Kit for typing genes related to alcohol, folic acid and VD metabolic capability of human body

A technology of metabolism and genotyping, applied in the field of biotechnology related to invention, can solve problems such as amino acid sequence changes, affecting bone metabolism, etc., and achieve the effects of cost reduction, high sensitivity and accuracy, and low cost

Inactive Publication Date: 2017-01-04
HANGZHOU JILUO BIOLOGICAL PHARMA CO LTD
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AI Technical Summary

Problems solved by technology

The Fok I restriction site is located at the start of transcription, and its polymorphism can lead to changes in amino acid sequence, thereby affecting bone metabolism; Bsm I and Apa l restriction sites are located on the VIII intron, and their polymorphisms are not Affects the amino acid sequence of the VDR but affects intestinal uptake of calcium from food

Method used

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  • Kit for typing genes related to alcohol, folic acid and VD metabolic capability of human body
  • Kit for typing genes related to alcohol, folic acid and VD metabolic capability of human body
  • Kit for typing genes related to alcohol, folic acid and VD metabolic capability of human body

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Embodiment 1

[0032] 1. The composition of the kit.

[0033] A kit for genotyping related to human alcohol, folic acid and VD metabolic capacity in this embodiment, comprising: PCR amplification reaction solution, PCR product purification reagent, extension reaction reagent, extension product purification reagent, positive control solution and Table 3 shows the composition of the blank control solution and the test solution on the machine, a total of 7 tubes of reaction solutions.

[0034] The PCR reaction solution is contained in tube A, which is composed of 10×PCR buffer, dNTPs, HotStart Taq DNA polymerase and amplified primer mix I.

[0035] PCR product purification reagents are packed in tube B, which consists of 10× reaction buffer, shrimp alkaline phosphatase (SAP) and exonuclease (ExoI).

[0036] The extension reaction reagent is contained in tube C, which consists of 10×SNaPshot Multiplex mixture and extension primer mixture II.

[0037] The extension product purification reagent ...

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Abstract

The invention belongs to the field of biotechnology and relates to a kit for typing genes related to alcohol, folic acid and VD metabolic capability of a human body based on a SNaPshot technique. The kit can be used for once quickly detecting the gene types of ADH1B gene G1434A locus, ALDH2 gene G1510A locus, MTHFR gene A1298C locus and C677T locus, MTRR gene A66G locus and VDR gene Bsm I, Apa I and Fok I locus. The kit comprises a PCR amplification reagent, a PCR product purifying reagent, an extending reaction reagent, an extension reaction purifying reagent and an electrophoresis reagent, wherein the PCR amplification reagent comprises an amplimer mixed solution I of 8 locus of 5 related genes and the extension reaction purifying reagent comprises an amplimer mixed solution II of 8 locus of 5 related genes. The kit provided by the invention can once quickly, accurately, sensitively and economically detect the related gene type of human metabolic capability including alcohol metabolic capability, folic acid utilizing capability and vitamin D utilizing capability and can supply scientific and reliable basis to human health management.

Description

technical field [0001] The invention relates to the field of gene polymorphism detection in the field of biotechnology, in particular to a type detection of the ADH1B gene G143A site and the ALDH2 gene G1510A site related to the alcohol metabolism of the Chinese population, and the MTHFR related to the metabolism and absorption of folic acid in the Chinese population Gene C677T site and A1298C site polymorphism and MTRR gene A66G site typing detection and VDR gene Bsm I, Apa I, Fok I site typing detection related to VD (vitamin D) metabolism ability of Chinese population Reagent test kit. Background technique [0002] China is a big consumer of wine, wine is a mixture of various chemical components, ethanol is its main component. After drinking, ethanol quickly enters the blood through the capillaries of the stomach and small intestine, and then is transported to the liver for digestion through the blood vessels. Alcoholism is caused by ethanol entering the human body and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156C12Q2600/16
Inventor 马卧龙范嘉庚李鹏飞李洲
Owner HANGZHOU JILUO BIOLOGICAL PHARMA CO LTD
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