Pineapple ferment and application of pineapple ferment as tyrosinase inhibitor

A pineapple enzyme and pineapple technology, applied in skin care preparations, cosmetic preparations, dressing preparations, etc., can solve the problems of resource waste of pineapple peels, environmental pollution, etc., to solve resource waste, raw materials are cheap and easy to obtain, and restrain The effect of tyrosinase

Active Publication Date: 2017-05-17
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The pineapple ferment provided by the invention relies on the wild yeast or bromelain on the surface of the pineapple peel or pineapple leaf, and is obtained by natural fermentation in the presence of glucose. The by-product of pineapple processing—the wast

Method used

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  • Pineapple ferment and application of pineapple ferment as tyrosinase inhibitor
  • Pineapple ferment and application of pineapple ferment as tyrosinase inhibitor
  • Pineapple ferment and application of pineapple ferment as tyrosinase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Tyrosinase Promotes the Determination of the Best Activity Conditions for L-Tyrosine to Generate Melanin

[0027] (1) Effect of temperature on the optimal activity of tyrosinase

[0028] Take 8 clean and dry brown volumetric flasks of 10mL and divide them into 4 groups, 2 in each group, and mark them as C1 and C2 respectively. Add each test solution according to Table 1:

[0029] Table 1 System composition and the addition amount of each reagent

[0030]

[0031] Add the corresponding amount of buffer solution and L-tyrosine solution to the 4 sets of volumetric flasks according to the above table as the substrate of the reaction system, then add the corresponding amount of potato extract, and place the 4 sets of solutions at 0°C, 10°C, At 30°C and 40°C, after reacting for 35 minutes, measure the absorbance immediately (the measurement wavelength is 475nm is the best). Such as figure 1 .

[0032] (2) The influence of system reaction time on the optimal ...

Embodiment 2

[0058] Weigh 2.0 kg of fresh pineapple peel, wash it twice with tap water, then wash it once with distilled water, then wash it twice with sterile water, then wash it twice with 75% ethanol, irradiate it with ultraviolet light for 5 minutes, and finally cut it into pieces. Crushed, placed in a sterile fermenter, then added 400g of glucose, sealed the fermenter, at 15-25°C, after 25 days of natural fermentation, took out the fermented liquid, put it in a centrifuge tube, centrifuged , get supernatant, carry out decompression concentration, obtain solid sample 10.83g, be pineapple peel ferment.

Embodiment 3

[0060] Weigh 1.0 kg of fresh pineapple peel, wash it twice with tap water, then wash it twice with distilled water, then wash it once with sterile water, then wash it twice with 75% ethanol, irradiate it with ultraviolet light for 5 minutes, and finally cut it into pieces. Crushed, placed in a sterile fermenter, then added 350g of glucose, sealed the fermenter, at 15-25°C, after natural fermentation for 35 days, took out the fermented liquid, put it in a centrifuge tube, centrifuged , get the supernatant, and concentrate under reduced pressure to obtain 6.65g of solid sample, which is pineapple peel enzyme.

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Abstract

The invention relates to pineapple ferment and application of the pineapple ferment as a tyrosinase inhibitor. The pineapple ferment is obtained by naturally fermenting wild yeast on the surfaces of the pineapple peels or pineapple leaves or bromelin in the presence of glucose, and has the characteristics that raw materials are cheap and easy to get and waste can be converted into treasure. By adopting the pineapple ferment and the application of the pineapple ferment, not only can the problems that byproducts of the pineapple processing-pineapple peel resources are wasted and the environment is polluted be solved, but also the pineapple ferment with an effect on inhibiting tyrosinase is obtained by virtue of the natural fermenting technology. The pineapple ferment can be used as a whitening agent in the field of cosmetics and is natural and non-irritating.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a pineapple enzyme, a preparation method thereof and an application for inhibiting tyrosinase. Background technique [0002] Melanin (melanin) is mainly produced by melanocytes in the human body, which can reduce the damage of ultraviolet rays to the skin; however, the abnormal accumulation of melanin will cause hyperpigmentation, and easily cause freckles, age spots, and melasma. The researchers found that tyrosinase plays an important role in the biosynthesis of melanin. In the process of melanin biosynthesis, tyrosinase plays a very important catalytic role. Tyrosinase can promote the conversion of L-tyrosine into L-dopa, and L-dopa is converted into dopaquinone through oxidation, and dopaquinone multipolymerizes to form melanin. [0003] Pineapple pulp belongs to the edible part of pineapple and contains rich nutrients; however, pineapple peel and pineapple leaves are i...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/9789A61Q19/02
CPCA61K8/97A61K8/99A61K2800/85A61Q19/02
Inventor 纪明慧郭飞燕陈光英吕纪行
Owner HAINAN NORMAL UNIV
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