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Chemically modified electrode for quantitative determination of folic acid and preparation method of electrochemical sensor

A quantitative detection and chemical modification technology, applied in the field of electrochemical sensing, can solve the problems of being susceptible to interferences, high detection limits, and complicated pre-processing of samples, and achieve enhanced electrocatalytic activity, detection performance, and good stability. , the effect of low cost

Active Publication Date: 2017-04-19
湖南中检博科检验检疫服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by this invention is to provide a chemically modified electrode for quantitative detection of folic acid and Preparation method of electrochemical sensor

Method used

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  • Chemically modified electrode for quantitative determination of folic acid and preparation method of electrochemical sensor
  • Chemically modified electrode for quantitative determination of folic acid and preparation method of electrochemical sensor
  • Chemically modified electrode for quantitative determination of folic acid and preparation method of electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Preparation of a chemical electrode for quantitative detection of folic acid

[0038] S1. Take 40 mg of multi-walled carbon nanotubes, add 1 mL of concentrated nitric acid and 3 mL of concentrated sulfuric acid, and then sonicate for 4 hours to obtain a suspension;

[0039] S2. Add ethanol to the above-mentioned suspension to 10 mL, and centrifuge; then add water and centrifuge until the pH value of the filtrate is neutral, and then dry the precipitate to obtain carboxylated multi-walled carbon nanotubes (MWCNTs);

[0040] S3. Take 4.5 mg of carboxylated multi-walled carbon nanotubes, add 3 mL of water for ultrasonic dispersion, and then add 0.19 g of glucose to form solution A;

[0041] S4. Take 0.026g of silver nitrate in 3mL of water and stir to dissolve, then add 0.5mL of NH 3 ·H 2 O forms solution B;

[0042] S5. Add solution B to solution A and stir for 0.5h. After aging for 2h, after centrifugation and washing, dry in an oven at 60°C for 12h to ob...

Embodiment 2

[0045] Embodiment 2 A kind of electrochemical sensor for quantitative detection of folic acid

[0046] The Ag / MWCNTs composite film modified electrode prepared in Example 1 was used as a working electrode, a saturated calomel electrode was used as a reference electrode, and a platinum wire electrode was used as an auxiliary electrode to assemble a three-electrode test system, and connected to an electrochemical workstation for folic acid Electrochemical sensors for quantitative detection.

Embodiment 3

[0047] Example 3 The electrical performance test of the electrochemical sensor used for the quantitative detection of folic acid

[0048] (1) Comparison of electron transfer performance of different electrodes

[0049] In the three-electrode test system prepared as in Example 2, the MWCNTs modified electrode (a) and the Ag / MWCNTs composite film modified electrode (b) prepared in Example 1 were used as working electrodes at 1.0mmol / L K 3 [Fe(CN) 6 ] The AC impedance and cyclic voltammetry tests were carried out in the bottom liquid mixed with 0.1mmol / L KCl, and the AC impedance test conditions were: frequency range 10 5 ~0.1HZ, amplitude 5mV, potential 0.18V; cyclic voltammetry test conditions are: potential range -0.4~1.0V, scanning speed 0.1V / s, the test results are as follows figure 2 . from figure 2 Visible electrochemical impedance is respectively 60 Ω and 30 Ω on two kinds of working electrodes of above-mentioned MWCNTs and Ag / MWCNTs composite film modification elec...

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Abstract

The invention discloses a chemically modified electrode for quantitative determination of folic acid and a preparation method of an electrochemical sensor. The method comprises the following steps: S11, fetching multiwalled carbon nanotube, adding concentrated nitric acid and concentrated sulfuric acid and then carrying out ultrasonic treatment so as to obtain a suspension; S 12, adding ethanol into the above suspension, and carrying out centrifugal separation; adding water and carrying out centrifugal separation until pH value of a filtrate is 6-8, and drying sediment to obtain carboxylated multiwalled carbon nanotube; S13, fetching the carboxylated multiwalled carbon nanotube, adding water and carrying out uniform ultrasonic dispersion, and adding glucose to form a solution A; S14, adding silver nitrate into water and stirring and dissolving, dropwise adding concentrated NH3.H2O to form a solution B; S15, adding the solution B into the solution A, stirring, ageing, centrifuging and washing, and drying by a drying oven to obtain an Ag / MWCNTs composite material; and S16, dispersing the Ag / MWCNTs composite material in an organic solvent to obtain a dispersion liquid; and coating and fixing the dispersion liquid on the surface of a cleaned glassy carbon electrode so as to obtain the electrode. The chemically modified electrode prepared by the above method has low detection limit and good selectivity.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensing, in particular to a chemically modified electrode for quantitative detection of folic acid and a preparation method of an electrochemical sensor. Background technique [0002] Folic acid has important physiological functions in the human body. It cannot be directly synthesized in the human body, but must be obtained through diet. In the human body, folic acid can be converted into biologically active tetrahydrofolate after being absorbed. It participates in the synthesis and manufacture of ribonucleic acid and deoxyribonucleic acid, and plays a very important role in the synthesis and transformation of purine nucleic acid and pyrimidine nucleotide in cells. important role. Folic acid is closely related to the synthesis of DNA. If a pregnant woman is severely deficient in folic acid, it will hinder the synthesis of DNA in the fetus, and the cell division will be weakened, and the k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 李俊华许志锋刘梦琴张复兴刘兴欧亚平
Owner 湖南中检博科检验检疫服务有限公司
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