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Skin whitening and anti-skin aging butterfly orchid extractant

An anti-aging and skin whitening technology, applied in skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of limited efficacy, unevenness, and low proportion of active ingredients

Inactive Publication Date: 2017-01-11
上海奇易化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there have been creations of using Phalaenopsis extracts to make beauty products, the effectiveness of these extracts is still limited to a certain effect, that is, the proportion of active ingredients in the extracts extracted by currently known methods It may not be high or average, so there has not been an invention that contains extract ingredients that are both whitening and anti-aging, and have an excellent improvement effect

Method used

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  • Skin whitening and anti-skin aging butterfly orchid extractant
  • Skin whitening and anti-skin aging butterfly orchid extractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 Phalaenopsis extract

[0020] First, I purchased Phalaenopsis V3 from the market. Its scientific name is Phalaenopsis Sogo Yukidian ‘V3’ in English. The petals are picked, and the pedicel is cut to an appropriate length at the same time, washed several times with sterile water, and then placed in an oven set at 45-60° C. for drying before use.

[0021] In this example, multiple extraction methods will be carried out at the same time to compare and find out the extraction conditions with better effect extracts.

[0022] (1) Water extraction

[0023] Take the dried Phalaenopsis petals or pedicels as the extract, add pure water at a weight ratio of 1:10, stir and mix to form a mixture, and heat the mixture to 100°C for 90 minutes for extraction. Afterwards, the extracted mixture was filtered through filter paper with a pore size of 6 μm to obtain a water-soluble Phalaenopsis extract solution.

[0024] (2) Alcohol extraction after water ex...

Embodiment 2

[0031] Example 2 In vitro tyrosinase activity test

[0032] Add the aforementioned powdered Phalaenopsis extract to ddH 2 O, configured as a sample solution to be tested with a concentration of 10% (w / v) for standby use. Take 200 μl of the prepared sample solution to be tested and 800 μl of 0.1M phosphate buffer solution (pH 6.8), and inject them into the microtube. In the control group, the sample solution to be tested was replaced with an equal volume of 0.1M phosphate buffer solution. Afterwards, add 100 μl of 70 unit / ml mushroom tyrosinase to the microtubes of all the sample solutions to be tested and the control group, and finally add 900 μl of 25 mM L-DOPA, mix the solution well, and react at 37°C for 30 minutes . After the reaction was completed, the absorbance value at a wavelength of 405 nm was measured with a spectrophotometer, and the results are shown in Table 1.

[0033]If there is a component that inhibits tyrosinase in the sample to be tested, the original r...

Embodiment 3

[0041] Embodiment 3 antioxidant power test

[0042] In this example, the test on the antioxidant capacity was evaluated by the ability of Phalaenopsis extract to scavenge α,α-diphenyl–β–picrylhydrazyl (α,α-diphenyl–β–picrylhydrazyl, DPPH) free radicals Antioxidant capacity.

[0043] Take 0.2ml of the sample solution to be tested with different concentrations, add 1.8ml of freshly prepared 0.1mM DPPH methanol solution, shake and mix evenly, and react at room temperature for 30 minutes in a light-proof environment, and then use a spectrophotometer to detect the concentration at 517nm wavelength. absorbance value. In the control group, an equal volume of 0.1 mM DPPH ethanol solution was used instead of the sample solution to be tested, and then the same reaction test as above was carried out, and the results are shown in Table 2.

[0044] Since substances with antioxidant capacity will provide hydrogen to DPPH free radicals, the oxidation chain reaction will be inhibited. There...

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Abstract

The invention provides an skin whitening and anti-skin aging butterfly orchid extractant prepared by the following steps: a, providing butterfly orchid petal, pedicel or petal-pedicel mixed to-be-extracted material; b, mixing the to-be-extracted material with 95% (v / v) ethyl alcohol according to weight ratio of 1:20 to 1:5 so as to form a mixture, standing for 20-30 hours before extraction; c, filtering the mixture so as to obtain alcohol soluble butterfly orchid extractant solution. The butterfly orchid extractant can reduce tyrosinase and oxidation resisting activity, can whiten skin, and can prevent skin aging phenomenon.

Description

technical field [0001] The present invention relates to a Phalaenopsis extract used for skin whitening and anti-aging, in particular to a Phalaenopsis extract capable of inhibiting tyrosinase activity and having antioxidant activity. Background technique [0002] In today's society where the material life is becoming more and more affluent, modern people pay more and more attention to their personal appearance in addition to satisfying their physical needs. You can see this trend below. However, with regard to personal appearance, apart from the proportions or dimensions of the body, the amount or color of the hair, the health and color of the skin is the most widely used criterion for judging youth or proper maintenance. Whitening, clear, elastic and firm skin with a little luster has always been the beauty goal advertised by the medical aesthetics industry, and it is also the desire that consumers expect to achieve by themselves. [0003] The most important function of s...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61Q19/02A61Q19/08
CPCA61K8/97A61K2800/782A61Q19/02A61Q19/08
Inventor 谢铭峻
Owner 上海奇易化妆品有限公司
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