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Rapid propagation method of lauraceae machilus

A zhennan, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long cultivation and reproduction time, low survival rate, etc., and achieve good formula effect, high germination rate, and vigorous plant growth. Effect

Inactive Publication Date: 2017-03-15
YIBIN YUNCHEN ARBOR GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are certain differences between the above-mentioned prior art golden nanmu and Zhennan
In addition, the seeds are cultivated first, and then the tissue culture is carried out, and the cultivation and reproduction time is longer; the medium used makes the survival rate lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A rapid propagation method of Zhennan, characterized in that: comprising the following method steps:

[0034] A. Selection and sterilization of explants

[0035] The buds on the tender stems of Zhennan were selected as the culture material, washed with tap water first, then rinsed with washing powder solution, rinsed with tap water, then placed in 75% ethanol solution, and then sterilized by 0.1% mercuric chloride Afterwards, rinse with sterile water, dry the water with sterile absorbent paper, cut off the discolored part of the explant, and place it in a sterile petri dish for later use;

[0036] B. Germination of buds

[0037]Inoculate the sterilized buds on WPM+NAA1.0mg / L+6-BA2.0mg / L+PVP6mg / L medium, the pH value is 5.8, the culture temperature is 23°C, and the light is 12 hours a day. The strength is 1700Lx, after inoculation, the buds germinate, and then form small seedlings with leaves;

[0038] C. Callus induction

[0039] Take the buds in the initial inducti...

Embodiment 2

[0047] A rapid propagation method of Zhennan, characterized in that: comprising the following method steps:

[0048] A. Selection and sterilization of explants

[0049] The buds on the tender stems of Zhennan were selected as the culture material, washed with tap water first, then rinsed with washing powder solution, rinsed with tap water, then placed in 75% ethanol solution, and then sterilized by 0.1% mercuric chloride Afterwards, rinse with sterile water, dry the water with sterile absorbent paper, cut off the discolored part of the explant, and place it in a sterile petri dish for later use;

[0050] B. Germination of buds

[0051] Inoculate the sterilized buds on WPM+NAA1.0mg / L+6-BA2.0mg / L+PVP6mg / L medium, the pH value is 6.0, the culture temperature is 27°C, and the light is 12 hours a day. The strength is 1700Lx, after inoculation, the buds germinate, and then form small seedlings with leaves;

[0052] C. Callus induction

[0053] Take the buds in the initial induct...

Embodiment 3

[0061] A rapid propagation method of Zhennan, characterized in that: comprising the following method steps:

[0062] A. Selection and sterilization of explants

[0063] The buds on the tender stems of Zhennan were selected as the culture material, washed with tap water first, then rinsed with washing powder solution, rinsed with tap water, then placed in 75% ethanol solution, and then sterilized by 0.1% mercuric chloride Afterwards, rinse with sterile water, dry the water with sterile absorbent paper, cut off the discolored part of the explant, and place it in a sterile petri dish for later use;

[0064] B. Germination of buds

[0065] Inoculate the sterilized buds on WPM+NAA1.0mg / L+6-BA2.0mg / L+PVP6mg / L medium, the pH value is 5.9, the culture temperature is 25°C, and the light is 12 hours a day. The strength is 1700Lx, after inoculation, the buds germinate, and then form small seedlings with leaves;

[0066] C. Callus induction

[0067] Take the buds in the initial induct...

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PUM

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Abstract

The invention relates to a rapid propagation method of lauraceae machilus, and belongs to the technical field of tree propagation. The rapid propagation method comprises the steps of explant selection and sterilization, bud sprouting, callus induction, differentiation and enrichment culture, rooting culture and seedling exercising and transplanting. The rapid propagation method of the lauraceae machilus is very suitable for large-scale production and has the advantages of being good in formula effect, high in sprouting rate, short in culture time, vigorous in plant growth and high in operability and application value.

Description

technical field [0001] The invention relates to a rapid propagation method of arbor, more specifically, relates to a rapid propagation method of Phoebe zhennan, which belongs to the technical field of tree propagation. Background technique [0002] Zhennan is a large evergreen tree of Lauraceae, which belongs to the second-class national protected plant and is also a special tree species in China, and has extremely high economic value. Zhennan wood is excellent, with straight texture and fine structure, not easy to deform and crack, and has aroma. It is a precious material for construction and furniture. Its wood and branches and leaves contain aromatic oil, which can be distilled to obtain nanmu oil, which is a high-grade spice. At the same time, this species is a tall tree with beautiful posture, straight trunk and leaves that do not shed all year round. It is a famous garden ornamental and urban greening tree species. [0003] Zhennan is extremely precious, and its grow...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗玉玲
Owner YIBIN YUNCHEN ARBOR GARDEN
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