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A kind of enterobacteriaceae bacterial strain with phosphate-solubilizing ability and application thereof

A technology of Enterobacteriaceae and strains, applied in the field of insoluble phosphate biodegradation, can solve problems such as unsatisfactory effect of phosphate-solubilizing bacteria, and achieve the effects of alleviating phosphorus crisis, increasing yield and good tolerance

Active Publication Date: 2019-10-08
YUNNAN PHOSPHATE CHEM GROUP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent has a good effect on degrading phosphorus salts in soil, due to the large variety and high content of insoluble inorganic phosphates in the scrubbing tailings, the effect of the phosphorus-dissolving bacteria on the degradation of the scrubbing tailings is not ideal.

Method used

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  • A kind of enterobacteriaceae bacterial strain with phosphate-solubilizing ability and application thereof
  • A kind of enterobacteriaceae bacterial strain with phosphate-solubilizing ability and application thereof
  • A kind of enterobacteriaceae bacterial strain with phosphate-solubilizing ability and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The acquisition of embodiment 1 inorganic phosphobacteria strain YA-2

[0041] 1. The isolation and screening process of inorganic phosphate-solubilizing bacterial strain YA-2 is as follows:

[0042] (1) The soil was collected from the rhizosphere of weeds in the vegetation reclamation area of ​​Kunyang Phosphate Mine, Jinning County, Kunming City, Yunnan Province. The collected soil samples were put into a sealed bag by shaking the soil and immediately brought back to the laboratory for refrigeration for later use.

[0043] Inorganic phosphorus solid medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MnSO 4 .4H 2 O0.03g, FeSO 4 .7H 2 O 0.03g, MgSO 4 .7H 2 O 0.3g, Ca 3 (PO 4 ) 2 10g, 18g agar, 1000mL distilled water, pH7.0-7.5.

[0044] Inorganic phosphorus liquid medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MnSO 4 .4H 2 O0.03g, FeSO 4 .7H 2 O 0.03g, MgSO 4 .7H 2 O 0.3g, Ca 3 (PO 4 ) 2 10g, distilled water 1000mL...

Embodiment 2

[0053] The optimization of embodiment 2 culture medium

[0054] (5) Shake flask fermentation: Pick a ring of strains from the preservation medium, put them in a sterilized 150mL triangular flask containing 50mL seed medium, and place them at 28°C, 180r.min -1 Incubate overnight.

[0055] (6) Screening of the best carbon source and nitrogen source: on the basis of the original inorganic phosphorus liquid medium, the carbon source (glucose, sucrose, maltose, soluble starch, the addition amount is 10g / L) and the nitrogen source (ammonium sulfate 0.05g / L, potassium nitrate 0.0644g / L, ammonium nitrate 0.0303g / L, urea 0.0277g / L) for screening, 1*10 5 Sterilize under Pa condition for 30min, inoculate the seed liquid in the culture medium according to the inoculation amount of 1.5%, place the constant temperature shaker (180r.min -1 , 28°C) for 7 days on a shaking table, and the molybdenum blue colorimetric method was used to measure the available phosphorus content in the solution....

Embodiment 3

[0059] Example 3 bacterial strain acid phosphatase activity and organic acid total amount under different phosphorus sources

[0060] Determination of Phosphatase Activity of Bacteria under Different Phosphorus Sources

[0061]The making of standard curve: take 6 test tubes, absorb lg / L standard p-nitrophenol solution successively. 0, 1, 2, 3, 4, and 5mL were placed in test tubes respectively, and the volume was adjusted to 5mL with sterile water. Add 1 mL of 0.5 mol / L calcium chloride and 4 mL of sodium hydroxide to each test tube, shake and filter, and measure the absorbance of the filtrate at 420 nm. The number of micromoles that act on the substrate and release the product in IL fermentation broth (culture broth) per hour is called 1 enzyme activity unit, denoted as umolL -1 h -1 , abbreviated as Eu.

[0062] Take 1mL of fermentation broth, add 4mL of MUB with a pH value of 6.5, and add 0.025mol.L -1 Disodium p-nitrophenyl phosphate solution 1mL, shake for a few secon...

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Abstract

The invention discloses an Enterobacter strain with phosphorus-solubilizing ability and application thereof. The strain is classified as Enterobacter sp.YA-2 (Enterobacter sp.YA-2), and was deposited in the China Center for Type Culture Collection on March 7, 2016, with a preservation number of CCTCC NO: M 2016094. The bacterial strain provided by the invention is suitable for scrubbing tailings, and is also suitable for other insoluble phosphate and phosphorus-deficient soils. The strain can convert the insoluble phosphate in the scrubbing tailings into soluble phosphate, thereby improving the utilization efficiency of the scrubbing tailings. By studying its most suitable growth conditions and its salt and alkali resistance, temperature resistance, etc., and The bacterial strain is inoculated in the acidic red soil, the colonization ability of the bacterial strain is strong, and the dry weight and the fresh weight of the pakchoi are significantly improved, so it is suitable for increasing the output of crops.

Description

technical field [0001] The invention relates to the technical field of biodegradation of insoluble phosphate in phosphorus tailings, in particular to an enterobacter strain with phosphorus-dissolving ability and application thereof. Background technique [0002] Phosphorus is one of the essential nutrients for plants and an important component of compounds in plant cells. The total phosphorus content in plants accounts for 0.1-0.5% of the dry weight. There is no volatilization loss, and the leaching loss is also small. The excess phosphorus in the soil is basically fixed and becomes soil accumulation state P. Phosphate fertilizers are easily fixed in the soil and easily interact with Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, the interest rate of P fertilizer is not high in the current season, generally only 10-20% or lower, but its accumulation and utilization rate is relatively high. [0003] Since 2010, the problems faced by the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G22/15C12R1/01
CPCC12N1/205C12R2001/01
Inventor 达布希拉图曾婕李云驹张晖宗世荣张可欣李若兰冯晓军
Owner YUNNAN PHOSPHATE CHEM GROUP CORP
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