Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium

A technology of bacterial cellulose membrane and Phanerochaete protothecoides, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the inconvenience of post-treatment of wastewater, slow growth rate, and reduce the degradation of malachite green. efficiency issues

Inactive Publication Date: 2017-04-26
NANJING UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

However, since white rot fungi exist in fuel wastewater in the form of free mycelium balls, it is not convenient for wastewater post-treatment and ...

Method used

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  • Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium
  • Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium
  • Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium

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preparation example Construction

[0030] Preparation of bacterial cellulose membrane carrier: Acetobacter xylinum NUST4.2 is used as the strain, produced by static fermentation culture, purified and treated to obtain bacterial cellulose wet membrane. The bacterial cellulose membrane was cut into three types of small, medium and large cuboid pieces, the sizes of which were 0.5cm×0.5cm×0.3cm, 1.0cm×0.8cm×0.5cm, 1.5cm×1.2cm× 1.0cm, respectively, sterilized at 121°C for 30 minutes, and set aside.

[0031] Step 3, preparing the spore suspension of Phanerochaete chrysosporium;

[0032] Preparation of spore suspension: Connect Phanerochaete chrysosporium to a solid plate medium, culture at a constant temperature of 30°C for 7 days, then connect the grown spores of Phanerochaete chrysosporium to glass beads and 50mL, 0.85 % of sterilized physiological saline in a Erlenmeyer flask, shake and disperse to obtain a spore suspension of Phanerochaete chrysosporium, and store it in a refrigerator at 4°C for future use.

[...

Embodiment 1

[0046] Step 1 Preparation of solid plate medium: 200g fresh potato pieces, add water and boil for 30min, filter through 8 layers of gauze, take the filtrate, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.75g, agar 20g, dilute to 1L, pH natural.

[0047] Step 2: Preparation of the bacterial cellulose membrane carrier: Acetobacter xylinum NUST4.2 is used as the strain, produced by static fermentation, purified and treated to obtain the bacterial cellulose wet membrane. Cut the obtained bacterial cellulose membrane into 5 large cuboid pieces (1.5cm×1.2cm×1.0cm), sterilize at 121°C for 30min, and set aside.

[0048] Step 3 Preparation of spore suspension: Connect Phanerochaete chrysosporium to its solid plate culture medium, culture at a constant temperature of 30°C for 7 days, then connect the spores of the grown fungus to glass beads and 50mL, 0.85% Sterilized physiological saline is shaken and dispersed to obtain a certain concentration of Phanerochaete...

Embodiment 2

[0053] Step 1 Preparation of solid plate medium: 200g fresh potato pieces, add water and boil for 30min, filter through 8 layers of gauze, take the filtrate, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.75g, agar 20g, dilute to 1L, pH natural.

[0054] Step 2: Preparation of the bacterial cellulose membrane carrier: Acetobacter xylinum NUST4.2 is used as the strain, produced by static fermentation, purified and treated to obtain the bacterial cellulose wet membrane. Cut the obtained bacterial cellulose membrane into 10 large (1.5cm×1.2cm×1.0cm) cuboid pieces, sterilize at 121° C. for 30 minutes, and set aside.

[0055] Step 3 Preparation of spore suspension: Connect Phanerochaete chrysosporium to its solid plate culture medium, culture at a constant temperature of 30°C for 7 days, then connect the spores of the grown fungus to glass beads and 50mL, 0.85% Sterilized physiological saline is shaken and dispersed to obtain a certain concentration of Phane...

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Abstract

The invention discloses a method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium. The method comprises the following steps: preparing a solid plate culture medium and obtaining activated acetobacter xylinum NUST4.2 as a strain; performing static fermentation culture to obtain bacterial cellulose membrane carriers and cutting to obtain small cuboids with different sizes; preparing a spore suspension of phanerochaete chrysosporium; preparing a liquid culture medium; adding the bacterial cellulose membrane carriers with different sizes and numbers into the liquid culture medium and putting the spore suspension of the phanerochaete chrysosporium into the liquid culture medium to prepare the immobilized phanerochaete chrysosporium; and performing de-coloring test on malachite green dye wastewater by adopting the obtained immobilized phanerochaete chrysosporium to determine the influence of the bacterial cellulose membrane carriers on the degradation of the malachite green dye wastewater by the immobilized phanerochaete chrysosporium. According to the method disclosed by the invention, the efficiency and stability of the phanerochaete chrysosporium in degrading the malachite green are improved.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment, in particular to a method for degrading malachite green by immobilizing Phanerochaete chrysosporium on a bacterial cellulose film. Background technique [0002] As a synthetic organic compound, malachite green is widely used in the dyeing of silk, wool and leather in the textile and leather industries. However, because it can inhibit the growth of most protozoa or fungi, it is difficult to be degraded by most microorganisms. At the same time, malachite green has high toxicity, teratogenicity, and mutagenicity, and a large amount of it remains in water bodies and The soil not only causes serious environmental pollution, but also poses a serious threat to human life and health. The traditional method of treating dye wastewater requires a lot of catalysts, chemical reagents and energy consumption, which not only increases the treatment cost but also causes secondary pollution to the e...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12P19/04C02F3/34C12R1/645C12R1/02C02F101/38
CPCC12N11/12C02F3/347C02F2101/308C02F2101/38C12P19/04
Inventor 孙东平梁光芸朱春林自强陈春涛黄洋顾焱
Owner NANJING UNIV OF SCI & TECH
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