Environment-friendly transparent dewaxing liquid, preparation method thereof and application of environment-friendly transparent dewaxing liquid in preparing pathological section

Abstract

The invention belongs to the field of pathological detection reagents, and particularly relates to environment-friendly transparent dewaxing liquid, a preparation method thereof and application of the environment-friendly transparent dewaxing liquid in preparing a pathological section. The environment-friendly transparent dewaxing liquid is prepared from the following raw materials by a mass ratio: 20-60% of white oil, 12-45% of nonane, 6-35% of octane, 5-20% of heptane, 4-25% of castor oil, 3-18% of pentanol and 2-9% of hexanol. The dewaxing liquid disclosed by the invention is simple in formula and reasonable in collocation, and can serve as a clarifier and a dewaxing agent used during preparation of the pathological section, so that a pathological section process is completely free from a xylene reagent.

Description

Technical field [0001] The invention belongs to the field of pathological detection reagents, and particularly relates to an environmentally-friendly transparent dewaxing solution, a preparation method thereof, and an application thereof in pathological section preparation. Background technique [0002] According to reports, new cancer patients in China account for more than 20% of the global number of new cancers each year. Among them, lung cancer, liver cancer, gastric cancer, esophageal cancer, colorectal cancer, cervical cancer, breast cancer, and nasopharyngeal cancer deaths are approximately It accounts for more than 80% of all cancer deaths in China. Early diagnosis and early treatment of cancer patients is the key to improving the survival rate of patients. At present, the early diagnosis of cancer mainly depends on the staining results of pathological sections, so it is very important that the staining technology of pathological sections is fast and efficient. The basi...

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Problems solved by technology

However, xylene also has fatal disadvantages: 1) It has strong contractility to tissues, and it is easy to make tissues hard and brittle, resulting in different thicknesses, and it is difficult to grasp the transparency and dewaxing time of pathological tissues in different parts. The dewaxing step causes the film to fall off; 2) Volatile and toxic to a certain extent, it has toxic effects on the human nervous system, respiratory system, hematopoietic system, immune system and reproductive system, and is one of the mutagenic and carcinogenic substances. Long-term exposure Symptoms such as neurasthenia syndrome and decreased immunity of the body will occur, which is not conducive to the health of the staff and environmental protection. Especially during the operation of rapid paraffin film production, xylene needs to be heated to accelerate volatilization, but this not only pollutes the environment but also Harmful to the health of the operator

[0005] Zhan Yanping and others disclosed the use of turpentine instead of xylene as a transparent agent, but the transparency and dewaxing effect of pure turpentine is far less than that of xylene, and the solution will become viscous after a long period of use [Zhan Yanping, Zhang Yun. Turpentine instead of xylene is transparent Discussion on tablet preparation[J]. Journal of Practical Medical Technology. 2007, 14(16): 2256];

[0006] Wang Qingxia and others disclosed the use of stearic acid and paraffin mixed liquid instead of xylene as a transparent agent, and achieved good results, but there are also the following defects: when the temperature is lower than 66 ° C, the stearic acid is not completely dissolved , fine particles suspended in paraffin, these particles appear as opaque blurred areas in the section, affecting observation under the microscope; and incomplete dissolution will also cause potential loose gaps between the section and the glass slide, resulting in poor adhesion of the section Firm, easy to peel off [Zhou Dao, Gu Ping, Yan Junning, Discussion on stearic acid paraffin mixed solution instead of xylene clearing agent for film production[J], Journal of Diagnostic Pathology, 2006, 13(1): 75-76,19 ; Wang Qingxia, An Huibo, Liu Zhengyan, etc. Application of stearic acid and tissue dewaxed transparent liquid instead of xylene in pathological film production[J]. Journal of Clinical and Experimental Pathology. 2009, 25(04):442—443 】;

It can completely replace xylene and toluene to clear and dewax pathological sections, but because of its high price, its scope of use is severely limited

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