Rapid detection kit for nuclear matrix protein 22 and application thereof

A nuclear matrix protein and detection kit technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to meet clinical requirements, poor stability of chemiluminescent immunoassay kits, etc., and achieve the benefit of early Diagnosis and prognostic treatment, improvement of sensitivity and accuracy, high sensitivity effect

Active Publication Date: 2017-05-17
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nuclear matrix protein 22 chemiluminescence immunoassay detection kit has poor stability and cannot meet clinical requirements

Method used

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  • Rapid detection kit for nuclear matrix protein 22 and application thereof
  • Rapid detection kit for nuclear matrix protein 22 and application thereof
  • Rapid detection kit for nuclear matrix protein 22 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, a kind of rapid detection kit of nuclear matrix protein 22

[0036] The preparation of the rapid detection kit of nuclear matrix protein 22

[0037] 1. Preparation of solid-phase carrier coated with nuclear matrix protein 22 monoclonal antibody:

[0038] The nuclear matrix protein 22 monoclonal antibody was added into a sodium citrate buffer with a pH of 4.2 to make a coating solution. The added amount of the nuclear matrix protein 22 monoclonal antibody was 0.8% of the volume of the sodium citrate buffer. Add the above coating solution into a 96-well microwell plate, 100 μL per well, place at 4°C overnight, shake off the coating solution, then add 300 μL of blocking solution to each well, place at room temperature for 3-5 hours, shake off the blocking solution, and dehumidify at room temperature Dry for 24 hours to obtain;

[0039] The blocking solution is composed of the following components: sodium citrate buffer 30g / L, tetradecyltrimethylammonium chl...

Embodiment 2

[0047] Embodiment 2, a kind of rapid detection kit of nuclear matrix protein 22

[0048] The preparation of the rapid detection kit of nuclear matrix protein 22

[0049] 1. Preparation of solid-phase carrier coated with nuclear matrix protein 22 monoclonal antibody:

[0050] The nuclear matrix protein 22 monoclonal antibody was added into a sodium citrate buffer solution with a pH of 4.2 to make a coating solution, and the addition amount of the nuclear matrix protein 22 monoclonal antibody was 1.0% of the volume of the sodium citrate buffer solution. Add the above coating solution into a 96-well microwell plate, 100 μL per well, place at 4°C overnight, shake off the coating solution, then add 300 μL of blocking solution to each well, place at room temperature for 3-5 hours, shake off the blocking solution, and dehumidify at room temperature Dry for 24 hours to obtain;

[0051] The blocking solution consists of the following components:

[0052] Sodium citrate buffer 45g / L,...

Embodiment 3

[0060] Embodiment 3, a kind of rapid detection kit of nuclear matrix protein 22

[0061] The preparation of the rapid detection kit of nuclear matrix protein 22

[0062] 1. Preparation of solid-phase carrier coated with nuclear matrix protein 22 monoclonal antibody:

[0063] The nuclear matrix protein 22 monoclonal antibody was added into a sodium citrate buffer solution with a pH of 4.2 to make a coating solution, and the addition amount of the nuclear matrix protein 22 monoclonal antibody was 1.2% of the volume of the sodium citrate buffer solution. Add the above coating solution into a 96-well microwell plate, 100 μL per well, place at 4°C overnight, shake off the coating solution, then add 300 μL of blocking solution to each well, place at room temperature for 3-5 hours, shake off the blocking solution, and dehumidify at room temperature Dry for 24 hours to obtain;

[0064] The blocking solution consists of the following components:

[0065] Sodium citrate buffer 50g / L,...

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Abstract

The invention belongs to the technical field of biological detection, and specifically relates to a rapid detection kit for nuclear matrix protein 22 and application thereof. The rapid detection kit for the nuclear matrix protein 22 disclosed by the invention mainly consists of a solid-phase carrier enveloped by a monoclonal antibody to the nuclear matrix protein 22, the monoclonal antibody to the nuclear matrix protein 22 labelled by horseradish peroxidase, acridinium ester, washing liquid and nuclear matrix protein 22 calibrator. The rapid detection kit for the nuclear matrix protein 22 disclosed by the invention can detect an expression level of the nuclear matrix protein 22 in body fluid of a patient after surgical treatment or recurrent and metastatic tumor patient, and has an important meaning for clinical diagnosis of bladder cancer; and the rapid detection kit for the nuclear matrix protein 22 disclosed by the invention has the advantages of high accuracy and stability in a detection result and good sensitivity, and is favorable for early diagnosis and prognosis treatment of the bladder cancer.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a rapid detection kit for nuclear matrix protein 22 and an application thereof. Background technique [0002] Bladder tumors are the most common tumors in the urinary system, and more than 90% are transitional cell carcinomas. Bladder transitional cell carcinomas (transitional cell carcinoma of bladder) mostly occur between the ages of 50 and 70, and more than 80% of them are superficial tumors. % to 20% develop invasive bladder cancer. At present, the means of bladder cancer diagnosis and postoperative review mainly include cystoscopy and urine cytology examination. Although cystoscopy is accurate, it is an invasive examination. Pain makes many patients resist this method, and it is also difficult to find carcinoma in situ or squamous carcinoma. Although urine cytology is non-invasive and has good specificity, its sensitivity is low. For tumors with lower stages...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57407G01N33/57484G01N33/6893
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner ANHUI IPROCOM BIOTECH CO LTD
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