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Preparation method of low-molecule soybean polypeptide with healthcare function

A soybean polypeptide and low molecular technology, which is applied in the field of preparation of low molecular soybean polypeptides, can solve the problems of intestinal damage of livestock and poultry, waste of raw materials, environmental pollution, etc., so as to increase the number of viable bacteria, increase the added value, and increase the content Effect

Inactive Publication Date: 2017-03-29
ZHONGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because soybean meal has a large molecular weight and contains a large amount of anti-nutritional factors such as non-starch polysaccharides, soybean antigenic protein and residual protease inhibitors, soybean meal needs to go through a complex digestion process in the gastrointestinal tract before it can be absorbed and utilized, and it will also affect the intestinal tract of livestock and poultry. Injury to the intestinal tract, especially the intestinal tract of young livestock and poultry
Studies have shown that the nutritional diarrhea and low immunity of young animals are closely related to the characteristics of soybean meal, which directly cause problems such as low protein utilization, waste of raw materials, environmental pollution and increased breeding costs.

Method used

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  • Preparation method of low-molecule soybean polypeptide with healthcare function
  • Preparation method of low-molecule soybean polypeptide with healthcare function
  • Preparation method of low-molecule soybean polypeptide with healthcare function

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preparation example Construction

[0034] The preparation method of the Clostridium butyricum bacterial liquid used in the present invention is: take -20 ℃ and preserve the Clostridium butyricum bacterial classification 200 μ l in the glycerin tube and inoculate in the test tube that the proliferation medium (RCM) of 9ml is housed, then culture The base is covered with about 2cm of liquid paraffin, and cultured at 37°C for 48 hours to form buds. Put the above-mentioned spore culture in a water bath at 80°C for 10 minutes, then transfer 1ml of the inoculum to sterilized test tubes containing 9ml of proliferation medium, and cultivate for 16-20h until the logarithmic phase can be used for inoculation to in the fermentation medium.

[0035] Seed medium (clostridium proliferation medium, RCM medium for short): 3g of yeast extract, 10g of beef extract, 10g of trypsin, 5g of glucose, 1g of soluble starch, 5g of sodium chloride, 3g of sodium acetate trihydrate, half Cystine hydrochloride 0.5g, 0.5% methylene blue 0.2...

Embodiment 1

[0037] A preparation method of low-molecular-weight soybean polypeptide with health care function, comprising the following steps:

[0038] (1) Soybean meal crushing

[0039] Using soybean meal as a raw material, the soybean meal is ultrafinely pulverized by an ultrafine pulverizer, and passed through a 40-mesh sieve to obtain soybean meal micropowder;

[0040] (2) One time non-starch polysaccharide enzymatic hydrolysis soybean meal

[0041] Using soybean meal powder as substrate, cellulase 200U / g substrate, pectinase 500U / g substrate, endo-β-glucanase 500U / g substrate and phytase 200U / g substrate, at 50 °C, pH 4.5, react for 7 hours to obtain an enzymatic hydrolysis soybean meal; dry at 90 °C to inactivate the enzyme;

[0042] (3) Secondary protease hydrolysis of soybean meal

[0043] Using the primary enzymolysis soybean meal in step (2) as a substrate, add 800 U / g of neutral protease as a substrate, and react for 5 hours at 50°C and pH 7.0 to obtain a second enzymolysis ...

Embodiment 2

[0049] A preparation method of low-molecular-weight soybean polypeptide with health care function, comprising the following steps:

[0050] (1) Soybean meal crushing

[0051] Using soybean meal as a raw material, the soybean meal is ultrafinely pulverized by an ultrafine pulverizer, and passed through a 60-mesh sieve to obtain soybean meal micropowder;

[0052] (2) One time non-starch polysaccharide enzymatic hydrolysis soybean meal

[0053] Using soybean meal powder as substrate, add 250U / g cellulase substrate, 450U / g pectinase substrate, 600U / g β-glucanase substrate and 300U / g phytase substrate, at 45℃ , under the condition of pH 5, react for 7 hours to obtain an enzymatically hydrolyzed soybean meal; dry at 85°C to inactivate the enzyme;

[0054] (3) Secondary protease hydrolysis of soybean meal

[0055] Using the primary enzymolysis soybean meal in step (2) as a substrate, add neutral protease 1000U / g substrate, and react for 6 hours under the conditions of 45°C and pH6.5...

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Abstract

The invention discloses a preparation method of a low-molecule soybean polypeptide with a healthcare function. The preparation method comprises the following steps of: carrying out superfine grinding on soybean pulp, carrying out enzymolysis for the first time by using a complex enzyme of cellulase, pectinase, endo-beta- glucanase and phytase, and adding neutral protease to carry out enzymolysis for the second time; and taking the soybean pump after the twice enzymolysis as a matrix, and carrying out solid fermentation by using clostridium butyricum, so as to obtain the low-molecule soybean polypeptide. According to the preparation method disclosed by the invention, an enzymolysis and biological fermentation combined manner is adopted to carry out biological conversion on the soybean pulp; through twice enzymolysis and once fermentation, the soybean pump protein is firstly hydrolyzed into a low-molecule peptide chain, and then the low-molecule polypeptide product with a clostridium butyricum living bacteria agent is prepared by taking the low-molecule peptide chain as a matrix, so that the content of main non-starch polysaccharides and the protein molecular weight in the soybean pulp are reduced and the content of the living bacteria agent in the soybean pulp is improved. The preparation method disclosed by the invention is simple, wide in raw material source, low in production cost and easy to industrially popularize, and has favorable social and economic benefits.

Description

technical field [0001] The invention relates to a preparation method of low-molecular soybean polypeptide with health care function, belonging to the technical field of soybean meal deep processing. Background technique [0002] Soybean meal is the main protein source of livestock and poultry diet. It is rich in protein, essential amino acids and other nutrients, and is widely used in the feed industry. However, because soybean meal has a large molecular weight and contains a large amount of anti-nutritional factors such as non-starch polysaccharides, soybean antigenic protein and residual protease inhibitors, soybean meal needs to go through a complex digestion process in the gastrointestinal tract before it can be absorbed and utilized, and it will also affect the intestinal tract of livestock and poultry. The intestinal tract, especially the intestinal tract of young livestock and poultry, can cause damage. Studies have shown that nutritional diarrhea and low immunity of...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K50/60A23K10/37A23K10/12A23K10/14A23K20/147
CPCY02P60/87
Inventor 职爱民张小宇高松李建新聂卉闵玉涛宋彦显郭林杨联芝李靖靖
Owner ZHONGZHOU UNIV
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