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Adipose-derived mesenchymal stem cell transportation protective liquid

A technology for stem cells and protective solution, which is applied in the field of adipose-derived mesenchymal stem cells transporting protective solution, can solve the problems such as the inability to effectively improve the patient's immune system status, the inability to guarantee cell stability, and the insignificant treatment effect, and to achieve easy storage and long-distance The effect of distance transportation, prevention of repeated attacks, and safe use

Inactive Publication Date: 2017-04-05
上海佳祥健康管理咨询有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in actual treatment, the cell treatment process before infusion is relatively simple, and the stability of cells during use cannot be guaranteed, and cell activity cannot be maintained for a long time, so it is not suitable for long-distance transportation. In clusters, after systemic injection of adipose-derived mesenchymal stem cells, most of the stem cells cannot reach the lesion, making the therapeutic effect insignificant
However, local injection cannot effectively improve the patient's immune system and fundamentally alleviate the course of the disease.

Method used

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  • Adipose-derived mesenchymal stem cell transportation protective liquid
  • Adipose-derived mesenchymal stem cell transportation protective liquid
  • Adipose-derived mesenchymal stem cell transportation protective liquid

Examples

Experimental program
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specific Embodiment 1

[0030] Specific example 1: The adipose-derived mesenchymal stem cell transportation protection solution is composed of 50% human serum albumin stock solution, 9.5% glucose solution for injection, 40% physiological saline for injection and 0.5% heparin sodium.

specific Embodiment 2

[0031] Specific example 2: The adipose-derived mesenchymal stem cell transportation protection solution is composed of 50% human serum albumin stock solution, 29.5% glucose solution for injection, 20% physiological saline for injection and 0.5% heparin sodium.

specific Embodiment 3

[0032] Specific Example 3: The adipose-derived mesenchymal stem cell transport protection solution is composed of 30% human serum albumin stock solution, 30% glucose solution for injection, 39.5% physiological saline for injection and 0.5% heparin sodium.

[0033] The stock solution of human serum albumin is the stock solution of human serum albumin produced by Harbin Shiheng Bioengineering Pharmaceutical Co., Ltd. with the approval number of S10920005, and the concentration of the stock solution is 10%.

[0034] The adipose-derived mesenchymal stem cells are resuspended and added to the adipose-derived mesenchymal stem cell transport protection solution, including the following steps:

[0035] ① Use a micro-sampling gun to draw a small amount of cell suspension for cell counting and cell viability detection, discard dead cells, and count live cells; if the concentration of live cells is 6×10 5 -7×10 5 cells / 1ml, go to step ③; otherwise, continue to adjust with step ②;

[00...

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Abstract

The invention discloses adipose-derived mesenchymal stem cell transportation protective liquid. Adipose-derived mesenchymal stem cells are adjusted in a resuspension manner and added into the adipose-derived mesenchymal stem cell transportation protective liquid, and the concentration of the adipose-derived mesenchymal stem cells in the transportation protective liquid ranges from 6*10<5> cells / ml to 7*10<5> cells / ml. The adipose-derived mesenchymal stem cell transportation protective liquid has the beneficial effects of being high in stability, convenient to preserve and transport and capable of guaranteeing the vitality of the cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a transport protection solution for adipose-derived mesenchymal stem cells suitable for long-distance transport. Background technique [0002] The incidence of inflammatory bowel disease (IBD) in Western countries is higher than that in Asian countries, but in recent years, the incidence in my country has gradually increased. According to the survey results of the Digestive Medicine Society of Chinese Medical Association in 2007, the number of IBD cases in my country in the past 10 years The prevalence rate of ulcerative colitis (UC) was 11.6 / 1 million, and that of Crohn's disease (CD) was about 1.4 / 1 million. Crohn's disease is a chronic inflammatory disease. The lesions mainly involve the intestines, and fistula may form in the most severe cases. The exact etiology of the disease is still unclear. The currently generally accepted pathogenesis of CD is that environmental and infectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
Inventor 路建伟敖星星
Owner 上海佳祥健康管理咨询有限公司
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