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Method for solid-phase extraction of phosphatidylcholine in Antarctic krill in 96-well plate

A technology for phosphatidylcholine and Antarctic krill, which is applied in the field of extracting phosphatidylcholine from Antarctic krill, can solve the problems of low processing and utilization of Antarctic krill, low product quality, and low extraction efficiency, and achieves simple training. , high phospholipid purity, good extraction effect

Active Publication Date: 2019-03-26
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for solid-phase extraction of phosphatidylcholine in Antarctic krill using a 96-well plate; the method effectively combines liquid-liquid extraction, 96-well plate, and solid-phase extraction to extract Antarctic krill with high purity. Phosphatidylcholine in krill has a high throughput and can extract 96 samples at the same time, thereby solving the existing problems of low extraction efficiency, poor specificity, low product quality, and low degree of processing and utilization of Antarctic krill And other issues

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  • Method for solid-phase extraction of phosphatidylcholine in Antarctic krill in 96-well plate
  • Method for solid-phase extraction of phosphatidylcholine in Antarctic krill in 96-well plate
  • Method for solid-phase extraction of phosphatidylcholine in Antarctic krill in 96-well plate

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Embodiment 1

[0054] Example 1. A method for solid phase extraction of phosphatidylcholine from Antarctic krill in a 96-well plate, followed by the following steps:

[0055] 1). Raw material pretreatment:

[0056] Take fresh Antarctic krill, remove the head and shell to obtain muscle tissue, wash and grind into minced meat, vacuum dry to moisture content ≤5% (wt%), pulverize into powder (can pass through 80-100 mesh sieve) to obtain Antarctic krill powder.

[0057] 2) Preparation of crude extract (liquid-liquid extraction):

[0058] Precisely weigh 0.1g of ground Antarctic krill powder, extract with 3 mL of chloroform-methanol (2:1, volume ratio) mixture with ice bath ultrasonic (0℃, power 200W) for 30 min, add 1.5 mL of ultrapure water and 12,000 rpm / min high-speed freezing (ie, 4°C) centrifugation for 5 minutes. Use a 200 mL pipette to transfer the subnatant, add 2 mL of chloroform to the remaining supernatant and solids, and repeat the extraction twice according to the above operation. The th...

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Abstract

The invention discloses a method for solid phase extraction of phosphatidylcholine in euphausia superb by virtue of a 96-pore plate. The method comprises the following steps: preprocessing muscular tissues of the euphausia superb, so as to obtain euphausia superb powder; adding an organic solvent I into the euphausia superb powder, uniformly mixing, carrying out ice-bath ultrasonic extraction, adding water, carrying out oscillation centrifugation, and extracting subnatant after the solution is layered; carrying out reduced pressure drying on the subnatant, and redissolving the subnatant by virtue of an organic solvent II, so as to obtain euphausia superb phospholipid crude extraction liquid; activating a 96-pore solid phase extraction plate by virtue of an organic solvent III water solution, and carrying out euphausia superb phospholipid crude extraction liquid sample feeding, organic solvent III water solution elution, organic solvent IV or organic solvent IV water solution elution, so as to obtain a purified phosphatidylcholine extract.

Description

Technical field [0001] The present invention relates to the technical field of material separation, in particular to a method for extracting phosphatidylcholine from Antarctic krill. Background technique [0002] Antarctic krill (Euphausia superba) is a type of crustacean zooplankton that lives in the Southern Ocean. It has the characteristics of large biological reserves and wide distribution, and is an important strategic new marine resource. It is estimated that the Antarctic krill reserves are as large as 650 to 1 billion tons, and the maximum tolerable catch is about 4 to 6 million tons. Antarctic krill is an important part of the Antarctic sea ecosystem and has received extensive attention in recent years. Scientific research institutions of various countries have carried out the deep processing technology of Antarctic krill, and conducted a lot of research in the fields of food, medicine, and health products, which is one of the hot research topics in recent years. Studi...

Claims

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Application Information

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IPC IPC(8): C07F9/10B01D11/02
CPCB01D11/0219C07F9/103
Inventor 沈清戴志远
Owner ZHEJIANG GONGSHANG UNIVERSITY
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