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Lactobacillus plantarum CH126 and application thereof in preparing functional food for reducing sugar

A kind of technology of Lactobacillus plantarum, CH126

Inactive Publication Date: 2017-06-13
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients with type 2 diabetes cannot effectively use insulin or form insulin resistance, which leads to a decrease in the utilization rate of glucose mediated by insulin, which in turn causes the metabolic system disorder of sugar, fat and protein in the body.
Clinical anti-diabetic drugs include insulin secretion promoting agents, biguanides, α-glucosidase inhibitors, thiazolidinediones, etc. Long-term use can cause visual abnormalities, gastrointestinal dysfunction, liver and kidney function damage, etc. side effects

Method used

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  • Lactobacillus plantarum CH126 and application thereof in preparing functional food for reducing sugar
  • Lactobacillus plantarum CH126 and application thereof in preparing functional food for reducing sugar
  • Lactobacillus plantarum CH126 and application thereof in preparing functional food for reducing sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, isolation, identification and preservation of plantarum lactobacillus strain

[0019] 1. Isolation of Lactobacillus plantarum strains

[0020] Sample material: traditional fermented food hot and sour radish.

[0021] After repeated streaking culture on the BCP medium plate, pick the single colony that produced the yellow circle and inoculate it on the solid MRS medium plate to continue the culture. Repeated streaking culture and purification on the solid MRS medium plate obtained multiple strains of pure culture strains.

[0022] The purely cultured strains were inoculated into liquid MRS medium for culture, then added 20% glycerol, and stored in a -80°C refrigerator.

[0023] From the purely cultured strains, some strains with positive Gram staining, negative catalase test, negative indole reaction, negative hydrogen sulfide production test, negative gelatin liquefaction test, negative hydrolyzed starch test and negative nitrate reduction test were screene...

Embodiment 2

[0031] Example 2, the functional properties of Lactobacillus plantarum CH126

[0032] The method of colony counting, after the completion of the culture, doubling dilution and spread on the solid MRS medium plate, 37 ℃ static culture for 48h after the colony counting.

[0033] 1. Acid resistance and bile salt resistance

[0034] 1. Acid resistance

[0035] Lactobacillus plantarum CH126 or Lactobacillus plantarum Sc52 were inoculated in liquid MRS medium with different pHs (adjusted to pH 2.0, 3.0 or 6.6 with 1 mol / L hydrochloric acid), cultured at 37°C for 1 hour or 3 hours, and then counted by colony counting Bacteria concentration (by Log 10 expressed in CFU / mL).

[0036] Three parallel treatments were set for each pH, ​​and three parallel treatments were set again for each dilution, and the results were averaged.

[0037] The results are shown in Table 1. Lactobacillus plantarum CH126: The strain grew well under the condition of pH 6.6, and the number of viable bacteri...

Embodiment 3

[0085] Example 3, Regulatory Effect of Lactobacillus plantarum CH126 on Blood Sugar

[0086] 1. Group processing method

[0087] 80 male C57BL / 6J mice were randomly divided into 4 groups, 20 mice in each group, and the grouping treatment was as follows:

[0088] Blank control group: fed with common feed from the beginning to the end of the test; at the ninth week, the mice were fasted for 12 hours and injected with 30 mg / kg of normal saline intraperitoneally; "Administer 12 mL of sterile PBS buffer per kilogram of body weight each time";

[0089] Model group: fed with high-fat feed from week 1 to week 8, and fed with common feed from week 9 to the end of the experiment; at week 9, mice were given intraperitoneal injection of streptozotocin 30 mg / kg after 12 hours of fasting; Dosing from the 10th week to the 13th week; the dosage is "12mL sterile PBS buffer per kilogram of body weight";

[0090] Positive control group: fed with high-fat feed from week 1 to week 8, and fed wi...

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Abstract

The invention discloses a lactobacillus plantarum CH126 and an application thereof in preparing functional food for reducing sugar. The Lactobacillus plantarum CH126 provided by the invention is called as lactobacillus plantarum CH126 for short, with the preserving register number: CGMCC NO. 13228. The invention also provides the application of the lactobacillus plantarum CH126 in preparing products. The products include food, health care products or drugs. The food is functional food, such as, functional yoghourt and functional fungicide. The function of the products is to reduce blood sugar and / or blood fat and to treat and / or prevent diabetes mellitus. The lactobacillus plantarum CH126 has strong functions of reducing fasting blood-glucose and reducing blood fat and can be widely applied to the fields of food, healthcare products and medicinal health.

Description

technical field [0001] The invention relates to plant lactobacillus CH126 and its application in preparing hypoglycemic functional food. Background technique [0002] Diabetes Mellitus (DM) is a common chronic metabolic disease, often accompanied by atherosclerosis, nephropathy, neuropathy, retinopathy and other complications. Patients with type 2 diabetes cannot effectively use insulin or form insulin resistance, which leads to a decrease in the utilization rate of glucose mediated by insulin, which in turn causes metabolic disorders of sugar, fat, and protein in the body. Clinical anti-diabetic drugs include insulin secretion promoting agents, biguanides, α-glucosidase inhibitors, thiazolidinediones, etc. Long-term use can cause visual abnormalities, gastrointestinal dysfunction, liver and kidney function damage, etc. side effects. Therefore, it is more practical to find a safe, reliable, and side-effect-free method for lowering blood sugar. [0003] Probiotics are live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/10A61P3/06A23L33/135C12R1/25
CPCA23V2002/00A61K2035/115C12N1/205C12R2001/25A23V2200/328
Inventor 李盛钰高磊段翠翠赵玉娟牛春华栾畅
Owner JILIN ACAD OF AGRI SCI
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