

[0058] 2) Prepare a NaClO solution with a concentration of 0.1 mM with tap water;

[0059] 3) Take 3mL of the fluorescent probe solution prepared in step 1) and place it in a quartz cuvette. Add a volume of 200 μL of tap water, such as Figure 4 As shown, the fluorescence emission intensity was reduced to 70% of the original. according to figure 2 The linear equation of the standard curve can be calculated, and the concentration of hypochlorous acid in the tap water sample is about 1.8×10 -4 M.

[0060] 4) Take 3mL of the fluorescent probe solution prepared in step 1) and place it in a quartz cuvette. Add a volume of 200 μL tap water sample containing sodium hypochlorite, such as Figure 4 A...

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Abstract

The invention relates to a quick hypochlorite detection fluorescence probe, a quick hypochlorite detection method and application thereof to tap water sample detection. The fluorescence probe is mainly formed by coumarin derivative and cuprous salt mixed solution. The fluorescence probe used for detection of hypochlorite in aqueous solution is evident and quick in fluorescence quenching response and capable of realizing real-time hypochlorite detection; high detection sensitivity is realized, and a detection limit is as low as 1.4*10<-6>mol / L; according to a standard curve, the hypochlorite concentration of an actual tap water sample can be determined accurately; ultrahigh hypochlorite selectivity is realized while disturbance of other common anions and H2O2 is avoided.

Description

technical field [0001] The invention relates to a fluorescent probe and method for rapidly detecting hypochlorite and its application in tap water sample detection, belonging to the field of environmental protection. Background technique [0002] Hypochlorous acid (HClO) is a reactive oxygen species that plays an important role in our daily life and defense against host invasion by foreign pathogens. It is a strong oxidizing agent that kills bacteria in water and is widely used as a disinfectant for swimming pools. Hypochlorous acid can fade organic colors and dyes, so it can be used as a bleaching agent, oxidizing agent, deodorant and disinfectant. However, excessive hypochlorous acid in organisms can damage the host's own tissues and cause a series of diseases, such as kidney disease, arteriosclerosis and arthritis. Therefore, how to detect hypochlorous acid with high sensitivity and high selectivity is an urgent problem to be solved. [0003] At present, hypochlorous a...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 程晓红屈少华
Owner HUBEI UNIV OF ARTS & SCI
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