Application of cyclic dinucleotide cgamp in preparation of anti-senile dementia medicine

A technology for senile dementia and drugs, applied in the field of biomedicine, can solve problems such as unclear mechanisms for identifying and clearing numbers

Active Publication Date: 2020-02-07
HANGZHOU XINGAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cell-free DNA in the cytoplasm has been regarded as a potential danger signal by the host's natural immune system, but the mechanism by which the immune system recognizes and clears these danger signals is still unclear

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1: Preparation of cGAMP

[0008] cGAMP (cyclized-GMP-AMP) was synthesized by cyclized cGMP-AMP dinucleotide synthetase (cGAS) under activation conditions after binding to dsDNA according to literature method. The purity is above 98%. (PingweiLi, et al., Immunity, 2013, 39(6), 1019-1031.)

Embodiment 2

[0009] Example 2: Acute Toxicity Study of cGAMP

[0010] Experimental Materials

[0011] 20 ICR mice (purchased from Shanghai Slack Experimental Animal Co., Ltd. [Experimental animal quality certificate number: SCXK (Shanghai) 2007-0005]), half male and half male, weighing 18-22 g, the animals were fed with pellet feed, free Eat and drink. cGAMP was prepared from Example 1, and was prepared into a solution with a concentration of 200 mg / mL with physiological saline.

[0012] experimental method

[0013] ICR mice were injected with 5 mg / kg of cGAMP into the tail vein according to body weight, and the toxic reaction and death of the mice were observed within 14 days after administration. It was found that after a single tail vein injection in mice, the mice moved normally. Within 14 days after intravenous injection of cGAMP, the mice did not die. On the 15th day, all the mice were sacrificed, dissected, and all organs were checked, and no obvious lesions were found.

[0014...

Embodiment 3

[0016] Example 3: cGAMP has the effect of immune prevention of tumor

[0017] Experimental Materials

[0018] cGAMP was prepared from Example 1, and was prepared into a solution with a concentration of 200 mg / mL with physiological saline. 20 ICR mice (purchased from Shanghai Slack Experimental Animal Co., Ltd. [Experimental Animal Quality Certificate No.: SCXK (Shanghai) 2007-0005]), male, weighing 18-22 g, were fed with pellet feed, free to eat and drink. drinking water. Tumor cell lines: human gastric cancer cell line MNK-45, human lung adenocarcinoma cell line A549, human colon cancer cell line Lovo, human liver cancer cell line SMMC-7721, human prostate cancer cell line PC-3, human pancreatic cancer cell line SW1990, All were purchased from the Cell Bank of the Chinese Academy of Sciences.

[0019] experimental method

[0020] Negative control, physiological saline solution; positive control, 5-fluorouracil (5-FU) anticancer reagent (dose 5 mg / kg). cGAMP was injected ...

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PUM

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Abstract

The invention belongs to the technical field of medicine, and specifically relates to applications of cyclic dinucleotide cGAMP in preparation of health products. According to the present invention, the research results show that the cyclic dinucleotide cGAMP as the human body natural immune signal pathway activator and the natural immune system enhancer can achieve the important disease preventing and body building immune defense effects of multiple virus infection prevention, tumor occurrence and development inhibition, Alzheimer disease prevention and the like by enhancing the immunity of the innate immune system; the acute animal toxicity test results show that the cGAMP does not present the significant acute toxicity, such that the cGAMP used as the endogenous second messenger capable of regulating the natural immune reaction has potential applications of virus infection prevention, virus infection resistance and tumor resistance; and the cGAMP can be used for preparing the additive of health products or special dietary.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of a cyclic dinucleotide (cGAMP) in the preparation of anti-senile dementia medicine. Background technique [0002] The human immune system can recognize and eliminate "non-self" antigens and exert immune protection, including anti-infection immunity and tumor immunity. The human body's immune system can prevent and eliminate pathogen invasion, neutralize toxic metabolites or other foreign bodies. When the immune surveillance ability is insufficient, it can lead to the occurrence of tumors and persistent infection of viruses. [0003] Cell-free DNA in the cytoplasm has been regarded as a potential danger signal by the host's natural immune system, but the mechanism by which the immune system recognizes and clears these danger signals is still unclear. In recent years, studies have found that DNA sensors (DNAsensors) are the bridge between host DN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7084A61P37/04A61P25/28A61P35/00A23L33/13
Inventor 张跃茹向道凤
Owner HANGZHOU XINGAO BIOTECH CO LTD
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