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A kind of photoelectrochemical sensor and its preparation and application

A photoelectrochemical and sensor technology, applied in the detection of thrombin in medicines and cosmetics, human serum, and the preparation of photoelectrochemical sensors, can solve the problems of unsuitable detection of thrombin, poor biocompatibility of toxic heavy metals due to photocorrosion, etc. Effects of good photocurrent response, improved photocurrent response, and efficient electron injection capability

Active Publication Date: 2018-12-18
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a photoelectrochemical sensor and its preparation and application to solve the problems of traditional photoelectrochemical sensors, such as photocorrosion and poor biocompatibility of toxic heavy metals, which are not suitable for the detection of thrombin.

Method used

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  • A kind of photoelectrochemical sensor and its preparation and application
  • A kind of photoelectrochemical sensor and its preparation and application
  • A kind of photoelectrochemical sensor and its preparation and application

Examples

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Embodiment 1

[0045] A kind of preparation method of photoelectrochemical sensor, such as figure 1 As shown, it mainly includes the following steps:

[0046] (1) Synthesis of carbon quantum dots

[0047] Dissolve 0.735 g of citric acid and 235 μL of ethylenediamine in 7 mL of ultrapure water, and stir vigorously for 10 min. Then the mixed solution was transferred to a 13 mL polytetrafluoroethylene-lined stainless steel autoclave, and the autoclave was placed in an oven at 200° C. for 5 h. After 5 hours, the stainless steel autoclave was taken out, and placed at room temperature to cool naturally. Then the dark brown solution was transferred into a dialysis bag with a molecular cut-off (MWCO) of 1000 and dialyzed for 2-3 days. Concentrate the obtained dialysate with a rotary evaporator, then place the obtained concentrated solution in a vacuum oven at 60° C. for 48 hours, and finally grind the obtained brown-black solid to obtain carbon quantum dot powder. The carbon quantum dot solution...

Embodiment 2

[0065] (1) Synthesis of carbon quantum dots

[0066]Dissolve 0.735 g of citric acid and 235 μL of ethylenediamine in 8 mL of ultrapure water, and stir vigorously for 10 min. Then the mixed solution was transferred to a 13 mL polytetrafluoroethylene-lined stainless steel autoclave, and the autoclave was placed in an oven at 220° C. for 3 h. After 3 hours, the stainless steel autoclave was taken out, and placed at room temperature to cool naturally. Then the dark brown solution was transferred into a dialysis bag with a molecular cut-off (MWCO) of 1000 and dialyzed for 2-3 days. Concentrate the obtained dialysate with a rotary evaporator, then place the obtained concentrated solution in a vacuum oven at 60° C. for 48 hours, and finally grind the obtained brown-black solid to obtain carbon quantum dot powder.

[0067] (2) Synthesis of titanium dioxide nanoparticles

[0068] Take 9 mL of tetrabutyl titanate solution and add it to 40 mL of absolute ethanol, mix and stir evenly, ...

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Abstract

The invention relates to a photoelectrochemical sensor, and preparation and application thereof. A preparation method for the photoelectrochemical sensor comprises the following steps: S1, pretreating ITO electro-conductive glass; S2, adding TiO2 nanoparticles onto the ITO electro-conductive glass drop by drop to prepare an ITO / TiO2 electrode; S3, soaking the electrode in a carbon quantum dot solution for 10 h so as to obtain an ITO / TiO2 / CODs electrode; S4, adding chitosan onto the electrode drop by drop so as to obtain an ITO / TiO2 / CODs / CS electrode; S5, adding a glutaraldehyde solution onto the electrode drop by drop; and S6, dropwise adding thrombin aptamer drops, carrying out a reaction at 4 DEG C under a wet condition for 10 to 15 h, continuing adding bovine serum albumin onto the electrode drop by drop after leaching and blow-drying, and carrying out leaching and blow-drying again. The prepared photoelectrochemical sensor can be used for detection of thrombin. According to the invention, the carbon quantum dots with a wide visible light absorption range, good luminosity and high-efficiency electron injection capability are used as a photosensitizer for titanium dioxide, so the obtained composite structure has good response to photoelectric current under visible light. The prepared photoelectrochemical sensor has the advantages of simple operation, low cost, environment friendliness, high sensitivity and good selectivity.

Description

technical field [0001] The invention belongs to the new field of new functional materials and biosensing technology, and relates to the preparation of a photoelectrochemical sensor based on carbon quantum dots and titanium dioxide composite materials, and its application to the detection of thrombin in human serum, medicines and cosmetics. Background technique [0002] The photoelectrochemical process refers to the photoelectric conversion process of photosensitive materials through electronic excitation and charge transfer under the action of light. As an independent integrated detection device, electrochemical biosensors have had an increasingly important impact on the biochemical and medical fields. With the rapid development of nanotechnology and material chemistry, a new generation of photoelectrochemical (PEC) biosensing has been developed on the basis of the combination of photoelectrochemical processes and electrochemical biosensors, thus providing a great opportunit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 高文华潘嘉宏陈耀文张歆林月娟张晓珊
Owner SHANTOU UNIV
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