Method for treating and alleviating cadmium adverse environmental stress damage to corn seedlings by aid of exogenous brassinolide

Abstract

The invention discloses a method for treating and alleviating cadmium adverse environmental stress damage to corn seedlings by the aid of exogenous brassinolide. The method mainly includes treating seeds and then planting the seeds; selecting 120 corn seedlings with excellent and consistent growth vigor and carrying out cadmium stress experiments on the corn seedlings; setting serial Cd<2+> stress concentration gradient, treating the corn seedlings by the aid of Hoagland nutrient solution, cultivating the corn seedlings for 10 d and then screening the optimal Cd<2+> stress concentration according to relative growth rates of the seedlings; setting 6 types of treatment solution by the aid of the brassinolide for Cd<2+> toxicity alleviating effect experiments and irrigating the corresponding 6 types of treatment solution to the planted corn seedlings; measuring various indexes of the corn seedlings after the corn seedlings are treated for 7 d; analyzing results and screening the most appropriate EBR (brassinolide) concentration. The method has the advantages that growth of the corn seedlings under stress of heavy metal cadmium can be effectively promoted by the exogenous brassinolide, accumulation of biomass of the corn seedlings can be increased, the activity of antioxidant enzymes in corn leaves under the stress of the heavy metal cadmium can be improved, the content of in-vivo active oxygen can be increased, electrolyte leakage under the stress can be reduced, and accordingly damage to plants under the stress of the heavy metal cadmium can be relieved.

Description

technical field [0001] The invention belongs to the technical field of agricultural application, and in particular relates to a method for alleviating the growth of corn seedlings under heavy metal cadmium adversity stress by exogenous 24-epibrassinolide treatment. Background technique [0002] Cadmium (Cd) is a non-essential element for plants. It has high mobility and toxicity. It is potentially harmful to various biological organisms and can cause many diseases in humans. It is regarded as one of the most threatening heavy metals to humans. With the development of mining, smelting and cadmium processing industries and the extensive use of heavy metal-containing organic fertilizers and chemical fertilizers in agricultural production, soil cadmium pollution has become increasingly serious. Studies have shown that the accumulation of cadmium in the soil is very harmful to plants. When cadmium enters the plant and accumulates to a certain level, it will destroy the structure ...

AI Technical Summary

Problems solved by technology

[0004] Corn is one of the most widely planted food crops on heavy metal-polluted soils. There are existing reports on some hormone mitigation methods for heavy metal toxicity in corn, such as salicylic acid and nitric oxide, and brassinosteroids to alleviate heavy metal adversity stress The method is rarely reported, and it is rarely used in actual agricultural production

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