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Chinese herbal medicine compound micro-ecology preparation and preparation method and use thereof

A technology of composite microecology and Chinese herbal medicine, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to stimulate the immunity of aquatic animals, single efficacy of microecological preparations, and inability to improve water quality, etc. Achieve the effects of improving feed utilization and conversion rate, inhibiting reproduction and growth, and improving immunity

Active Publication Date: 2017-05-31
清远海贝生物技术有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on microecological preparations in the prior art can either be used to improve water quality, or can be used to improve the immunity of aquatic animals, and the microecological preparations used to improve the immunity of aquatic animals basically rely on adding them to the feed. The way of feeding, for example, discloses " multi-functional sea cucumber cultivation compound microecological preparation " in the open text of patent application number CN201010602297.0, and this microecological preparation adopts Bacillus atrophaeus, Bacillus subtilis and Bacillus cereus, The preparation is added to the feed for feeding, which has antibacterial, immune, growth-promoting and other effects, but does not have the effect of improving water quality; the publication of the patent application number CN20140461391.7 discloses "a compound microecological preparation and its preparation method", the micro-ecological preparation adopts Bacillus subtilis, Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus licheniformis, Enterococcus faecium, Lactobacillus pumilus, Lactobacillus plantarum, Lactococcus lactis and Saccharomyces cerevisiae, etc., can be used It is used to degrade harmful substances such as ammonia nitrogen and hydrogen sulfide in the water body to improve water quality, but it cannot stimulate the immunity of aquatic animals; in the publication of the patent application number CN201310210210.09, "a Chinese herbal medicine feed additive for improving the immunity of aquatic animals" is disclosed. This Chinese herbal medicine additive is used by adding it to the feed. This additive can only promote the immunity of aquatic animals, and has no effect on improving the water quality of aquaculture.
These microecological preparations have a single effect, and the method of use is also limited, it is not easy to popularize and use, and the economic value is limited

Method used

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  • Chinese herbal medicine compound micro-ecology preparation and preparation method and use thereof

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preparation example Construction

[0047] As a preference of the present invention, step (1) in the above-mentioned preparation method includes: pulverizing and mixing the raw materials of Chinese herbal extracts including astragalus, wormwood, cassia seed, angelica and eucommia ulmoides, adding 3-11 times (for example, 4 Times) volume of water soaked for 8-10h, decocted at 100-110℃ for 1-2 hours (for example, 2 hours), separated and filtered to obtain filter residue and filtrate, add 3-4 times volume of water to the filter residue (for example, 4 times volume Water), and then decoct at 100~110℃ for 1-2 hours (for example, 1 hour), and then filter to separate to obtain the filtrate of the first repeated decoction. Add 3-4 times the volume of water (for example, 3 times the volume of water) to the filter residue after repeated decocting. After decocting for 0.5-1 hour (for example, 0.5 hour) at 100-110°C, separate and filter to obtain the second repeat The decoction filtrate is mixed with the filtrate, the filtra...

Embodiment 1

[0081] The preparation of a Chinese herbal medicine compound microecological preparation for aquaculture, the specific steps are as follows:

[0082] (1) Preparation of Chinese herbal medicine extract: 30-35% astragalus, 10-15% mugwort leaf, 15-20% cassia seed, 15-20% angelica, 10-15% eucommia as raw materials, after the raw materials are crushed and mixed evenly, add Soak in 4 times water for 9h, decoct at 105℃ for 2h, separate and filter, add 4 times of water and then decoc for 1h, filter again, separate filter residue, add 3 times of water, decoc for 0.5h, separate and filter, and mix the filtrate from three times of filtration , Which is the Chinese herbal medicine extract;

[0083] (2) Preparation of compound bacterial liquid:

[0084] ① Strain activation and rejuvenation: The slant strains of Lactobacillus, Bacillus subtilis, yeast, and photosynthetic bacteria are respectively inoculated with an inoculation loop under aseptic conditions to contain liquid that has been steriliz...

Embodiment 2

[0092] The preparation of a Chinese herbal medicine compound microecological preparation for aquaculture, except that the proportion of Chinese herbal medicines is changed, the other preparation steps are the same as in Example 1, and the number of viable cells of the Chinese herbal medicine compound microecological preparation is 1.6×10 9 cfu / mL.

[0093] The raw material components of the Chinese herbal medicine extract in terms of mass percentage are 30% astragalus, 20% mugwort, 20% cassia, 15% angelica, and 15% eucommia.

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Abstract

The invention relates to a Chinese herbal medicine compound micro-ecology preparation and a preparation method thereof, and a use in the aquaculture. The micro-ecology preparation is mainly prepared by Chinese herbal medicine extracting solution, a fermentation medium, and compound bacteria solution through the fermentation. The Chinese herbal medicine extracting solution is mainly extracted from 30-35% of astragalus membranaceus, 10-15% of folium artemisiae argyi, 15-20% of semen cassia, 15-20% of Angelica sinensis, and 10-15% of eucommia ulmoides. The compound bacteria solution is prepared by fermenting and mixing lactobacillus, Bacillus subtillis, saccharomycetes and photosynthetic bacteria. The Chinese herbal medicine compound micro-ecology preparation is capable of purifying water quality and improving aquatic livestock immunity in the aquaculture process, rapidly reducing the hazardous substance in the water body, improving the water quality, inhibiting the breeding and growth of pathogenic microorganism, stimulating the own immunity and anti-stress capacity of the aquatic livestock, reducing the occurrence of diseases, improving the survival rate of the aquatic livestock, reducing the death rate, and improving the utilization rate and the conversation rate of feed.

Description

Technical field [0001] The invention relates to a Chinese herbal medicine compound microecological preparation, a preparation method thereof, and use in aquaculture. Background technique [0002] In recent years, the demand for aquatic animals has continued to increase, and fishing alone is far from being able to meet market demand. Therefore, the rapid development of the aquatic animal artificial breeding industry has been born. [0003] China is currently the world's largest consumer of aquaculture. With the advancement of aquaculture technology, the large-scale aquaculture industry has developed rapidly and brought more and more economic value. However, while large-scale aquaculture is developing, There is also the problem of deterioration of the water environment and frequent diseases. At the same time, the abuse of antibiotics and the use of other drugs have caused the normal microecological flora of aquatic animals’ gastrointestinal flora to be imbalanced, and the development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34A23K10/18C12R1/225C12R1/125
CPCC02F3/34C02F3/347C12N1/14C12N1/20
Inventor 王凯王蔚淼齐振雄
Owner 清远海贝生物技术有限公司
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