Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly evaluating lipid compositions in different milk fat globules

A milk fat globule and lipid technology, applied in the field of rapid evaluation of lipid composition in different milk fat globules, can solve the problems of inability to compare lipid composition of milk fat globules of different sizes, sample loss, etc., achieve short detection time and improve efficiency , cost reduction effect

Active Publication Date: 2017-01-04
JIANGNAN UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still impossible to compare the lipid composition of milk fat globules of different sizes, and it will cause some loss of samples
Routine detection methods also require specific chromatographic columns and separation conditions, and the detection conditions are strict

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly evaluating lipid compositions in different milk fat globules
  • Method for rapidly evaluating lipid compositions in different milk fat globules
  • Method for rapidly evaluating lipid compositions in different milk fat globules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Centrifuge 3000g of raw milk to be tested, pasteurized milk, pasteurized and homogenized milk, pasteurized and homogenized and spray-dried rehydrated milk at 2000rpm for 5min, and collect the top fat layer in the Disperse in the simulated milk ultrafiltrate to make its fat content 20%, then dilute ten times and place it on a concave glass slide, add 50 μL (w / v) of agarose with a low melting point mass concentration of 0.5% to fix the sample, Quickly and gently cover with a coverslip. Adjust the working parameters of the Raman spectroscopic analysis instrument: grating 600gr / mm, acquisition time: 10s, accumulation times: 1, use 50×LWD, lens acquisition wavelength range 400~3200cm -1 , each sample was scanned three times and the average value was taken as its Raman spectrum. For sample injection, the sample must be kept in a static state, and the lipid composition in different milk fat globules can be qualitatively analyzed through the characteristic Raman frequency. In...

Embodiment 2

[0029] Centrifuge 3000g of raw milk to be tested, pasteurized milk, pasteurized and homogenized milk, pasteurized, homogenized and spray-dried rehydrated milk at 2500rpm for 8min, and collect the top fat layer in the Disperse in the simulated milk ultrafiltrate to make its fat content 15%, then dilute ten times and place it on a concave glass slide, add 40 μL (w / v) of low melting point agarose to fix the sample, and cover it quickly and gently On a cover glass, the working parameters of the Raman spectroscopic analysis system are: grating 600gr / mm, acquisition time: 10s, accumulation times: 1, use 50×LWD, lens acquisition wavelength range 2600~3200cm -1 . Injection results see figure 2 , where the substances represented by different wavelengths are indicated. 2850: Cholesterol. Then by the ratio z=peak height I of the characteristic peak intensity of each component1 / peak height I 2 , to compare its content, the characteristic peak intensity is the average intensity of 3...

Embodiment 3

[0031] Centrifuge 3000g human colostrum, human mature milk, cow milk and goat milk fat globules respectively at 2500rpm for 10min, collect the top fat layer and disperse it in the simulated milk ultrafiltrate to make the fat content 20%, and then dilute ten times Add 45 μL (w / v) of low-melting point agarose to the concave slide to fix the sample, then quickly and gently cover it with a cover glass, and adjust the working parameters of the Raman spectroscopic analysis instrument: grating 600gr / mm, Acquisition time: 10s, accumulation times: 1, use 50×LWD, lens acquisition wavelength range 400~1800cm -1 . Injection results see image 3 , where the substances represented by different wavelengths are indicated. 860: phospholipids; 1010: fat-soluble carotenoids; 1160: fat-soluble carotenoids; 1270: C-H bonds in phospholipids; 1303: unsaturated bonds in phospholipids and cholesterol; 1443: saturated fatty acids; 1530: fat-soluble carotenoids 1658: unsaturated fatty acids; 1742: tr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a method for rapidly evaluating lipid compositions in different milk fat globules. The method includes the steps of collecting Raman spectrum charts of the milk fat globules of different sizes in a to-be-tested milk sample through a Raman spectrum analysis system after the to-be-tested milk sample is mixed with agarose, comparing characteristic peaks of the Raman spectrum charts, and judging the contents of lipid compositions through the comparison of strength of the characteristic peaks. By means of the Raman spectrum analysis system, compared with a traditional method, the method has the advantages that all complicated steps for extracting lipid in milk are omitted, spent detection time is short, cost is reduced, efficiency is improved, and the contents of lipid compositions in the milk fat globules of different sizes can be compared.

Description

technical field [0001] The invention belongs to the field of food analysis, in particular to a method for rapidly evaluating the lipid composition in different milk fat globules. Background technique [0002] Breast milk is the ideal food for newborn babies. However, the lack of breast milk is caused by some mothers' congenital defects or long-term work pressure. At this time, the milk is used to make breast milk substitutes. As an important nutrient in milk, fat can provide 40%-50% of energy and essential fatty acids for infants, and plays a vital role in the growth and development of infants. However, there are differences in lipid composition between cow's milk and breast milk. In addition, the lipid components in milk also have a great loss during processing. The fat in animal milk exists in the form of milk fat globules of different sizes. Among them, the triglyceride content accounts for more than 98%, which constitutes the center of the fat globule. The outside ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28G01N1/38
CPCG01N1/28G01N1/38G01N21/65
Inventor 王兴国姚云平金青哲孙聪闫媛媛邹孝强齐策韦伟黄健花刘睿杰常明王小三
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com