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Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof

A technology of molecular markers and shimeji mushrooms, applied in the fields of application, fungi, botanical equipment and methods, etc., can solve the problems of short stipe and single variety, and achieve the effect of long fresh-keeping period, beautiful flower shape and rich variety

Inactive Publication Date: 2017-02-22
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a pure white shimeji mushroom strain, which is used to solve the problem that the stipe of the current shiji mushroom varieties is short and the variety is single

Method used

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  • Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof
  • Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof
  • Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Polyspore selfing to obtain Finc-W-6

[0057] The strain Finc-W-6 was obtained by polyspore self-breeding method.

[0058] Specific steps are as follows:

[0059] 1) The parent strain H-W was purchased from the market;

[0060] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;

[0061] 3) Isolation of basidiospores: the parent strain H-W was placed in a petri dish, and after 24 hours, the spores falling on the petri dish were collected, diluted to an appropriate concentration in sterile water, and spread on a PDA medium plate. Cultivate at 23°C for 10 days, and inoculate the spores on the PDA inclined test tubes with inoculation loops after germination. The tubes were incubated at 23°C for 12 days, after which hyphae were isolated from the tubes and observed under a microscope for the formation of lock junctions. Store stra...

Embodiment 2

[0065] Example 2 Antagonism experiment

[0066] The Finc-W-6 of the present invention and the parent bacterial strain H-W are test materials, and the medium used is PDA medium.

[0067] Finc-W-6 and the parental strain H-W were inoculated and cultured on the plate medium respectively. The distance between the inoculation points was 2.5 cm, and they were cultured at a constant temperature of 23°C for 18-20 days. Please refer to Table 1 for the results of this experiment.

[0068] Table 1 Antagonism test among different pure white jiji mushroom strains

[0069] Variety H-W Finc-W-6 antagonistic

[0070] Such as Figure 1-2 As shown, there is an obvious antagonistic line between the junctions of the confrontation culture colonies, which shows that Finc-W-6 is a pure white jiji mushroom strain different from the parental strain H-W.

Embodiment 3

[0071] Example 3 Cultivation experiment

[0072] Finc-W-6 of the present invention and parent bacterial strain H-W are respectively cultivated and cultivated, and 4 bacterial ages are set from 70 days to 85 days, and every increase of 5 days is a bacterial age, and each bacterial age is 96 bottles, and then cultivated in small batches The results were summarized and compared, and the advantages and characteristics of the bacterial strain Finc-W-6 of the present invention were summarized.

[0073] The culture medium of this embodiment participates in the cultivar culture medium, and will not be repeated here.

[0074] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.

[0075] Cultivation: Cultivate for 70-85 days at a temperature of 18-23°C, a relative air humidity of 65-75%, and a carbon dioxide concentration of 2000-3500ppm;

[0076] Cultivation: The fruiting temperature is set at 13°C-15°C, the relative air humidity is 93%-100%, and the carbon dioxide concentra...

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Abstract

The invention belongs to the technical field of edible fungi and particularly relates to a pure white hypsizigus marmoreus strain, and a molecular marker, a specific primer pair and an application thereof. The pure white hypsizigus marmoreus strain provided by the invention has a name of Finc-W-6, belongs to hypsizigus marmoreus, is collected in China General Microbiological Culture Collection Center (CGMCC) with an address of 3#, yard 1#, west beichen road, chaoyang district, Beijing, and has a collection number of CGMCC No.12523 and a collection date of June, 30th, 2016. According to the technical scheme provided by the pure white hypsizigus marmoreus strain, and the molecular marker, the specific primer pair and the application thereof, the obtained long-stem pure white hypsizigus marmoreus meets diversified demands of the market and is short in cultivation period, relatively concentrative in fruiting time, high in unit yield, beautiful in shape, long in refreshing time, very suitable for large-area planting promotion and wide in market prospect.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a pure white mushroom strain, a molecular marker, a pair of specific primers and an application thereof. Background technique [0002] Hypsizygus marmoreus (Latin name Hypsizygus marmoreus), which belongs to Basidiomycotina, Laminomycetes, Agaricaceae, White Mushrooms, Liphyllum family, Amygium genus, because it has a unique crab flavor, it is also called It is crab-flavored mushroom. At present, there are two strains of light gray and pure white. The white strain is also called "white jade mushroom" and "yulong mushroom". [0003] The initial artificial cultivation began in Japan around 1978. It is delicious, crisp and tender, and rich in nutrition. The product is also full of fragrance. True jiji mushroom is rich in vitamins and 17 kinds of amino acids. The β-1,3-D glucan extracted from the fruiting body has high anti-tumor activity. It is a low-calorie, low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C12Q1/68C12N15/11C12R1/645
CPCA01G18/00C12N1/14C12Q1/6895C12Q2600/156C12N1/145C12R2001/645
Inventor 韩成玲钱仙云张文宏吴慧敏
Owner SHANGHAI FINC BIO TECH INC
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