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Application of carbon monoxide releasing molecules in preparation of anti-bovine viral diarrhea virus drug

A technology for bovine viral diarrhea and carbon monoxide, which is applied in the field of antiviral research to achieve significant antiviral ability, inhibition of viral infection, and mature production technology.

Inactive Publication Date: 2017-03-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly used for research and development, and has not been reported as a drug or other uses

Method used

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  • Application of carbon monoxide releasing molecules in preparation of anti-bovine viral diarrhea virus drug
  • Application of carbon monoxide releasing molecules in preparation of anti-bovine viral diarrhea virus drug
  • Application of carbon monoxide releasing molecules in preparation of anti-bovine viral diarrhea virus drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Effect of CORM-2 on MDBK cells infected by BVDV

[0024] Step 1, CORM-2 treatment of BVDV-infected MDBK cells

[0025] S1. Effects of different concentrations of CORM-2 on BVDV replication

[0026] MDBK cells in the logarithmic growth phase were diluted with DMEM medium containing 10% fetal bovine serum and seeded in 6-well plates, 2 mL per well. When cultured to 70%-80% confluence, inoculate the BVDV strain diluted with DMEM, and absorb for 1 hour; discard the virus solution, add 1mL PBS to wash once, and add different strains prepared with DMEM maintenance solution prepared with 3% fetal bovine serum. Concentrations (50 μM, 100 μM and 150 μM) of CORM-2; at the same time, inactivated CORM-2 (150 μM) was prepared with 3% fetal bovine serum in DMEM maintenance solution as a control, 2 mL per well, and cultured at 37°C and 5% CO2 The box continued to cultivate, and the cells and supernatant were collected at 24hpi.

[0027] S2. The effect of CORM-2 on the rep...

Embodiment 2

[0047] Example 2 Effect of CORM-2 on MDBK cell proliferation activity

[0048] MDBK cells in the logarithmic growth phase were diluted with DMEM medium containing 10% fetal bovine serum and inoculated in a 96-well plate at 100 μL / well. To prevent evaporation, the peripheral wells of the 96-well cell culture plate were filled with the same volume of sterile PBS. Placed at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours, and add the test drug CORM-2. Set treatment groups with different concentrations of CORM-2 (CORM-2 was diluted to 9 different concentrations of 0 μM, 25 μM, 50 μM, 100 μM, 150 μM, 200 μM, 300 μM, 400 μM, and 500 μM). Refer to the CCK-8 kit instructions for MDBK cell activity detection. Use a 100 μL pipette to discard the old medium one by one, add PBS to wash once, then add the CORM-2 solution with a concentration gradient prepared in advance, 100 μL per well, and each concentration gradient experiment well has 6 replicate wells, and the control Wells a...

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Abstract

The invention relates to application of carbon monoxide releasing molecules in preparation of an anti-bovine viral diarrhea virus drug, and belongs to the technical field of antiviral researches. The drug contains carbon monoxide releasing molecules and one or more pharmaceutically acceptable adjuvants; the effective concentration of the carbon monoxide releasing molecules for resisting bovine viral diarrhea virus is 50 to 300 [mu] mol / L. According to the application, methods of qPCR, TCID50 and Western Blot are first adopted to detect the influence of the carbon monoxide releasing molecules on the replication of the bovine viral diarrhea virus in MDBK cells so as to further clarify the influence of the carbon monoxide releasing molecules on virus adsorption and internalizationand to detect the influence of treatment of the carbon monoxide releasing molecules with different concentrations on the proliferative activity of the MDBK cells. The role of the carbon monoxide releasing molecules in resistance to the bovine viral diarrhea virus, and a basis is provided for the preparation of the anti-bovine viral diarrhea virus drugs.

Description

technical field [0001] The invention belongs to the technical field of antiviral research, and in particular relates to the application of carbon monoxide releasing molecules in the preparation of anti-bovine viral diarrhea virus drugs. Background technique [0002] Bovine viral diarrhea virus (Bovine viral diarrhea virus, BVDV) is a representative species of Pestivirus in the Flaviviridae family. It mainly infects cattle and causes diseases. The main features are sexual infection and immunosuppression, and it is an important viral disease of cattle that has caused great harm to the global cattle industry. [0003] At present, the prevention and control of BVDV mainly adopts vaccines and antibiotics. Now the main BVDV vaccines include conventional vaccines and new vaccines. Conventional vaccines mainly use cell culture to obtain BVDV strains, and then obtain attenuated or inactivated vaccines of these strains. Animals infected with antigenic BVDV strains were not effectiv...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K9/19A61K9/14A61P1/12A61P31/14
CPCA61K33/24A61K9/0019A61K9/14A61K9/19
Inventor 肖书奇周恩民李爽蒲锋星
Owner NORTHWEST A & F UNIV
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