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Method for improving activity of anaerobic ammonium oxidation embedded particles

A technology of anaerobic ammonium oxidation and activity, applied in the field of wastewater treatment, can solve problems such as difficulty in increasing the density and activity of anammox bacteria, unsatisfactory treatment efficiency of nitrogen and other pollutants, and limited stability of embedded bacteria, etc., to achieve The effect of increasing the number, increasing the amount of microorganisms, and facilitating operation

Active Publication Date: 2017-03-22
BEIJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Based on the problems existing in the prior art, the present invention provides a method and application capable of improving the activity and denitrification performance of the anammox-embedded bacteria, which solves the difficulty in increasing the density of the anammox bacteria in a fixed area in the prior art And the low activity leads to the limited stability of the embedded bacteria in the process of wastewater denitrification treatment, and the treatment efficiency of nitrogen and other pollutants in wastewater is not ideal.

Method used

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  • Method for improving activity of anaerobic ammonium oxidation embedded particles
  • Method for improving activity of anaerobic ammonium oxidation embedded particles
  • Method for improving activity of anaerobic ammonium oxidation embedded particles

Examples

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Effect test

Embodiment 1

[0036] Example 1: Effects of Different Signaling Molecules on the Activity of Anammox Embedding Beads

[0037] Nutrient solution and different signal molecules C4-HSL, C6-HSL, and C8-HSL were added to the anaerobic ammonium oxidation seed sludge, and the concentrations of the three signal molecules were set to 0.05 μM, 1.5 μM and 3.0 μM, respectively. (The three signal molecules were first dissolved in dimethyl sulfoxide, and the concentration of the prepared three signal molecule solutions was 20mg / mL), and the nutrient solution and the signal molecule solution were used for intermittent culture for 30 days (the concentration of ammonia nitrogen in the effluent was the concentration of ammonia nitrogen in the influent 20% of the time is one cycle), and then the anammox seed sludge is washed 3 times with a potassium bicarbonate buffer solution with a pH of about 7.0 and then centrifuged. A mixed solution of 10 wt% polyvinyl alcohol (PVA) and 2 wt% sodium alginate (SA) was prep...

Embodiment 2

[0040] Example 2: The effect of the volume ratio of embedding agent and anammox seed sludge on the activity of anaerobic ammonium oxidation embedded pellets after adding 1.5 μM C4-HSL

[0041] Experiments were carried out on the influence of signal molecules on the activity of anammox embedding pellets when the volume ratios of embedding agent and anammox seed mud were 1:0.5, 1:1, and 1:2, and the experimental operation steps were the same as the implementation example 1.

[0042] Test and analysis method:

[0043] Nessler's reagent photometry was used for the determination of ammonia nitrogen, UV spectrophotometry was used for the determination of nitrate nitrogen, and N-(1-naphthyl)-ethylenediamine photometric method was used for the determination of nitrite nitrogen (both using UV spectrophotometer DR5000, Hach Corporation, USA) ), DO was measured by HQ30d dissolved oxygen meter (Hach Company, USA), and pH was measured by pHS-3c pH meter (Shanghai Precision Scientific Inst...

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Abstract

The invention discloses a method for improving activity of anaerobic ammonium oxidation embedded particles and belongs to the field of wastewater treatment. Quorum sensing signal molecules of AHLs are added to seed sludge when anaerobic ammonia oxidation seed sludge is cultured and acclimatized, the cultured sludge is mixed with a PVA-SA (polyvinyl acetate-sodium alginate) embedding agent, signal molecules are added again in the mixing process, and the PVA-SA anaerobic ammonium oxidation embedded particles are prepared after the mixture is crosslinked for several hours. With the adoption of the method, the activity is substantially improved, the preparation process is simple, the operation is convenient and the performance is stable.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and relates to a microorganism immobilization technology commonly used in the wastewater treatment process, in particular to a method capable of improving the activity of anaerobic ammonium oxidation embedded particles. Background technique [0002] The denitrification process based on anaerobic ammonium oxidation has a good application prospect in the wastewater biological denitrification process due to its high denitrification efficiency, low energy consumption and low cost. In this process, anammox bacteria use nitrite as electron acceptor to oxidize ammonia nitrogen into nitrogen gas. Compared with the traditional denitrification process, the anaerobic ammonia oxidation system has the advantages of less oxygen demand, no need to add organic carbon source, and less residual sludge generated. Related studies have shown that the growth rate of anammox bacteria is extremely slow, and the doub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/30C12N11/14C12N11/08
CPCC02F3/302C02F3/307C02F3/34C02F3/342C02F2203/004C12N11/08C12N11/14
Inventor 李军张晶曾金平卞伟张彦灼梁东博
Owner BEIJING UNIV OF TECH
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