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A kind of assay method of total polyphenol content in litter

A determination method and litter technology, applied in the field of determination of polyphenol content, can solve the problems of unsuitable quantitative analysis, high cost, complicated pretreatment steps, etc., and achieve the effects of saving experimental cost, less destructiveness, and shortening measurement time.

Inactive Publication Date: 2018-07-06
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gas-liquid chromatography for the separation and determination of plant phenolic substances has the advantages of fast speed and high sensitivity, but derivatization treatment is required, the pretreatment steps are cumbersome, and the cost is expensive, so it is not suitable for quantitative analysis of large-scale samples

Method used

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  • A kind of assay method of total polyphenol content in litter
  • A kind of assay method of total polyphenol content in litter
  • A kind of assay method of total polyphenol content in litter

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for determining the content of total polyphenols in litter, using ethanol solution for pretreatment, using Folin–Ciocalteu reagent to quantitatively oxidize the substance to be tested, and finally using a multifunctional microplate reader for quantitative analysis. Consists of the following steps:

[0023] (1) After the litter is removed from impurities, it is quickly dried and pulverized in an oven at 55°C, and passed through a 70-mesh sieve;

[0024] (2) Weigh 0.08g of the powder sieved in step (1), add 0.8mL of ethanol solution with a volume fraction of 70%, mix well, seal it and put it in a constant temperature oscillation box, shake it at 250rpm room temperature for 3 hours, and then mix Incubate in the dark for 16 hours;

[0025] (3) Place the cultured mixture in a high-speed centrifuge, centrifuge at 10,000 rpm for 15 minutes, transfer the supernatant to a new test tube, seal it at 4°C and store it away from light until use, and perform the operation of...

Embodiment 2

[0031] A method for determining the content of total polyphenols in litter, using ethanol solution for pretreatment, using Folin–Ciocalteu reagent to quantitatively oxidize the substance to be tested, and finally using a multifunctional microplate reader for quantitative analysis. Consists of the following steps:

[0032] (1) After the litter is removed from impurities, it is quickly dried and pulverized in an oven at 60°C, and passed through a 70-mesh sieve;

[0033] (2) Weigh 0.09g of the powder sieved in step (1), add 0.9mL of ethanol solution with a volume fraction of 80%, mix well, seal it and place it in a constant temperature oscillation box, shake it at 220rpm room temperature for 4 hours, and then mix Incubate in the dark for 14 hours;

[0034] (3) Place the cultured mixture in a high-speed centrifuge, centrifuge at 11,000 rpm for 16 minutes, transfer the supernatant to a new test tube, seal it at 4°C and store it in the dark for later use, and perform the step ( 4)...

Embodiment 3

[0040] A method for determining the content of total polyphenols in litter, using ethanol solution for pretreatment, using Folin–Ciocalteu reagent to quantitatively oxidize the substance to be tested, and finally using a multifunctional microplate reader for quantitative analysis. Consists of the following steps:

[0041] (1) After the litter is removed from impurities, it is quickly dried and pulverized in an oven at 65°C, and passed through an 80-mesh sieve;

[0042] (2) Weigh 0.1 g of the powder sieved in step (1), add 1 mL of ethanol solution with a volume fraction of 95%, mix well, seal it and place it in a constant temperature shaking box, and shake it at 300 rpm for 3.5 hours at room temperature, then the mixture is kept away from the Light culture for 12h;

[0043] (3) Place the cultured mixture in a high-speed centrifuge, centrifuge at 12,000 rpm for 12 minutes, transfer the supernatant to a new test tube, seal it at 4°C and store it in the dark for later use, and pe...

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Abstract

The invention relates to a measuring method of polyphenol content, in particular to a measuring method of total polyphenol content in litter. Pretreatment is conducted using ethanol solution, Folin-Ciocalteu reagent serves as oxidizing agent to quantitatively oxidize the measured substance after centrifugalization, and finally quantitative analysis is conducted using a multi-functional enzyme mark instrument. According to the measuring method of total polyphenol content in litter, total polyphenol is optimized, particularly the measuring condition of total polyphenol content in litter, accuracy is improved, measuring time is reduced, cost is saved, and the method is suitable for quantitative detection of large-scale sample.

Description

technical field [0001] The invention relates to a method for determining the content of polyphenols, in particular to a method for determining the content of total polyphenols in litter. Background technique [0002] Plant tannins, also known as plant polyphenols, are a class of polyphenolic compounds that widely exist in plants. The content in vascular plants is second only to cellulose, hemicellulose and lignin. It mainly exists in the bark and root of plants. , leaves and fruits, the content can reach 20%. Polyphenols in litters are difficult to decompose polymers, and their content usually significantly affects the decomposition rate of litters. The higher the content of polyphenols, the slower the decomposition of litters. Polyphenols in litter play an important role in the co-evolution of plants and the environment. Litters such as leaves and roots rich in polyphenols enter the soil, which will affect the microbial activity in the soil, the availability of mineral ele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/543
Inventor 吕艳娜王玲蒲高忠杜京京唐金宝陈燕刘壮壮
Owner WEIFANG MEDICAL UNIV
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