Quick test method

Abstract

The invention relates to a quick test method. Firstly, rice bran is partially degreased, lipoxidase in the rice bran which is partially degreased is extracted with a buffer solution, the lipoxidase extracting solution is added to a prepared phosphate-linoleic acid-polysorbate 20 substrate solution, changes of light-absorption photometric values are tested under 234 nm in 5 minutes, meanwhile, the protein content in the lipoxidase extracting solution is tested, and then activity of the lipoxidase in the rice bran can be obtained. According to the method, the activity of the lipoxidase in the rice bran is effectively activated through partial degreasing, the detection limit is raised, the buffer solution, the substrate solution and passivating crude enzyme serve as reference solutions, test errors of the crude enzyme are effectively eliminated, and complicated steps of passivating of the crude enzyme are eliminated. The method is easy and convenient to operate, precise in result, good in reproducibility, capable of being widely used for grain and oil quality test and control in grain processing enterprises, storage enterprises and grain and oil test organizations.

Description

technical field [0001] The invention relates to the field of intensive grain processing, in particular to a rapid detection method for the activity of lipoxygenase in rice bran and wheat germ, which are by-products of rice and wheat processing, and can be widely used in the quality inspection and control of grain processing enterprises, storage enterprises, and grain and oil testing institutions . Background technique [0002] At present, the lipoxygenase activity assay methods mainly include ultraviolet spectrophotometry, oxygen electrode method, etc., among which ultraviolet spectrophotometry is more commonly used. The spectrophotometric method measures the initial product of the enzyme-catalyzed reaction. The initial product generated by the reaction of the lipoxygenase-catalyzed substrate linoleic acid has the structure of a conjugated diene. The conjugated diene can be quantitatively generated by measuring the absorbance of the reaction system at 234nm. Diene content, ...

AI Technical Summary

Problems solved by technology

Existing studies have shown that various factors such as raw material type, reaction temperature, substrate concentration, Tween concentration, and reaction buffer system pH will affect the accuracy of lipoxygenase activity assay results.

[0004] So far, in domestic studies on rice bran lipoxygenase, the enzyme activity determination methods refer to the analysis methods of lipoxygenase in other plant materials, and there are no relevant research reports on specific analysis methods for lipoxygenase in rice bran