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Dendrobium candidum tissue culture seedling culture medium for increasing alkaloid and preparing method thereof

A technology for Dendrobium officinale and tissue culture seedlings is applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., to achieve the effects of improving reliability and industrialized production efficiency, shortening disinfection time, and reducing production costs.

Inactive Publication Date: 2017-03-22
许焕明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides an alkaloid-increased culture medium of Dendrobium candidum tissue culture seedlings and a preparation method thereof, in order to solve the problems of how to increase the content of alkaloids, polysaccharides and amino acids in the main active components of Dendrobium candidum in the existing tissue culture medium, and improve economic benefits, etc.

Method used

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  • Dendrobium candidum tissue culture seedling culture medium for increasing alkaloid and preparing method thereof

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preparation example Construction

[0071] The preparation method of the Dendrobium candidum tissue culture medium for improving alkaloids comprises the following steps:

[0072] S1: crush the apples with a grinder, add 800-1500 parts of water, and pass the obtained juice through a 3000-5000 mesh sieve to obtain apple juice;

[0073] S2: Apple juice, citric acid, sodium o-benzoylsulfonimide, tea polyphenols, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt prepared in step S1 Salt, zinc salt, manganese salt, copper salt, iron salt, vitamin, amino acid, indole butyric acid, gibberellin, 6-benzyladenophyllin, naphthalene acetic acid, kinetin, asparticine, solubilizer, dispersion Mixing agent, compatibilizer, 2000-2500 parts of water, stirring for 25-53min at microwave power of 132-175W, temperature of 55-60°C, and rotation speed of 200-400r / min, to obtain mixed solution A;

[0074] S3: Add conditioner, stabilizer and antibacterial agent to the ...

Embodiment 1

[0077] A culture medium for Dendrobium officinale seedlings with improved alkaloids, in parts by weight, comprising the following raw materials: 280 parts of apple juice, 3 parts of citric acid, 0.04 parts of sodium o-benzoylsulfonylimide, and 2.2 parts of tea polyphenols 3.5 parts of potassium salt, 2.6 parts of phosphorus salt, 2.2 parts of nitrogen salt, 1 part of calcium salt, 1 part of magnesium salt, 0.5 part of iodine salt, 0.3 part of boron salt, 0.25 part of molybdenum salt, 0.35 part of zinc salt, 0.2 part of manganese salt 0.15 parts of copper salts, 0.16 parts of iron salts, 0.2 parts of vitamins, 0.16 parts of amino acids, 0.1 parts of indole butyric acid, 0.1 parts of gibberellin, 0.08 parts of 6-benzyladenophyllin, 0.12 parts of naphthaleneacetic acid, kinetin 0.08 parts, 02 parts of aspartate, 0.22 parts of conditioner, 2.5 parts of cosolvent, 2 parts of dispersant, 1.6 parts of compatibilizer, 1.3 parts of stabilizer, 2 parts of antibacterial agent, 3500 parts ...

Embodiment 2

[0092] A culture medium for dendrobium candidum tissue culture seedlings with improved alkaloids, comprising the following raw materials in parts by weight: 162 parts of apple juice, 2.6 parts of citric acid, 0.02 parts of sodium o-benzoylsulfonimide, and 1.5 parts of tea polyphenols 2.6 parts of potassium salts, 1.5 parts of phosphorus salts, 1.2 parts of nitrogen salts, 0.8 parts of calcium salts, 0.7 parts of magnesium salts, 0.4 parts of iodine salts, 0.2 parts of boron salts, 0.2 parts of molybdenum salts, 0.3 parts of zinc salts, and 0.1 parts of manganese salts 0.1 part of copper salt, 0.1 part of iron salt, 0.12 part of vitamin, 0.1 part of amino acid, 0.08 part of indole butyric acid, 0.08 part of gibberellin, 0.07 part of 6-benzyl adenine, 0.08 part of naphthaleneacetic acid, kinetin 0.05 parts, 0.14 parts of aspartate, 0.19 parts of conditioner, 1.6 parts of cosolvent, 1.4 parts of dispersant, 1.3 parts of compatibilizer, 1 part of stabilizer, 1.4 parts of antibacter...

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Abstract

The invention discloses a dendrobium candidum tissue culture seedling culture medium for increasing alkaloid and a preparing method thereof. The dendrobium candidum tissue culture seedling culture medium is prepared from apple juice, citric acid, saccharin soluble hydrate, tea polyphenol, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodate, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, ferric salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6-benzylaminopurine, naphthylacetic acid, kinetin, asparagine, conditioner, cosolvent, a dispersing agent, compatilizer, stabilizer, an antibacterial agent and water. According to the dendrobium candidum tissue culture seedling culture medium, the obtained seedling is good in quality and high in survival rate, and can effectively improve the content of alkaloid, polysaccharide and amino acid.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of dendrobium candidum planting, and in particular relates to a culture medium for tissue culture seedlings of dendrobium candidum with enhanced alkaloids and a preparation method thereof. [0003] 【Background technique】 [0004] Dendrobium officinale is a plant of the orchid family. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. The main active ingredients are alkaloids, polysaccharides, and amino acids. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. It grows in the shade of cliffs, and its roots do not go into the soil. Fat, lower blood sugar, inhibit tumor, brighten eyes, nourish skin and prolong life. "Chinese Pharmacy Dictionary" states that Dendrobium candidum "specially treats the gas and liquid of the lung and stomach, the gas and liquid are prosperous, and the kidney water is self-generated." It is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 许焕明
Owner 许焕明
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