Method for preparing high-purity marine polyunsaturated fatty acid phospholipids from cod viscera

A fatty acid phospholipid, high-purity technology, applied in the directions of edible phospholipid composition, chemical instrument and method, application, etc., can solve the problems that the filler is not easy to reuse, affect the quality of phospholipid, and high operating costs, achieve wide and cheap raw material sources, and reduce extraction. Cost, high extraction efficiency

Active Publication Date: 2018-06-01
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many technical defects in these methods: raw materials are mainly extracted from soybeans and egg yolks, thereby robbing humans of food resources; supercritical extraction methods, membrane separation methods and other processes are relatively complicated, and the operation cost is high; inorganic salt precipitation method introduces metal Ions (Zn 2+ 、Cd 2+ etc.), have certain toxicity and affect the quality of phospholipids; silica gel adsorption chromatography often causes dead adsorption, the filler is not easy to reuse, and it is easy to cause environmental pollution, etc.

Method used

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  • Method for preparing high-purity marine polyunsaturated fatty acid phospholipids from cod viscera
  • Method for preparing high-purity marine polyunsaturated fatty acid phospholipids from cod viscera
  • Method for preparing high-purity marine polyunsaturated fatty acid phospholipids from cod viscera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Weigh 100g of frozen cod viscera, crush it with a tissue grinder, add 1000mL of 90% ethanol solution (sodium acetate to make the pH 9.0), stir and extract at room temperature for 2 hours, and filter under reduced pressure to obtain the extract; add the same alkaline The ethanol solution was repeatedly stirred and extracted once, and the extract was obtained by suction filtration. The two extracts were combined, and the pH was adjusted to neutrality with hydrochloric acid; 1:10), stirred for 30 minutes, left to stand and then centrifuged for 10 minutes, the supernatant was concentrated under reduced pressure at 47°C to remove the solvent to obtain crude phospholipids; after the crude phospholipids were dissolved with a small amount of absolute ethanol, the active The white clay is dispersed, the solvent is evaporated, put into the extraction kettle, and the supercritical CO 2 The total phospholipids were obtained by extraction, and the extraction conditions were as follo...

Embodiment 2

[0037] Weigh 100g of frozen cod viscera, grind it with a tissue grinder, add 600mL of 95% ethanol solution (sodium bicarbonate adjusts its pH to 10.0), stir and extract at room temperature for 4 hours, and filter under reduced pressure to obtain the extract; add the same alkaline The ethanol solution was repeatedly stirred and extracted 2 times, each time for 4 hours, and the extract was obtained by suction filtration, the extract was combined, and the pH was adjusted to neutral with hydrochloric acid; 36g of inclusion purifier (beta-cyclodextrin in the purifier) ​​was added to the extract. The weight ratio with activated carbon is 1:10), stirred for 30min, centrifuged for 10min after standing still, took the supernatant and concentrated under reduced pressure at 45°C to remove the solvent to obtain crude phospholipids; after the crude phospholipids were dissolved with a small amount of absolute ethanol, the The activated clay with twice the weight of crude phospholipids is dis...

Embodiment 3

[0039] Weigh 100g of frozen cod viscera, crush it with a tissue grinder, add 1200mL of alkaline ethanol (sodium bicarbonate to adjust its pH to 10.0), stir and extract at room temperature for 4 hours, and filter under reduced pressure to obtain the extract; add the same alkaline The ethanol is repeatedly stirred and extracted once, and the extract is obtained by suction filtration, the extract is combined, and the pH is adjusted to neutrality with hydrochloric acid; 72g of inclusion purifier is added to the extract (the weight ratio of β-cyclodextrin to activated carbon in the purifier is 1 :10), stirred for 40min, centrifuged for 10min, got the supernatant and concentrated under reduced pressure at 43°C to remove the solvent, and obtained the crude phospholipid; The dry solvent is put into the extraction kettle, and the supercritical CO 2 The total phospholipids were obtained by extraction, and the extraction conditions were as follows: the extraction pressure was 22MPa, the ...

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Abstract

The invention provides a method for preparing high-purity marine polyunsaturated fatty acid phospholipid by virtue of codfish viscera. Processes of efficient solvent extraction, rapid purification, ultrasonic-enhanced supercritical extraction, polystyrene gel column chromatography and the like are adopted, so that the method has the advantages of environment friendliness, energy saving and high efficiency. The high-purity marine polyunsaturated fatty acid phospholipid is extracted by virtue of codfish processing wastes. An extraction process is convenient to operate, and extraction efficiency is high; the purity of the phospholipid is more than or equal to 97 percent. According to the method, on one hand, the problem of waste of a large number of residual marine byproduct resources is solved, and environmental pollution is reduced; on the other hand, preparation sources for the phospholipid are enriched, the raw materials are intensive and low-cost, the extraction cost of the phospholipid is reduced, and great ecological, economic and social significance is achieved.

Description

technical field [0001] The invention belongs to the technical field of preparation of marine active substances, and in particular relates to a method for extracting high-purity marine polyunsaturated fatty acid phospholipids from cod viscera. Background technique [0002] Phospholipids are a class of phosphate-containing lipid compounds, which are the basic components of tissue cells, including sphingomyelin and glycerophospholipids. The former is mainly found in the erythrocyte membrane of higher animals, while the latter is abundant in animal liver, brain and ovary. Phospholipids not only have high nutritional value, but also have physiological regulation functions, promote human metabolism, enhance immunity, and prevent diseases. Now, the United States, Europe, Japan and other developed countries have applied phospholipids in clinical practice to prevent brain, heart, liver, tumor and other diseases. In addition, phospholipids also have the characteristics of emulsifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
CPCC07F9/103Y02P20/54
Inventor 韩利文李晓彬何秋霞刘可春张姗姗孙晨韩建王希敏郭敬兰盛文龙
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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