Target region enrichment sequencing method for 165 genes

Abstract

The invention provides a target region enrichment sequencing method for 165 genes. According to the method, 165 group-entering genes are screened according to the cancer genome and medicine data of TCGA, NCCN, FDA and the like. The 165 genes comprise detection genes recommended by NCCN, target genes corresponding to target medicines and important clinical medicines approved by FDA, genes influencing the medication effect of the target medicines, and genes influencing the chemical drug sensibility and toxic and side effects. According to the method, the exon regions of the 165 genes are enriched at once through the probe capturing technology, the multi-gene and multi-target parallel depth high-throughput sequencing is realized, the various variation types (such as point mutation, deletion, insertion, fusion and copy number amplification) of the genes can be simultaneously detected, and the detection depth and accuracy are superior to those of the prior art.

Description

technical field [0001] The invention relates to the field of genetic engineering and biotechnology detection, in particular to a method for enriching target regions of 165 genes, which is used for multi-target parallel high-throughput sequencing of circulating tumor DNA (ctDNA), and simultaneously checks multiple genes Variation types, such as mutations, deletions, insertions, fusions, and copy number amplifications, outperform existing technologies in depth and breadth of detection. Background technique [0002] Circulating DNA is a kind of extracellular DNA in a cell-free state, which exists in body fluids such as blood, synovial fluid, and cerebrospinal fluid. It is mainly composed of single-stranded or double-stranded DNA and a mixture of single-stranded and double-stranded DNA. Complex or free DNA exists in two forms. As a new tumor marker, circulating DNA will play an important role in the diagnosis, treatment and prognosis of tumors, especially for some tumors that d...

AI Technical Summary

Problems solved by technology

In addition to whole genome sequencing and whole exome sequencing, these methods do not require known mutation information, but are expensive

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