Primer for real-time fluorescent quantitative PCR rapid detection of vibrio parahaemolyticus toxR gene of marine product and kit of primer

Abstract

The invention discloses a primer for real-time fluorescent quantitative PCR (polymerase chain reaction) rapid detection of a vibrio parahaemolyticus toxR gene of a marine product. The primer comprises a sense primer toxR-F, an antisense primer toxR-R, and an amplification product 130bp. Accordingly, the inventor also assembles a corresponding detection kit and establishes a corresponding detection method. A specificity test, a sensitivity test and a repeatability test prove that the primer provided by the invention has good specificity, high sensitivity, good stability, and a short detection time, and is an effective method for rapid detection of vibrio parahaemolyticus. Results of the application of the kit provided by the invention in a repeatability test of a same sample and in tests of different batches of the same sample show that the method can be used for detecting the vibrio parahaemolyticus in a large quantity of basic-level marine products, and an established method for fluorescent quantitative PCR rapid detection of the vibrio parahaemolyticus is good in repeatability, and can be used for performing stable and reliable detection on the vibrio parahaemolyticus in marine products.

Description

technical field [0001] The invention belongs to the technical field of vibrio parahaemolyticus detection, in particular to a real-time fluorescent quantitative PCR rapid detection primer and a kit for the toxR gene of vibrio parahaemolyticus in seafood. Background technique [0002] Vibrio parahaemolyticus (VP) is a Gram-negative halophilic bacterium that grows in harbors and estuaries. It can be enriched in organisms through the filter-feeding activities of aquatic animals, so it widely exists in a variety of seafood. Among them, including fish, crustaceans, bivalve molluscs, etc. Among them, oysters have the highest content. Human consumption of seafood contaminated with this bacteria can cause abdominal pain, diarrhea, and in severe cases, sepsis, shock, coma and death. In recent years, food poisoning caused by Vibrio parahaemolyticus has broken out globally, especially in food poisoning cases in coastal areas of China. Vibrio parahaemolyticus has become the primary pat...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of Vibrio parahaemolyticus in food are mainly based on national standards and industry standards. These methods have a long inspection cycle and are subject to more subjective influences, which cannot meet the needs of a food hygiene rapid response system.

In recent years, PCR technology has been widely used in this field. In the conventional PCR method, the tube opening operation is easy to be polluted, causing false positive defects.

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