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Active amino-modified substrate for microarray and preparation method of active amino-modified substrate

A technology of amino modification and microarray, which is applied in the fields of medicine and biology to solve the problems of low sensitivity, improved fixation rate and poor stability

Inactive Publication Date: 2017-01-25
重庆高圣生物医药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the deficiency that the existing gene chips use glass substrates or simply silanized glass substrates as carriers, and provide a microarray with active amino modified substrates and a preparation method thereof

Method used

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  • Active amino-modified substrate for microarray and preparation method of active amino-modified substrate

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Embodiment 1

[0035] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:

[0036] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid:hydrogen peroxide = 7:3 for 13 h, and remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Rinse twice with deionized water, each time for 5 minutes, and finally spin dry to obtain a pretreated glass substrate;

[0037] Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 1.0% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the residual 3-aminopropyldiethoxysilane solution is cleaned with 60% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;

[0038] Step (3), poly-lysine coat...

Embodiment 2

[0040] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:

[0041] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid: hydrogen peroxide = 7:3 for 18 h, remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Wash with deionized water 5 times, each time for 5 minutes, and finally spin dry to obtain the pretreated glass substrate;

[0042]Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 3.0% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the remaining 3-aminopropyldiethoxysilane solution is cleaned with 80% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;

[0043] Step (3), poly-lysine coat...

Embodiment 3

[0045] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:

[0046] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid: hydrogen peroxide = 7:3 for 15 h, and remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Wash with deionized water for 3 times, each time for 4min, and finally spin dry to obtain the pretreated glass substrate;

[0047] Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 2.5% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the residual 3-aminopropyldiethoxysilane solution is cleaned with 70% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;

[0048] Step (3), poly-lysine c...

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Abstract

The invention relates to an active amino-modified substrate for a microarray and a preparation method of the active amino-modified substrate and belongs to the technical field of medical science and biology. The biological chip substrate is characterized in that amino silanization treatment is performed on the surface of a glass substrate, and polylysine coating is performed on the obtained glass substrate with rich amino on the surface to obtain the active amino-modified glass substrate. The active amino-modified substrate has the advantages that due to the fact preprocessing is performed by using concentrated sulfuric acid and hydrogen peroxide according to optimal proportion, the problem that a common substrate has many impurities is solved, polylysine coating is performed after the amino silanization, the dual combined effect of static absorption and chemical coupling is formed, the fixing rate of the substrate to a probe is evidently increased, and the problems that the substrate is low in sensitivity and poor in stability are solved; the substrate is a biological chip probe vector with excellent performance and quality and is widely applicable to biological chip preparation.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a biochip substrate and a preparation method thereof, in particular to an active amino-modified substrate for a microarray and a preparation method thereof. Background technique [0002] Since the 1990s, the connotation covered by the gene chip technology has been continuously expanded, and the technical methods have been continuously improved. Because it can detect the expression levels of tens of thousands of genes at the same time, it can help us study the molecular mechanisms and biological pathways of diseases, and has become a very important research tool in the field of biochemistry and molecular biology. The substrate is the basis of the gene chip, and the preparation of the substrate is a very important link in the development of the gene chip. Currently commonly used gene chips are simple silanized substrates, which are modified by silanization on the surfac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L2300/0819B01L2300/0822B01L2300/12
Inventor 周勇申友锋
Owner 重庆高圣生物医药有限责任公司
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