Preparation method of semen plantaginis polysaccharide extract

Abstract

The invention provides a preparation method of a semen plantaginis polysaccharide extract. The preparation method comprises the following steps: (1) raw material treatment, namely drying semen plantaginis, removing impurities, crushing, breaking hulls, adding petroleum ether, and carrying out reflux degreasing, so as to obtain residues; (2) rough extraction, namely adding distilled water with the weight of 6-12 times of weight of the residues and a cosolvent with the weight of 0.1-0.2 time of weight of the residues, carrying out ultrasonic assistant dissolving and concentration, adding ethanol, and carrying out alcohol precipitation and centrifugation, so as to obtain a precipitate, sequentially and respectively washing the precipitate with absolute ethyl alcohol and acetone, dissolving the precipitates with distilled water, and carrying out dialysis, concentration and freeze drying, so as to obtain a precipitate A; and (3) extraction, namely sequentially and respectively washing the precipitate A with absolute ethyl alcohol and acetone, dissolving the precipitate A with distilled water while heating, carrying out centrifugation, extracting supernate, removing color matters and proteins, and carrying out reduced pressure concentration, so as to obtain the semen plantaginis extract. According to the preparation method, the semen plantaginis is subjected to hull-breaking pretreatment and is subjected to secondary extraction through assistant dissolving by virtue of the cosolvent and ultrasonic waves, and the prepared extract is high in polysaccharide content, and the extraction process is simple.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a preparation method of plantago polysaccharide extract. Background technique [0002] Active ingredients with anti-complement effects widely exist in natural medicines. Some natural medicines such as ephedra, ginseng, and Eucommia have been screened at home and abroad, and some polysaccharides, proteins, polypeptides, steroids, terpenes, and Chemicals such as alkaloids have significant anti-complement activities, among which glycosylated proteins and polysaccharides are the most common. Heparin is a sulfated polysaccharide polymerized by uronic acid and glucosamine. Mature complement inhibitor, but because heparin has an anticoagulant effect, it needs a higher concentration in the body to exert its drug effect, and its side effects are large, which limits its clinical use. [0003] Plantago is one of the authentic medicinal materials widely used i...

AI Technical Summary

Problems solved by technology

[0004] Existing technologies such as Chinese Invention Authorized Patent Documents, Authorized Announcement No. CN 102138981 B This invention relates to a preparation method and medical use of an immunosuppressive active plantain seed extract. The crude extract is obtained by precipitation, and the crude extract undergoes dialysis and ion exchange chromatography to remove proteins and pigments, remove small molecular chemical components, and make a higher-purity Plantago extract, the immunosuppressive active Plantago in this invention Extract, wherein the total polysaccharide content accounts for more than 72% of the weight percentage of the extract. The invention has found through a large number of pharmacological experiments that the plantago seed extract has immunosuppressive activity, and can be used as an immunosuppressant or immunosuppressant in various dosage forms. As a raw material for the preparation of other immunosuppressive drugs, it is used to treat diseases related to immune inflammatory damage: nephritis, cystitis, prostatitis, urethritis, edema, uveitis, keratitis, corneal transplantation rejection The medical use of eye diseases, etc., but the invention is lacking in the pre-treatment of medicinal materials. For example, the extraction rate may be affected if the shell is not broken. There is still room for improvement in the extraction of psyllium polysaccharides.