Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mercury ion fluorescence detection nano probe preparation and application thereof

A technology for fluorescence detection and nanoprobes, applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc.

Active Publication Date: 2017-03-22
厦门信德科创生物科技有限公司
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of nanotechnology, the traditional single-functional nanomaterials are increasingly unable to meet the demand. Therefore, it is necessary to develop a multifunctional probe that can simultaneously realize the detection of Hg in various types of samples. 2+ The detection and rapid removal functions to meet the higher requirements of the environment, food mercury pollution detection and human mercury poisoning detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mercury ion fluorescence detection nano probe preparation and application thereof
  • Mercury ion fluorescence detection nano probe preparation and application thereof
  • Mercury ion fluorescence detection nano probe preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as figure 1 As shown, the present invention discloses the preparation and application of a mercury ion fluorescence detection nanoprobe, the specific steps are as follows:

[0024] Au-Fe 3 o 4 Synthesis of composite nano-concave spheres: take a 100mL beaker, add weighed 1.8mmol FeCl in turn 3 Solid and 40mL ultrapure water, magnetically stirred, after the solution is clarified, add 4.8mmol trisodium citrate dihydrate, and continue to stir. At this time, the solution will change from orange yellow to green, indicating that Fe 3+ partly reduced to Fe 2+ . After the trisodium citrate dihydrate is completely dissolved, add 7.2mmol urea and 0.36g polyacrylamide solid powder in sequence, and then add 3.6mL 0.1M HAuCl dropwise after obtaining a clear solution 4 Aqueous solution, then continue to stir at room temperature for 1 hour, the solution will gradually turn purple-black. At this time, the obtained solution was transferred into the polytetrafluoroethylene lini...

Embodiment 2

[0030] Experimental purpose and method: In order to characterize the microstructure of the synthesized nanoprobes, this example takes the nanoprobes synthesized in Example 1 as the research object, and through scanning electron microscopy, projection electron microscopy, powder X-ray diffraction and VSM magnetic performance tests, For specific experimental methods, please refer to the corresponding standard operations and will not repeat them here.

[0031] Experimental results:

[0032] Such as figure 2 (a), Au-Fe 3 o 4 The composite nano-concave spheres showed obvious concave spherical shape under the scanning electron microscope. figure 2 As can be seen in (b), the Au-Fe 3 o 4 The shape and size of the composite nano-concave spheres are uniform, showing an obvious concave spherical shape, and the average particle diameter is about 163nm.

[0033] In order to further confirm the structure of the composite nano concave sphere, its crystal structure was characterized b...

Embodiment 3

[0036] Experimental purpose and method: In order to determine the detection range and detection line of the synthetic nanoprobe, this embodiment takes the synthetic nanoprobe in Example 1 as the experimental object, and in 0.5mL 1mg / mL DNA functionalized Au-Fe 3 o 4 Add 0.1mL of different concentrations of Hg 2+ After the solution was shaken evenly, its fluorescence emission spectrum was detected.

[0037] Experimental results:

[0038] Such as image 3 As shown in (a), with Hg 2+ As the concentration increased from 0 to 50nM, the fluorescence intensity of the solution decreased gradually. image 3 (b) shows the Au-Fe 3 o 4 The Fluorescence Intensity and Hg of Composite Concave Nanosphere-CdTe Quantum Dots System at 536nm 2+ The relationship between concentration, in the range of 0-50nM, there is no linear correlation between the two, and when Hg 2+ When the concentration is in the range of 0-10nM, there is a good linear relationship between the two, and the linear reg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of a nano probe design application, and discloses a mercury ion fluorescence detection nano probe preparation and an application thereof. By an optimization design of the nanoparticles, by adjusting the mol ratio of an Au and Fe3O4 precursor, the size of the synthesized nano probe can be regulated and controlled, an appropriate base sequence is used for Hg<2+>, a T-Hg<2+>-T structure is established; through reasonable design of the nano probe, the nano probe has single detection capability, the detection lower limit is 0.46 nM, which is far lower than the highest allowed concentration of 30 nM of Hg<2+> in drinking water prescribed by world health organization, the used nano probe passes through a cysteine solution, and the T-Hg<2+>-T structure is dissociated so that the nano probe is regenerated and reused. The probe can be used for detecting Hg<2+> in the samples such as environment, food and human body fluid.

Description

technical field [0001] The invention relates to the design and application field of nanoprobes, in particular to the preparation and application of a mercury ion fluorescence detection nanoprobe. Background technique [0002] Mercury ions (Hg 2+ ) is one of the most toxic heavy metal ions, and exists in a large amount in various biological wastes and pollutants. Hg in the environment 2+ It is easily converted into methylmercury by microorganisms. After entering the body through the food chain, due to its persistence, easy migration and bioaccumulation, it will cause serious damage to organisms. Therefore, Hg in water resources 2+ The detection and management of the problem has become an imminent problem. In recent years, researchers have developed many methods for Hg 2+ Nanoprobes for detection, mainly based on Hg 2+ Enhanced peroxidase-like activity and Hg of noble metal nanoparticles 2+ It can specifically combine with thymine (T) to form "T-Hg 2 + -T" stable struct...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64B82Y30/00B82Y40/00
Inventor 林得志王全胜陈滨晖
Owner 厦门信德科创生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com