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Method for cultivating morchella single-spore strains by adopting 'artificial pollination'

A technology of artificial pollination and hickory chick, which is applied in cultivation, plant cultivation, mushroom cultivation, etc. It can solve the problems of waste, unfavorable Y-shaped hyphae to produce small conidia, etc., so as to increase production, promote high-efficiency formation, and improve cultivation efficiency effect

Active Publication Date: 2017-02-22
四川省食用菌研究所
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

After the nutrient bag was placed to supply the mycelia with nutrients, both the X-type and Y-type mycelium obtained the nutrient supply, which was not conducive to the Y-type mycelia to continue to produce microconidia efficiently, and at the same time, they were absorbed and stored in the Y-type mycelia. The nutrients in the cells are meaningless for fruiting, but it takes away the nutrients that should be absorbed by the X-type hyphae, which is also a waste

Method used

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  • Method for cultivating morchella single-spore strains by adopting 'artificial pollination'
  • Method for cultivating morchella single-spore strains by adopting 'artificial pollination'

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Embodiment 1

[0059] Time: From September 2014

[0060] Location: Shuangliu County, Chengdu City, Sichuan Province

[0061] Species preparation: Take the X-type strain and the Y-type strain of Morchella importuna M3#, and insert them into the strain bottle respectively. The seed bottle is a glass bottle with a capacity of 800 ml and a ratio of height to diameter of 1.5:1, containing 3 / 4 volume of bran culture medium. The bran medium is prepared by spraying water into the dry bran to a humidity of 60%, and then gradually adding quicklime to adjust the pH value to 8.4. The strain bottle was cultured at 18°C ​​for 17 days, so that the morel hyphae spread into most of the bran medium, and X-type strains and Y-type strains were obtained respectively.

[0062] Sowing of X-type strains: the cultivated land adopts the method of high-box ridge cultivation and double-furrow drilling to make a mother fungus bed with a height of 30 cm and a width of 100 cm, and the width of the furrow between the rid...

Embodiment 2

[0068] Time: Beginning in October 2015

[0069] Location: Xindu District, Chengdu City, Sichuan Province

[0070] Breed preparation: Take the X-type strain and the Y-type strain of Morchella sextelata M0#, and put them into the strain bottle respectively. The seed bottle is a glass bottle with a capacity of 800 ml and a ratio of height to diameter of 2:1, containing 3 / 4 volume of bran culture medium. The bran medium is prepared by spraying water into the dry bran to a humidity of 60%, and then gradually adding quicklime to adjust the pH value to 8.1. The strain bottle was cultured at 20°C for 14 days, so that the morel hyphae spread into most of the bran medium, and the X-type strains and Y-type strains were obtained respectively.

[0071]Sowing of X-type strains: the cultivated land adopts the method of high-box ridge cultivation and double-furrow drilling to make a mother fungus bed with a height of 35 cm and a width of 120 cm, and the width of the furrow between the ridge...

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Abstract

The invention belongs to the field of morchella cultivation and relates to a method for cultivating morchella single-spore strains by adopting 'artificial pollination'. The method promotes efficient formation of XY types of hyphae, facilitates final fruiting and plays the effects of improving the cultivation efficiency and increasing the yield. The cultivating method comprises the steps that (A) strain preparation is performed; (B) an X type of strains are sowed; (C) a Y type of spores produce pollen; (D) artificial pollination is performed; (E) management and harvesting are performed and the like. According to the method, a concept and a method of 'artificial pollination' are introduce to the edible fungi cultivation field, the Y type of microgonidium spores is fertilized to the X type of hyphae, strain hybridization is achieved, and the novel cultivation method is provided for morchella hybridization and crossbreeding.

Description

technical field [0001] The invention belongs to the field of hickory chick cultivation, and relates to a method for cultivating hickory chick monospore strains by "artificial pollination". Background technique [0002] Morels are a delicious edible fungus in the Ascomycota family. Morchella is rich in nutrition and has the nourishing effect of nourishing the stomach and protecting the liver. It is widely welcomed by consumers, and the supply exceeds demand in the mushroom market. With the rapid growth of the market demand for morels, it has been unable to meet the demand by collecting wild morels. Therefore, there have been a batch of techniques for artificially cultivating hickory chicks in recent years, such as the cultivation methods described in patents and application publication numbers CN103782802A, CN103583240A, and CN101053302 of authorized announcement numbers CN103583232B, CN103202177B, and CN101628834B. The fruiting of Morchella depends on its sexual reproducti...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H1/02
CPCA01G18/00A01H1/02
Inventor 谭昊甘炳成彭卫红黄忠乾唐杰苗人云刘天海李小林
Owner 四川省食用菌研究所
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