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Rhodobacter sphaeroides strain and preparation method and application thereof

A technology of Rhodobacter sphaeroides and strains, which is applied in the field of production of Rhodobacter sphaeroides coenzyme Q10, can solve the problem of low yield of products with genetic characteristics of strains, and achieve the effects of increasing production and reducing costs

Active Publication Date: 2016-11-23
FUJIAN FUKANG PHARMA
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  • Abstract
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Problems solved by technology

However, the main factors restricting the production of coenzyme Q10 by microbial fermentation are the low yield of products caused by the genetic characteristics of strains and complex fermentation regulation.
At present, there is no report on enhancing the production of coenzyme Q10 in Rhodobacter sphaeroides by modifying the chorismate pathway, that is, the quinone ring synthesis process

Method used

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  • Rhodobacter sphaeroides strain and preparation method and application thereof
  • Rhodobacter sphaeroides strain and preparation method and application thereof
  • Rhodobacter sphaeroides strain and preparation method and application thereof

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Embodiment

[0043] 1. Strains and experimental materials

[0044] All the strains and materials in this example were purchased and sold in the market. For example, Rhodobacter sphaeroides 2.4.1 was purchased from ATCC (strain number ATCC-17023), and Acinetobacter calcoaceticus was purchased from ATCC (strain number ATCC-31926). Plasmid and genome extraction kit were purchased from TIANGEN company. The PCR product recovery kit was purchased from Omega, USA. T 4 Ligase was purchased from NEB, USA. The chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd., all of which were of analytical grade.

[0045] 2. Construction of pBBR1MCS-2-pcaK vector:

[0046] The p-hydroxybenzoic acid transporter (pcaK) gene was cloned from the genome of Acinetobacter calcium acetate:

[0047] Acinetobacter calcium acetate was inoculated into a 50mL Erlenmeyer flask containing LB medium and cultured overnight for 8 hours. The bacteria were collected by centrifugation. The genome of Acinetobacter...

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Abstract

The invention provides a rhodobacter sphaeroides strain and a preparation method and application thereof. P-hydroxybenzoic acid transportprotein pcaK gene from acinetobacter calcoaceticus is introduced into the rhodobacter sphaeroides strain, the ability of taking p-hydroxybenzoic acid from an extracellular medium of the rhodobacter sphaeroides strain is enhanced, and therefore, the yield of synthesizing coenzyme Q10 in the rhodobacter sphaeroides strain is improved. By the rhodobacter sphaeroides strain, the shortcomings that in production of coenzyme Q10 by microbiological fermentation, the yield is low, the production cost is high and the like are overcome, the yield of the intracellular coenzyme Q10 of rhodobacter sphaeroides can be improved remarkably, and the cost of industrialized production of the coenzyme Q10 is reduced.

Description

Technical field [0001] The invention relates to the production field of Rhodobacter sphaeroides coenzyme Q10, in particular to a Rhodobacter sphaeroides coenzyme Q10 strain, a preparation method thereof and a method for producing Rhodobacter sphaeroides coenzyme Q10. Background technique [0002] Coenzyme Q10 (Coenzyme Q10) is a kind of fat-soluble quinone compound that exists in nature. Its structure is synthesized from quinone derivatives and multiple isoprene monomers, but coenzyme Q10 from different sources has side chain isoprene units. The number is different, humans and mammals are 10 isoamylene units. As an important hydrogen transmitter in the respiratory chain of biological cells, Coenzyme Q10 participates in cell energy metabolism. Participating in energy production and activation in human body cells is the most effective antioxidant ingredient to prevent arteriosclerosis. In addition, Coenzyme Q10 plays an important role in anti-tumor, anti-aging effects, treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/70C12N15/74C12P7/66C12R1/01
CPCC07K14/212C12P7/66
Inventor 祁峰张媛黄建忠江贤章张明亮邹丽梅
Owner FUJIAN FUKANG PHARMA
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