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Structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed

A kind of influenza vaccine, ad5-m2m technology, applied in the field of vaccine preparation, can solve the problem that inactivated vaccines cannot cross-protect different branches of the same subtype, cannot induce cellular immune response and mucosal immune response, and the speed of vaccine update cannot keep up with the speed of virus mutation and other issues, to achieve the effect of great application value, low cost, and good immunogenicity

Inactive Publication Date: 2017-03-08
SOUTH CHINA AGRI UNIV +2
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Problems solved by technology

[0002] At present, influenza vaccines are mainly used in the prevention of influenza. The vaccine for avian influenza is mainly an inactivated whole virus vaccine. This vaccine is safe and well tolerated, and can induce a high humoral immune response, but cannot induce effective cellular immune response and mucosal immune response. Immune response, and the incidence of adverse reactions during the vaccination process is also high, affecting the egg production rate and meat quality of poultry; at the same time, due to the continuous variation and recombination of avian influenza viruses, it is common that inactivated vaccines cannot cross-protect different branches of the same subtype Phenomenon, so that the update speed of the vaccine cannot keep up with the mutation speed of the virus

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  • Structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed
  • Structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed
  • Structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed

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Embodiment Construction

[0031] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0032] see Figure 1-5 A method for constructing and preparing Ad5-M2m as a universal influenza vaccine virus is realized by modifying, concatenating and optimizing the conserved antigen M2 of influenza virus, and constructing Ad5-M2m, comprising the following steps:

[0033] 1) Modification, concatenation and optimization of the main conserved immunogen M2 of influenza virus:

[0034] Comparative analysis and antigenic site prediction analysis of M2 full-length sequences from H1N1, H3N2, and H5N1 subtype influenza viruses, site transformation and modification, after tandem and codon optimization, a tandem M2m gene was formed, which was handed over to The reagent company synthesized the gene sequence, and constructed the recombinant adenovirus Ad5-M2m carrying the tandem M2m gene through gene recombination technology; and then determined whether t...

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Abstract

The invention discloses a structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed; the Ad5-M2m recombinant adenovirus is prepared as a general influenza vaccine virus seed; the preparation method includes steps of structuring multiple flu subtype conservative immunogen M2m recombinant adenovirus and establishing the virus seed; inoculating and cultivating Ad5-M2m; harvesting Ad5-M2m; preliminary immunizing of Ad5-M2m, and so on. The Ad5-M2m used as general influenza vaccine virus seed prepared by the invention is wide in antigen spectrum, stable in quality, high in purity, good in safety, low in cost and others; the method is applicable to large-scale production and has big application value.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, in particular to a method for constructing and preparing Ad5-M2m recombinant adenovirus as a universal influenza vaccine virus seed by using a genetic engineering method. Background technique [0002] At present, influenza vaccines are mainly used in the prevention of influenza. The vaccine for avian influenza is mainly an inactivated whole virus vaccine. This vaccine is safe and well tolerated, and can induce a high humoral immune response, but cannot induce effective cellular immune response and mucosal immune response. Immune response, and the incidence of adverse reactions during the vaccination process is also high, which affects the egg production rate and meat quality of poultry; at the same time, due to the continuous variation and recombination of avian influenza viruses, it is common that inactivated vaccines cannot cross-protect different branches of the same subtype The ph...

Claims

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Application Information

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IPC IPC(8): C12N15/861C12N15/44A61K39/145A61P31/16
CPCC12N15/86A61K39/12A61K2039/5256A61K2039/552C07K14/005C12N2710/10043C12N2760/16122C12N2760/16134
Inventor 罗琴芳陈瑞爱黄文科唐满华汤钦冯立强潘蔚绮陈凌
Owner SOUTH CHINA AGRI UNIV
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