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Preparation and application of microcystin-LR quantitative and rapid detection sensor

A technology for detecting sensors and microcystins, applied in the biological field, can solve the problems of time-consuming and labor-intensive, unable to monitor real-time source water, etc., and achieve the effect of sensitive detection

Inactive Publication Date: 2017-05-31
CHONGQING DINGSHENG BIOTECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, these methods are time-consuming and laborious, have hysteresis, and cannot monitor the source water in real time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Quantitative and rapid detection of microcystin-LR in natural lake water:

[0030] 1 Pre-processing:

[0031] Sampling was carried out by a plexiglass deep-water sampler, the water sampling depth was below 0.5m, and the sampling volume was 5L. Remove large particles of impurities through a sand core funnel, and then filter under reduced pressure through a 0.45 μm filter membrane. Collect the filtrate. Take an appropriate amount of filtrate for detection and analysis.

[0032] 2. Microcystin-LR quantitative rapid detection sensor usage method:

[0033] Take out the prepared MC-LR immune electrode sensor and equilibrate to room temperature. In order of concentration from low to high, 10 μL of MC-LR standard solution and sample extract of different concentrations were added dropwise on the surface of the electrode, incubated at 37°C for 15 min, washed with PBS and dried. Using cyclic voltammetry (CV), alternating current impedance method (EIS) in [F...

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PUM

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Abstract

The invention relates to preparation and application of a microcystin-LR quantitative and rapid detection sensor, and belongs to the technical field of biology. The detection sensor is an impedance type electrochemical sensor and consists of a CHI760C type electrochemical working station and three-electrode system, wherein the three-electrode system comprises an immunoelectrode serving as a working electrode, a saturated calomel electrode serving as a reference electrode and a platinum wire electrode serving as a counter electrode; an L-cysteine, colloidal gold and microcystin antibody composition is wrapped on the surface of a bare gold electrode to prepare the working electrode; when a microcystin antibody is combined with an antigen (MC-LR) in sample liquid, a change of a potential difference between the reference electrode and the working electrode is caused; the change and the antigen concentration of a sample are in a quantitative relation, so as to carry out detection of microcystins; a fast, specific and sensitive biosensor for detecting a microcystin antigen is successfully constructed; the detection linear range is 0.05 to 300 ng / mL, and the detection limit is 1.82*10<-2> ng / mL. The microcystin-LR quantitative and rapid detection sensor can monitor natural lake water in real time and also monitor a preparation process of drinking water for a water plant in real time.

Description

technical field [0001] The preparation and application of a microcystin-LR quantitative rapid detection sensor belongs to the field of biotechnology. Background technique [0002] Microcystins (MC for short) have become one of the pollutants that are widely concerned in environmental water. MC is a kind of biologically active cyclic heptapeptide compound, which is mainly produced by the freshwater alga Microcystis aeruginosa. So far, more than 70 isomers of MC have been found. It is a highly specific toxin with the liver as the target organ, and has obvious hepatotoxicity. With the in-depth study of MC toxicity, it is found that MC has multi-organ toxicity, hereditary and carcinogenicity. The structure of algal toxins is mainly composed of N-methyl dehydroalanine and two L-amino acid residues X and Z. Common three toxins are MC-LR, RR, and YR (L, R, and Y represent leucine respectively Acid, arginine, tyrosine), and MC-LR is the main toxin in fresh water. Algae toxin is ...

Claims

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Application Information

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IPC IPC(8): G01N33/561
CPCG01N33/561
Inventor 谢兵秦检
Owner CHONGQING DINGSHENG BIOTECH CO LTD
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