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A kind of preparation method of chitosan-alginate porous ball hemostatic material

A technology of alginate and hemostatic material, which can be used in pharmaceutical formulations, surgery, drug delivery, etc., can solve the problems of wound tissue damage, limit wide application, etc., and achieve high porosity, high-efficiency hemostatic activity, and good biocompatibility. Effect

Active Publication Date: 2019-05-21
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among the hemostatic material products, U.S. Celox hemostatic powder and HemCon hemostatic bandage mainly use chitosan as a hemostatic agent. The British Courtaulds company applied the alginate fiber dressing named Sorbsan to the field of hemostasis in the 1980s, but There are some critical bottleneck issues, especially the hemostasis of wounds such as irregular shape, deep, narrow, ruptured arteries, which limit their wide application
In 2002, QuikClot zeolite hemostatic powder was approved by the FDA for emergency treatment of severe bleeding wounds. However, in use, QuikClot zeolite hemostatic powder has the defect of exothermic reaction exceeding 100°C, causing wound tissue damage
WoundStat hemostatic agent was approved by the US FDA in 2008 and was widely used in the military in the same year, but it was later found that there was a risk of peripheral thrombus entering the blood circulation system. Based on biosafety considerations, the US military announced in 2009 that it would ban the permanent use of WoundStat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] S1. Dissolve chitosan (molecular weight 250 kDa, deacetylation degree 90%, carboxymethyl substitution degree 50%) with purified water to prepare 3% (W / V) chitosan aqueous solution; dissolve sodium alginate ( Viscosity 200mPa s), prepare 3% (W / V) sodium alginate aqueous solution; take equal volumes of the above two solutions and mix well to prepare chitosan-sodium alginate composite aqueous solution;

[0020] S2. Add the cross-linking agent calcium acetylacetonate to the isopropanol-isooctanol (volume ratio 4:6) compound alcohol solution (the content of the cross-linking agent in the compound alcohol solution is 1%, W / V), and place it in the ultrasonic Ultrasound in the instrument; drop the above-mentioned chitosan-sodium alginate composite aqueous solution into the complex alcohol solution with a constant pressure dropping funnel (the volume ratio of the composite aqueous solution to the complex alcohol solution is 1:8), and the dropping speed is 20 drops / min. After the...

Embodiment 2

[0024] S1. Dissolve chitosan (molecular weight 180 kDa, deacetylation degree 93%, carboxymethyl substitution degree 65%) with purified water to prepare 3.5% (W / V) chitosan aqueous solution; dissolve sodium alginate ( Viscosity 400 mPa s), prepare 2% (W / V) sodium alginate aqueous solution; take equal volumes of the above two solutions and mix well to prepare chitosan-sodium alginate composite aqueous solution;

[0025] S2. Add the cross-linking agent calcium acetylacetonate to the isopropanol / isooctanol (volume ratio 4:6) complex alcohol solution (the content of the cross-linking agent in the complex alcohol solution is 1.5%, W / V), and place it in the ultrasonic Ultrasound in the instrument; drop the above chitosan-sodium alginate composite aqueous solution into the complex alcohol solution with a constant pressure dropping funnel (the volume ratio of the composite aqueous solution to the complex alcohol solution is 1:8), and the dropping speed is 24 drops / min. After the additi...

Embodiment 3

[0029] S1. Dissolve chitosan (molecular weight 100 kDa, deacetylation degree 93%, carboxymethyl substitution degree 70%) with purified water to prepare 5% (W / V) chitosan aqueous solution; dissolve potassium alginate ( Viscosity 600 mPa s), prepare 2% (W / V) potassium alginate aqueous solution; take equal volumes of the above two solutions and mix well to prepare chitosan-potassium alginate composite aqueous solution;

[0030] S2. Add the cross-linking agent calcium acetylacetonate to the isopropanol / isooctanol (volume ratio 4:6) complex alcohol solution (the content of the cross-linking agent in the complex alcohol solution is 2%, W / V), and place it in the ultrasonic Ultrasound in the instrument; drop the above-mentioned chitosan-potassium alginate composite aqueous solution into the composite alcohol solution with a constant pressure dropping funnel (the volume ratio of the composite aqueous solution to the composite alcohol solution is 1:7), and the dropping speed is 26 drops / ...

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Abstract

The invention discloses a preparation method of a chitosan-alginate porous ball hemostatic material. The method comprises the following steps of: S1, preparing a chitosan-alginate compound aqueous solution; S2, adding a crosslinking agent, namely calcium acetylacetonate, into a compound alcohol solution, dropwise adding the chitosan-alginate compound aqueous solution into the compound alcohol solution under an ultrasonic condition at the dropwise adding speed of 20-30 drops / min, after dropwise adding, stopping ultrasonic treatment, and stirring the mixture for 30min at 500-1000rpm; and S3, filtering balls, and washing, freezing and drying the balls. The hemostatic material prepared by the preparation method is good in biocompatibility, is safe and non-toxic, can quickly accelerate blood clotting, has efficient hemostatic activity, has a certain volume, and is unlikely to enter blood to induce a thrombus risk. Meanwhile, the material has excellent wound adaptability, and is particularly suitable for bleed stopping of wounds which are deep, narrow and irregular. The preparation process is simple and easy for industrial production.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a method for preparing a chitosan-alginate porous ball hemostatic material. Background technique [0002] In times of war, more than 80% of casualties are loss of life due to traumatic blood loss; in peaceful times, massive blood loss due to trauma caused by natural disasters, accidents and other emergencies is also the main cause of disability and death. Traditional hemostatic cotton gauze, bandages, etc. have unsatisfactory hemostatic effects on irregularly shaped, deep, narrow, ruptured arteries and other wounds; although hemostatic powder has excellent wound applicability, it is easy to enter the blood and block the flow of peripheral arteries to form thrombus. Therefore, a fast, safe and efficient hemostatic material suitable for on-site and clinical first aid is needed to replace traditional hemostatic materials. [0003] Chitosan is a natural biological polysaccharide ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/001A61L24/0036A61L24/08A61L2400/04C08L5/08C08L5/04
Inventor 胡章李思东洪鹏志廖铭能孔松芝程瑜黄娜
Owner GUANGDONG OCEAN UNIVERSITY
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