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Method for preparing bone gelatin by ultrasonic assisted enzyme method

A technology of ultrasound and bone gelatin, applied in the field of gelatin preparation, can solve the problems of long enzymatic hydrolysis time, few enzymes, uneven raw materials, etc.

Active Publication Date: 2017-04-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzymatic gel preparation still has problems such as the control of the degree of enzymatic hydrolysis, long enzymatic hydrolysis time, uneven raw materials, and few enzymes.
At present, there is no report on the preparation of gelatin by ultrasonic-assisted enzymatic osteolysis at home and abroad.

Method used

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  • Method for preparing bone gelatin by ultrasonic assisted enzyme method
  • Method for preparing bone gelatin by ultrasonic assisted enzyme method
  • Method for preparing bone gelatin by ultrasonic assisted enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] For specific implementation see figure 1 , the steps include:

[0024] (1) pulverizing the defatted bone particles into defatted bone powder with a particle size of ≤600 μm;

[0025] (2) 500 g of defatted bone powder with a particle size ≤ 600 μm obtained in step (1) was decalcified with 5 L of 0.5 molL hydrochloric acid for 12 hours, washed with water, and then separated into solid and liquid by a centrifuge;

[0026] (3) Add water to the decalcified bone powder obtained after the solid-liquid separation in step (2) and stir to obtain a slurry, then add hydrochloric acid to adjust the pH value of the slurry to 3, and add dry decalcified bone powder to the slurry with a pH value of 3 1‰ protease of the bone meal was subjected to enzymatic hydrolysis for 5 hours, and ultrasonic waves were applied at the same time of enzymatic hydrolysis. The ultrasonic power was 100W, and the ultrasonic time was 20 minutes.

[0027] (4) After step (3) enzymatic hydrolysis, the obtained...

Embodiment 2

[0032] For specific implementation see figure 1 , the steps include:

[0033] (1) pulverizing the defatted bone particles into defatted bone powder with a particle size of ≤600 μm;

[0034] (2) 500 g of defatted bone powder with a particle size ≤ 600 μm obtained in step (1) was decalcified with 5 L of 0.5 molL hydrochloric acid for 12 hours, washed with water, and then separated into solid and liquid by a centrifuge;

[0035] (3) Add water to the decalcified bone powder obtained after the solid-liquid separation in step (2) and stir to obtain a slurry, then add hydrochloric acid to adjust the pH value of the slurry to 3, and add dry decalcified bone powder to the slurry with a pH value of 3 1‰ protease of bone meal was subjected to enzymatic hydrolysis for 5 hours, and ultrasonic waves were applied at the same time of enzymatic hydrolysis. The ultrasonic power was 200W, and the ultrasonic time was 20 minutes.

[0036] (4) After step (3) enzymatic hydrolysis, the obtained slu...

Embodiment 3

[0041] The specific implementation is as follows:

[0042] (1) pulverizing the defatted bone particles into defatted bone powder with a particle size of ≤600 μm;

[0043] (2) 500 g of defatted bone powder with a particle size ≤ 600 μm obtained in step (1) was decalcified with 5 L of 0.5 molL hydrochloric acid for 2 hours, washed with water, and then solid-liquid separation was performed with a centrifuge;

[0044] (3) Add water to the decalcified bone meal obtained after the solid-liquid separation in step (2) and stir to obtain a slurry, then add hydrochloric acid to adjust the pH value of the slurry to 1.5, and add dry decalcified bone powder to the slurry with a pH value of 1.5 The 0.5% protease of the bone meal was subjected to enzymatic hydrolysis for 2 hours, and ultrasonic waves were applied at the same time. The ultrasonic power was 300 W, and the ultrasonic time was 20 minutes.

[0045] (4) After step (3) enzymatic hydrolysis, the obtained slurry is centrifuged to se...

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Abstract

The invention discloses a method for preparing gelatin by an ultrasonic assisted enzyme method, and belongs to the field of gelatin preparation. The method is as follows: using hydrochloric acid for decalcification of defatted bone meal with the particle size of less than or equal to 600 mu m, filtering with gauze, adding water into the obtained decalcified bone meal for mixing to obtain slurry, adding an inorganic acid to adjust the pH value of the slurry to 1.5-4.5, then adding protease for enzymolysis, applying ultrasonic wave to the slurry while the enzymolysis, after solid-liquid separation by a centrifuge, removing the protease from water washing slag, adding water into enzymolyzed ossein for mixing, adjusting the pH value of the slurry, heating for extraction to obtain mixed slurry containing gelatin, using the centrifuge for solid-liquid separation to obtain a gelatin transparent solution, filtering, desalting, performing ion exchange, concentrating and drying to obtain a high quality gelatin product with the gel strength greater than 240Bloomg and viscosity higher than 4.6mPa.s. The yield of ultrasonic assisted enzymatic hydrolysis is improved by over 10% compared with that of conventional enzymatic hydrolysis.

Description

technical field [0001] The invention relates to a method for preparing bone gelatin by an ultrasonic-assisted enzymatic method, and belongs to the technical field of gelatin preparation. Background technique [0002] The main product obtained after mild and irreversible fracture of collagen in connective tissues such as bone, tendon, cartilage, skin, and sarcolemma of animals is called gelatin. Gelatin is an important polymer biomaterial with many excellent physical and chemical properties such as gelling, viscosity, and film-forming properties, and is widely used in food, medicine, and chemical industries. Bone gelatin is gelatin extracted from animal bones. The extraction method has been using the traditional alkaline process. The process route is: pickling, water washing, liming, water washing, neutralization, water washing, extraction, filtration, concentration, drying, etc. process. The liming time of this process route is as long as 2 to 3 months, and the production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/18
CPCC07K14/78C12P21/06
Inventor 赵伟曾小芳杨瑞金
Owner JIANGNAN UNIV
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