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Preparation method and application of cellulose-SiO2 composite carrier

A composite carrier, cellulose technology, applied in the direction of being fixed on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve the problems of excessive hydrophilicity, easy inactivation, instability, etc. Achieving the effect of decreasing temperature sensitivity, enhancing mechanical strength, and enhancing hydrophobicity

Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since enzymes are generally water-soluble proteins, they often have the following disadvantages: ① unstable and easy to inactivate; ② difficult to recycle and reuse; ③ continuous operation cannot be realized
[0004] However, as a natural polymer material, cellulose still has some shortcomings when used as a carrier for immobilized enzymes, which are mainly reflected in the following two aspects: 1. The hydrophilicity is too strong, and it is easy to absorb other impurities and be polluted when used; 2. The rigidity of organic matter Not strong, durability could be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of cellulose-SiO 2 Preparation method of composite carrier

[0038] (1) Weigh 6 g of microcrystalline cellulose and dissolve it in 100 mL of hydrochloric acid solution with a concentration of 5 mol / L, stir, hydrolyze for 12 hours, centrifuge, and take the precipitate. The precipitated substance is washed and soaked with deionized water, and the precipitate is suspended in deionized water; sodium periodate is added to the solution under stirring until the precipitated substance is evenly dispersed. The reaction was carried out in a dark environment, and the pH value of the solution was kept at 4 during the process, and the temperature was 35°C. After the reaction, add tert-butanol to the solution, and centrifuge to obtain dialdehyde cellulose.

[0039] (2) Add 10mL tetraethylsiloxane to 50mL ethanol-water solution (50% by volume), mix well, then add 0.1mL glacial acetic acid, heat up to 75°C, and after 10 minutes, add N-ammonia dropwise Ethyl-γ-ami...

Embodiment 2

[0042] Embodiment 2 Immobilization test of glucose oxidase.

[0043] Dialdehyde cellulose-SiO obtained in embodiment 1 2 Glucose oxidase was solidified on the composite gel film, and the solidification steps were as follows: prepare a glucose oxidase-phosphate solution (pH=6.5) with a concentration of 1 mg / mL, dialdehyde cellulose-SiO 2 The composite gel film was immersed in the solution, placed in a shaker at 33° C. for oscillating reaction for 4 hours, then the gel film was taken out, washed three times with phosphate buffer solution and deionized water each, to obtain glucose oxidase immobilized on the carrier.

[0044] The immobilized glucose oxidase was subjected to a series of tests, and the results were as follows: the immobilized glucose oxidase immobilized on the carrier can retain the activity of free glucose oxidase up to 72.3%; the enzyme activity recovery rate of the glucose oxidase after repeated use 5 times 78.0%; the enzyme activity of glucose oxidase after 60...

Embodiment 3

[0046] Embodiment 3 A kind of cellulose-SiO 2 Preparation method of composite carrier

[0047] (1) Weigh 4g of pulp and dissolve it in 100mL of hydrochloric acid solution with a concentration of 5mol / L, stir, centrifuge after hydrolysis for 12 hours, and take the precipitate. The precipitated substance is washed and soaked with deionized water, and the precipitate is suspended in deionized water; sodium periodate is added to the solution under stirring until the precipitated substance is evenly dispersed. The reaction was carried out in a light-proof environment, and the pH value of the solution was kept at 3 during the process, and the temperature was 50°C. After the reaction, add tert-butanol to the solution, and centrifuge to obtain dialdehyde cellulose.

[0048] (2) Add 20mL butyl orthosilicate to 60mL ethanol-water solution (50% by volume), mix well, then add 0.5mL glacial acetic acid, heat up to 85°C, and after 15 minutes, add N-aminoethyl ether dropwise yl-γ-aminopro...

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Abstract

The invention provides a preparation method and application of a cellulose-SiO2 composite carrier. The preparation method comprises the following steps: adding an ion solution into dialdehyde cellulose-SiO2 powder; stirring until the powder is dissolved, and centrifuging and de-foaming the solution to prepare a first fixed solution; cooling the first fixed solution to be 20 DEG C to 40 DEG C to prepare a second mixed solution, wherein the cooling time is 60 minutes to 120 minutes; preparing the second mixed solution into a liquid film with the thickness of 0.2mm to 1.0mm or micro-spheres with the diameter of 0.4mm to 1.2mm; putting the liquid film or the micro-spheres into an ethanol-water solution with the volume percent concentration of 15 percent and condensing; washing the condensed and molded thin film or micro-spheres with de-ionized water. The composite carrier provided by the invention has a self-cleaning function; after the composite carrier is used for a solidified enzyme, a lot of enzyme activity of free enzymes can be remained; the enzymes solidified on the composite carrier can be recycled and repeatedly utilized; the temperature sensitivity is greatly reduced when being compared with that of the free enzymes, and the composite carrier has the properties of stability and uneasiness of deactivating.

Description

technical field [0001] The invention relates to the technical field of immobilized enzyme carriers, in particular to a cellulose-SiO immobilized enzyme 2 Preparation method of composite carrier and cellulose-SiO 2 Application of composite carrier in immobilizing enzymes. Background technique [0002] Enzymes belong to biological catalysts. Compared with ordinary chemical reaction catalysts, enzymes can perform highly selective catalytic reactions under relatively mild conditions such as normal temperature and normal pressure. However, because enzymes are generally water-soluble proteins, they often have the following disadvantages: ① unstable and easy to inactivate; ② difficult to recycle and reuse; ③ continuous operation cannot be realized. [0003] Immobilized enzyme technology can improve the above shortcomings. In the immobilized enzyme technology, the most critical thing is the selection or treatment of the carrier material. At present, typical carrier materials inc...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12N11/14
CPCC12N11/14C12N11/12
Inventor 张敏杨俊晖苗庆显陈礼辉黄六莲
Owner FUJIAN AGRI & FORESTRY UNIV
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